Polysulfides (H2Sn) produced from the interaction of hydrogen sulfide (H2S) and nitric oxide (NO) activate TRPA1 channels

Ryo Miyamoto, Shin Koike, Yoko Takano, Norihiro Shibuya, Yuka Kimura, Kenjiro Hanaoka, Yasuteru Urano, Yuki Ogasawara, Hideo Kimura

Research output: Contribution to journalArticlepeer-review

72 Citations (Scopus)

Abstract

Hydrogen sulfide (H2S) exerts synergistic effects with another gaseous signaling molecule nitric oxide (NO) on ion channels and vasculature. However, the mechanism of the synergy is not well understood. Here, we show that the interaction between H2S and NO generates polysulfides (H2Sn), which activate transient receptor potential ankyrin 1 (TRPA1) channels. High performance liquid chromatography with tandem mass spectrometry analysis, along with the imaging of intracellular Ca2+ and H2Sn, showed that H2Sn and their effects were abolished by cyanolysis and by reducing substances such as dithiothreitol (DTT), cysteine, and glutathione (GSH). However, the effects of nitroxyl or nitrosopersulfide, other potential products of H2S and NO interaction, are not affected by cyanolysis or reducing substances. This study demonstrates that H2Sn are products of synergy between H2S and NO and provides a new insight into the signaling mechanisms.

Original languageEnglish
Article number45995
JournalScientific reports
Volume7
DOIs
Publication statusPublished - 2017 Apr 5
Externally publishedYes

ASJC Scopus subject areas

  • General

Fingerprint

Dive into the research topics of 'Polysulfides (H<sub>2</sub>S<sub>n</sub>) produced from the interaction of hydrogen sulfide (H<sub>2</sub>S) and nitric oxide (NO) activate TRPA1 channels'. Together they form a unique fingerprint.

Cite this