Abstract
A cleavage product of α-fodrin may be an important organ-specific autoantigen in the pathogenesis of Sjogren's syndrome (SS), but the mechanisms of α-fodrin cleavage remain unclear. Since EBV has been implicated in the pathogenesis of SS, we determined whether EBV activation could induce the SS-specific 120-kDa autoantigen α-fodrin. ZEBRA mRNA expression, a marker for activation of the lytic cycle of EBV, was found in the salivary gland tissues from SS patients, but not in those from control individuals. ZEBRA-expressing lymphoid cells were also found in the SS glands in double-stained immunohistochemistry. Furthermore, a significant link between production of Abs against 120-kDa α-fodrin and reactivated EBV Ag was found in sera from patients with SS, but not in those from control individuals. EBV-activated lymphoid cells showed specific α-fodrin cleavage to the expected 120-kDa fragments in vitro. Pretreatment with caspase inhibitors inhibited cleavage of α-fodrin. Thus, an increase in apoptotic protease activities induced by EBV reactivation may be involved in the progression of α-fodrin proteolysis in the development of SS.
| Original language | English |
|---|---|
| Pages (from-to) | 5801-5809 |
| Number of pages | 9 |
| Journal | Journal of Immunology |
| Volume | 166 |
| Issue number | 9 |
| Publication status | Published - 2001 May 1 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Immunology
Cite this
Possible involvement of EBV-mediated α-fodrin cleavage for organ-specific autoantigen in Sjogren's syndrome. / Inoue, H.; Tsubota, Kazuo; Ono, M.; Kizu, Y.; Mizuno, F.; Takada, K.; Yamada, K.; Yanagi, K.; Hayashi, Y.; Saito, I.
In: Journal of Immunology, Vol. 166, No. 9, 01.05.2001, p. 5801-5809.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Possible involvement of EBV-mediated α-fodrin cleavage for organ-specific autoantigen in Sjogren's syndrome
AU - Inoue, H.
AU - Tsubota, Kazuo
AU - Ono, M.
AU - Kizu, Y.
AU - Mizuno, F.
AU - Takada, K.
AU - Yamada, K.
AU - Yanagi, K.
AU - Hayashi, Y.
AU - Saito, I.
PY - 2001/5/1
Y1 - 2001/5/1
N2 - A cleavage product of α-fodrin may be an important organ-specific autoantigen in the pathogenesis of Sjogren's syndrome (SS), but the mechanisms of α-fodrin cleavage remain unclear. Since EBV has been implicated in the pathogenesis of SS, we determined whether EBV activation could induce the SS-specific 120-kDa autoantigen α-fodrin. ZEBRA mRNA expression, a marker for activation of the lytic cycle of EBV, was found in the salivary gland tissues from SS patients, but not in those from control individuals. ZEBRA-expressing lymphoid cells were also found in the SS glands in double-stained immunohistochemistry. Furthermore, a significant link between production of Abs against 120-kDa α-fodrin and reactivated EBV Ag was found in sera from patients with SS, but not in those from control individuals. EBV-activated lymphoid cells showed specific α-fodrin cleavage to the expected 120-kDa fragments in vitro. Pretreatment with caspase inhibitors inhibited cleavage of α-fodrin. Thus, an increase in apoptotic protease activities induced by EBV reactivation may be involved in the progression of α-fodrin proteolysis in the development of SS.
AB - A cleavage product of α-fodrin may be an important organ-specific autoantigen in the pathogenesis of Sjogren's syndrome (SS), but the mechanisms of α-fodrin cleavage remain unclear. Since EBV has been implicated in the pathogenesis of SS, we determined whether EBV activation could induce the SS-specific 120-kDa autoantigen α-fodrin. ZEBRA mRNA expression, a marker for activation of the lytic cycle of EBV, was found in the salivary gland tissues from SS patients, but not in those from control individuals. ZEBRA-expressing lymphoid cells were also found in the SS glands in double-stained immunohistochemistry. Furthermore, a significant link between production of Abs against 120-kDa α-fodrin and reactivated EBV Ag was found in sera from patients with SS, but not in those from control individuals. EBV-activated lymphoid cells showed specific α-fodrin cleavage to the expected 120-kDa fragments in vitro. Pretreatment with caspase inhibitors inhibited cleavage of α-fodrin. Thus, an increase in apoptotic protease activities induced by EBV reactivation may be involved in the progression of α-fodrin proteolysis in the development of SS.
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UR - http://www.scopus.com/inward/citedby.url?scp=0035340982&partnerID=8YFLogxK
M3 - Article
C2 - 11313424
AN - SCOPUS:0035340982
VL - 166
SP - 5801
EP - 5809
JO - Journal of Immunology
JF - Journal of Immunology
SN - 0022-1767
IS - 9
ER -