Polysulfides (H2Sn) produced from the interaction of hydrogen sulfide (H2S) and nitric oxide (NO) activate TRPA1 channels

Ryo Miyamoto; Shin Koike; Yoko Takano; Norihiro Shibuya; Yuka Kimura; Kenjiro Hanaoka; Yasuteru Urano; Yuki Ogasawara; Hideo Kimura

doi:10.1038/srep45995

Polysulfides (H₂S_n) produced from the interaction of hydrogen sulfide (H₂S) and nitric oxide (NO) activate TRPA1 channels

Ryo Miyamoto, Shin Koike, Yoko Takano, Norihiro Shibuya, Yuka Kimura, Kenjiro Hanaoka, Yasuteru Urano, Yuki Ogasawara, Hideo Kimura

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93 Citations (Scopus)

Abstract

Hydrogen sulfide (H₂S) exerts synergistic effects with another gaseous signaling molecule nitric oxide (NO) on ion channels and vasculature. However, the mechanism of the synergy is not well understood. Here, we show that the interaction between H₂S and NO generates polysulfides (H₂S_n), which activate transient receptor potential ankyrin 1 (TRPA1) channels. High performance liquid chromatography with tandem mass spectrometry analysis, along with the imaging of intracellular Ca²⁺ and H₂S_n, showed that H₂S_n and their effects were abolished by cyanolysis and by reducing substances such as dithiothreitol (DTT), cysteine, and glutathione (GSH). However, the effects of nitroxyl or nitrosopersulfide, other potential products of H₂S and NO interaction, are not affected by cyanolysis or reducing substances. This study demonstrates that H₂S_n are products of synergy between H₂S and NO and provides a new insight into the signaling mechanisms.

Original language	English
Article number	45995
Journal	Scientific reports
Volume	7
DOIs	https://doi.org/10.1038/srep45995
Publication status	Published - 2017 Apr 5
Externally published	Yes

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title = "Polysulfides (H2Sn) produced from the interaction of hydrogen sulfide (H2S) and nitric oxide (NO) activate TRPA1 channels",

abstract = "Hydrogen sulfide (H2S) exerts synergistic effects with another gaseous signaling molecule nitric oxide (NO) on ion channels and vasculature. However, the mechanism of the synergy is not well understood. Here, we show that the interaction between H2S and NO generates polysulfides (H2Sn), which activate transient receptor potential ankyrin 1 (TRPA1) channels. High performance liquid chromatography with tandem mass spectrometry analysis, along with the imaging of intracellular Ca2+ and H2Sn, showed that H2Sn and their effects were abolished by cyanolysis and by reducing substances such as dithiothreitol (DTT), cysteine, and glutathione (GSH). However, the effects of nitroxyl or nitrosopersulfide, other potential products of H2S and NO interaction, are not affected by cyanolysis or reducing substances. This study demonstrates that H2Sn are products of synergy between H2S and NO and provides a new insight into the signaling mechanisms.",

author = "Ryo Miyamoto and Shin Koike and Yoko Takano and Norihiro Shibuya and Yuka Kimura and Kenjiro Hanaoka and Yasuteru Urano and Yuki Ogasawara and Hideo Kimura",

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doi = "10.1038/srep45995",

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AU - Miyamoto, Ryo

AU - Koike, Shin

AU - Takano, Yoko

AU - Shibuya, Norihiro

AU - Kimura, Yuka

AU - Hanaoka, Kenjiro

AU - Urano, Yasuteru

AU - Ogasawara, Yuki

AU - Kimura, Hideo

PY - 2017/4/5

Y1 - 2017/4/5

N2 - Hydrogen sulfide (H2S) exerts synergistic effects with another gaseous signaling molecule nitric oxide (NO) on ion channels and vasculature. However, the mechanism of the synergy is not well understood. Here, we show that the interaction between H2S and NO generates polysulfides (H2Sn), which activate transient receptor potential ankyrin 1 (TRPA1) channels. High performance liquid chromatography with tandem mass spectrometry analysis, along with the imaging of intracellular Ca2+ and H2Sn, showed that H2Sn and their effects were abolished by cyanolysis and by reducing substances such as dithiothreitol (DTT), cysteine, and glutathione (GSH). However, the effects of nitroxyl or nitrosopersulfide, other potential products of H2S and NO interaction, are not affected by cyanolysis or reducing substances. This study demonstrates that H2Sn are products of synergy between H2S and NO and provides a new insight into the signaling mechanisms.

AB - Hydrogen sulfide (H2S) exerts synergistic effects with another gaseous signaling molecule nitric oxide (NO) on ion channels and vasculature. However, the mechanism of the synergy is not well understood. Here, we show that the interaction between H2S and NO generates polysulfides (H2Sn), which activate transient receptor potential ankyrin 1 (TRPA1) channels. High performance liquid chromatography with tandem mass spectrometry analysis, along with the imaging of intracellular Ca2+ and H2Sn, showed that H2Sn and their effects were abolished by cyanolysis and by reducing substances such as dithiothreitol (DTT), cysteine, and glutathione (GSH). However, the effects of nitroxyl or nitrosopersulfide, other potential products of H2S and NO interaction, are not affected by cyanolysis or reducing substances. This study demonstrates that H2Sn are products of synergy between H2S and NO and provides a new insight into the signaling mechanisms.

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Polysulfides (H₂S_n) produced from the interaction of hydrogen sulfide (H₂S) and nitric oxide (NO) activate TRPA1 channels

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