Possibility for neurogenesis in substantia nigra of parkinsonian brain

Kenji Yoshimi, Yong Ri Ren, Tatsunori Seki, Masanori Yamada, Hideki Ooizumi, Masafumi Onodera, Yuko Saito, Shigeo Murayama, Hideyuki Okano, Yoshikuni Mizuno, Hideki Mochizuki

Research output: Contribution to journalArticle

105 Citations (Scopus)

Abstract

Recent studies of enhanced hippocampal neurogenesis by antidepressants suggest enhancement of neurogenesis is a potentially effective therapy in neurodegenerative diseases. In this study, we evaluated nigral neurogenesis in animals and autopsy brains including patients with Parkinson's disease (PD). First, proliferating cells in substantia nigra were labeled with retroviral transduction of green fluorescent protein, which is an efficient method to label neuronal stem cells. Subsequent differentiation of labeled cells was followed; many transduced cells became microglia, but no differentiation into tyrosine hydroxylase-positive neurons was detected at 4 weeks after injection, in both intact rodents and those treated with 1-methyl-4-phenyl-1,2,3,6- tetrahydropyridine. Second, polysialic acid (PSA)-like immunoreactivity, indicative of newly differentiated neurons, was detected in the substantia nigra of rodent, primate, and human midbrains. A large number of PSA-positive cells were detected in the substantia nigra pars reticulata of some patients with PD. In rats and a macaque monkey, the dopamine-depleted hemispheres showed more PSA staining than the intact side. A small number of tyrosine hydroxylase-positive cells were PSA-positive. Our results suggest enhanced neural reconstruction in PD, which may be important in the design of new therapies against the progression of PD.

Original languageEnglish
Pages (from-to)31-40
Number of pages10
JournalAnnals of Neurology
Volume58
Issue number1
DOIs
Publication statusPublished - 2005 Jul

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Neurogenesis
Substantia Nigra
Parkinson Disease
Brain
Tyrosine 3-Monooxygenase
Rodentia
Neurons
1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine
Microglia
Macaca
Mesencephalon
Green Fluorescent Proteins
Neurodegenerative Diseases
Primates
Antidepressive Agents
Haplorhini
Cell Differentiation
Autopsy
Dopamine
Stem Cells

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Yoshimi, K., Ren, Y. R., Seki, T., Yamada, M., Ooizumi, H., Onodera, M., ... Mochizuki, H. (2005). Possibility for neurogenesis in substantia nigra of parkinsonian brain. Annals of Neurology, 58(1), 31-40. https://doi.org/10.1002/ana.20506

Possibility for neurogenesis in substantia nigra of parkinsonian brain. / Yoshimi, Kenji; Ren, Yong Ri; Seki, Tatsunori; Yamada, Masanori; Ooizumi, Hideki; Onodera, Masafumi; Saito, Yuko; Murayama, Shigeo; Okano, Hideyuki; Mizuno, Yoshikuni; Mochizuki, Hideki.

In: Annals of Neurology, Vol. 58, No. 1, 07.2005, p. 31-40.

Research output: Contribution to journalArticle

Yoshimi, K, Ren, YR, Seki, T, Yamada, M, Ooizumi, H, Onodera, M, Saito, Y, Murayama, S, Okano, H, Mizuno, Y & Mochizuki, H 2005, 'Possibility for neurogenesis in substantia nigra of parkinsonian brain', Annals of Neurology, vol. 58, no. 1, pp. 31-40. https://doi.org/10.1002/ana.20506
Yoshimi K, Ren YR, Seki T, Yamada M, Ooizumi H, Onodera M et al. Possibility for neurogenesis in substantia nigra of parkinsonian brain. Annals of Neurology. 2005 Jul;58(1):31-40. https://doi.org/10.1002/ana.20506
Yoshimi, Kenji ; Ren, Yong Ri ; Seki, Tatsunori ; Yamada, Masanori ; Ooizumi, Hideki ; Onodera, Masafumi ; Saito, Yuko ; Murayama, Shigeo ; Okano, Hideyuki ; Mizuno, Yoshikuni ; Mochizuki, Hideki. / Possibility for neurogenesis in substantia nigra of parkinsonian brain. In: Annals of Neurology. 2005 ; Vol. 58, No. 1. pp. 31-40.
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