Polyunsaturated fatty acid saturation by gut lactic acid bacteria affecting host lipid composition

Shigenobu Kishino, Michiki Takeuchi, Si Bum Park, Akiko Hirata, Nahoko Kitamura, Jun Kunisawa, Hiroshi Kiyono, Ryo Iwamoto, Yosuke Isobe, Makoto Arita, Hiroyuki Arai, Kazumitsu Ueda, Jun Shima, Satomi Takahashi, Kenzo Yokozeki, Sakayu Shimizu, Jun Ogawa

Research output: Contribution to journalArticle

109 Citations (Scopus)

Abstract

In the representative gut bacterium Lactobacillus plantarum, we identified genes encoding the enzymes involved in a saturation metabolism of polyunsaturated fatty acids and revealed in detail the metabolic pathway that generates hydroxy fatty acids, oxo fatty acids, conjugated fatty acids, and partially saturated transfatty acids as intermediates. Furthermore, we observed these intermediates, especially hydroxy fatty acids, in host organs. Levels of hydroxy fatty acids were much higher in specific pathogen-free mice than in germ-free mice, indicating that these fatty acids are generated through polyunsaturated fatty acids metabolism of gastrointestinal microorganisms. These findings suggested that lipid metabolism by gastrointestinal microbes affects the health of the host by modifying fatty acid composition.

Original languageEnglish
Pages (from-to)17808-17813
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume110
Issue number44
DOIs
Publication statusPublished - 2013 Oct 29
Externally publishedYes

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Unsaturated Fatty Acids
Lactic Acid
Fatty Acids
Bacteria
Lipids
Hydroxy Acids
Keto Acids
Specific Pathogen-Free Organisms
Lactobacillus plantarum
Metabolic Networks and Pathways
Lipid Metabolism
Acids
Health
Enzymes
Genes

ASJC Scopus subject areas

  • General

Cite this

Polyunsaturated fatty acid saturation by gut lactic acid bacteria affecting host lipid composition. / Kishino, Shigenobu; Takeuchi, Michiki; Park, Si Bum; Hirata, Akiko; Kitamura, Nahoko; Kunisawa, Jun; Kiyono, Hiroshi; Iwamoto, Ryo; Isobe, Yosuke; Arita, Makoto; Arai, Hiroyuki; Ueda, Kazumitsu; Shima, Jun; Takahashi, Satomi; Yokozeki, Kenzo; Shimizu, Sakayu; Ogawa, Jun.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, No. 44, 29.10.2013, p. 17808-17813.

Research output: Contribution to journalArticle

Kishino, S, Takeuchi, M, Park, SB, Hirata, A, Kitamura, N, Kunisawa, J, Kiyono, H, Iwamoto, R, Isobe, Y, Arita, M, Arai, H, Ueda, K, Shima, J, Takahashi, S, Yokozeki, K, Shimizu, S & Ogawa, J 2013, 'Polyunsaturated fatty acid saturation by gut lactic acid bacteria affecting host lipid composition', Proceedings of the National Academy of Sciences of the United States of America, vol. 110, no. 44, pp. 17808-17813. https://doi.org/10.1073/pnas.1312937110
Kishino, Shigenobu ; Takeuchi, Michiki ; Park, Si Bum ; Hirata, Akiko ; Kitamura, Nahoko ; Kunisawa, Jun ; Kiyono, Hiroshi ; Iwamoto, Ryo ; Isobe, Yosuke ; Arita, Makoto ; Arai, Hiroyuki ; Ueda, Kazumitsu ; Shima, Jun ; Takahashi, Satomi ; Yokozeki, Kenzo ; Shimizu, Sakayu ; Ogawa, Jun. / Polyunsaturated fatty acid saturation by gut lactic acid bacteria affecting host lipid composition. In: Proceedings of the National Academy of Sciences of the United States of America. 2013 ; Vol. 110, No. 44. pp. 17808-17813.
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