Possible involvement of EBV-mediated α-fodrin cleavage for organ-specific autoantigen in Sjogren's syndrome

H. Inoue, Kazuo Tsubota, M. Ono, Y. Kizu, F. Mizuno, K. Takada, K. Yamada, K. Yanagi, Y. Hayashi, I. Saito

Research output: Contribution to journalArticle

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Abstract

A cleavage product of α-fodrin may be an important organ-specific autoantigen in the pathogenesis of Sjogren's syndrome (SS), but the mechanisms of α-fodrin cleavage remain unclear. Since EBV has been implicated in the pathogenesis of SS, we determined whether EBV activation could induce the SS-specific 120-kDa autoantigen α-fodrin. ZEBRA mRNA expression, a marker for activation of the lytic cycle of EBV, was found in the salivary gland tissues from SS patients, but not in those from control individuals. ZEBRA-expressing lymphoid cells were also found in the SS glands in double-stained immunohistochemistry. Furthermore, a significant link between production of Abs against 120-kDa α-fodrin and reactivated EBV Ag was found in sera from patients with SS, but not in those from control individuals. EBV-activated lymphoid cells showed specific α-fodrin cleavage to the expected 120-kDa fragments in vitro. Pretreatment with caspase inhibitors inhibited cleavage of α-fodrin. Thus, an increase in apoptotic protease activities induced by EBV reactivation may be involved in the progression of α-fodrin proteolysis in the development of SS.

Original languageEnglish
Pages (from-to)5801-5809
Number of pages9
JournalJournal of Immunology
Volume166
Issue number9
Publication statusPublished - 2001 May 1
Externally publishedYes

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Sjogren's Syndrome
Autoantigens
Human Herpesvirus 4
Lymphocytes
Caspase Inhibitors
fodrin
Salivary Glands
Proteolysis
Peptide Hydrolases
Immunohistochemistry
Messenger RNA
Serum

ASJC Scopus subject areas

  • Immunology

Cite this

Inoue, H., Tsubota, K., Ono, M., Kizu, Y., Mizuno, F., Takada, K., ... Saito, I. (2001). Possible involvement of EBV-mediated α-fodrin cleavage for organ-specific autoantigen in Sjogren's syndrome. Journal of Immunology, 166(9), 5801-5809.

Possible involvement of EBV-mediated α-fodrin cleavage for organ-specific autoantigen in Sjogren's syndrome. / Inoue, H.; Tsubota, Kazuo; Ono, M.; Kizu, Y.; Mizuno, F.; Takada, K.; Yamada, K.; Yanagi, K.; Hayashi, Y.; Saito, I.

In: Journal of Immunology, Vol. 166, No. 9, 01.05.2001, p. 5801-5809.

Research output: Contribution to journalArticle

Inoue, H, Tsubota, K, Ono, M, Kizu, Y, Mizuno, F, Takada, K, Yamada, K, Yanagi, K, Hayashi, Y & Saito, I 2001, 'Possible involvement of EBV-mediated α-fodrin cleavage for organ-specific autoantigen in Sjogren's syndrome', Journal of Immunology, vol. 166, no. 9, pp. 5801-5809.
Inoue, H. ; Tsubota, Kazuo ; Ono, M. ; Kizu, Y. ; Mizuno, F. ; Takada, K. ; Yamada, K. ; Yanagi, K. ; Hayashi, Y. ; Saito, I. / Possible involvement of EBV-mediated α-fodrin cleavage for organ-specific autoantigen in Sjogren's syndrome. In: Journal of Immunology. 2001 ; Vol. 166, No. 9. pp. 5801-5809.
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