Possible participation of outer mitochondrial membrane cytochrome B 5 in steroidogenesis in zona glomerulosa of rat adrenal cortex

Fumiko Mitani, T. Ogishima, Kuniaki Mukai, R. Hoshino, Kenji Watanabe, M. Suematsu

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Outer mitochondrial membrane cytochrome b5 (OMb) originally found in rat liver is an isoform of cytochrome b5 (b5) of the endoplasmic reticulum. In contrast to accumulated data on the physiological roles of b5, functions of OMb have not been well characterized except for its involvement in regeneration of ascorbic acid [i.e., in a semidehydroascorbate reductase (SDAR) system]. By using highly specific antibodies against rat OMb, we found immunohistochemically that OMb in the rat adrenal gland was most abundant in the zona glomerulosa (zG) among the three cortical zones, and the expression level was enhanced on angiotensin II-stimulation. SDAR activity was found in zG and inhibited by anti-OMb antibody. Moreover, the increase in plasma aldosterone concentration under Na+-deficiency was suppressed by limited ascorbic acid (Asc) availability in rat mutants unable to synthesize Asc, while plasma corticosterone concentration was not affected. These data suggest that OMb, present abundantly in zG, participates in aldosterone formation in zG of rat under angiotensin II-stimulation through regeneration of Asc.

Original languageEnglish
Pages (from-to)639-644
Number of pages6
JournalEndocrine Research
Volume30
Issue number4
DOIs
Publication statusPublished - 2004

Fingerprint

Zona Glomerulosa
Adrenal Cortex
Mitochondrial Membranes
Cytochromes
monodehydroascorbate reductase (NADH)
Ascorbic Acid
Cytochromes b5
Aldosterone
Angiotensin II
Regeneration
Adrenal Glands
Corticosterone
Endoplasmic Reticulum
Anti-Idiotypic Antibodies
Protein Isoforms
Antibodies
Liver

Keywords

  • Cytochrome b
  • Steroidogenesis
  • Zona glomerulosa

ASJC Scopus subject areas

  • Endocrinology

Cite this

Possible participation of outer mitochondrial membrane cytochrome B 5 in steroidogenesis in zona glomerulosa of rat adrenal cortex. / Mitani, Fumiko; Ogishima, T.; Mukai, Kuniaki; Hoshino, R.; Watanabe, Kenji; Suematsu, M.

In: Endocrine Research, Vol. 30, No. 4, 2004, p. 639-644.

Research output: Contribution to journalArticle

Mitani, Fumiko ; Ogishima, T. ; Mukai, Kuniaki ; Hoshino, R. ; Watanabe, Kenji ; Suematsu, M. / Possible participation of outer mitochondrial membrane cytochrome B 5 in steroidogenesis in zona glomerulosa of rat adrenal cortex. In: Endocrine Research. 2004 ; Vol. 30, No. 4. pp. 639-644.
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AU - Watanabe, Kenji

AU - Suematsu, M.

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