Porcupine Controls Hippocampal AMPAR Levels, Composition, and Synaptic Transmission

Nadine Erlenhardt, Hong Yu, Kavitha Abiraman, Tokiwa Yamasaki, Jacques I. Wadiche, Susumu Tomita, David S. Bredt

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

AMPA receptor (AMPAR) complexes contain auxiliary subunits that modulate receptor trafficking and gating. In addition to the transmembrane AMPAR regulatory proteins (TARPs) and cornichons (CNIH-2/3), recent proteomic studies identified a diverse array of additional AMPAR-associated transmembrane and secreted partners. We systematically surveyed these and found that PORCN and ABHD6 increase GluA1 levels in transfected cells. Knockdown of PORCN in rat hippocampal neurons, which express it in high amounts, selectively reduces levels of all tested AMPAR complex components. Regulation of AMPARs is independent of PORCN's membrane-associated O-acyl transferase activity. PORCN knockdown in hippocampal neurons decreases AMPAR currents and accelerates desensitization and leads to depletion of TARP γ-from AMPAR complexes.

Original languageEnglish
Pages (from-to)782-794
Number of pages13
JournalCell Reports
Volume14
Issue number4
DOIs
Publication statusPublished - 2016 Feb 2
Externally publishedYes

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Porcupines
AMPA Receptors
Synaptic Transmission
Chemical analysis
Neurons
Transferases
Proteomics
Rats
Proteins
Membranes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Erlenhardt, N., Yu, H., Abiraman, K., Yamasaki, T., Wadiche, J. I., Tomita, S., & Bredt, D. S. (2016). Porcupine Controls Hippocampal AMPAR Levels, Composition, and Synaptic Transmission. Cell Reports, 14(4), 782-794. https://doi.org/10.1016/j.celrep.2015.12.078

Porcupine Controls Hippocampal AMPAR Levels, Composition, and Synaptic Transmission. / Erlenhardt, Nadine; Yu, Hong; Abiraman, Kavitha; Yamasaki, Tokiwa; Wadiche, Jacques I.; Tomita, Susumu; Bredt, David S.

In: Cell Reports, Vol. 14, No. 4, 02.02.2016, p. 782-794.

Research output: Contribution to journalArticle

Erlenhardt, N, Yu, H, Abiraman, K, Yamasaki, T, Wadiche, JI, Tomita, S & Bredt, DS 2016, 'Porcupine Controls Hippocampal AMPAR Levels, Composition, and Synaptic Transmission', Cell Reports, vol. 14, no. 4, pp. 782-794. https://doi.org/10.1016/j.celrep.2015.12.078
Erlenhardt, Nadine ; Yu, Hong ; Abiraman, Kavitha ; Yamasaki, Tokiwa ; Wadiche, Jacques I. ; Tomita, Susumu ; Bredt, David S. / Porcupine Controls Hippocampal AMPAR Levels, Composition, and Synaptic Transmission. In: Cell Reports. 2016 ; Vol. 14, No. 4. pp. 782-794.
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