Post-ischemic hypothermia delayed neutrophil accumulation and microglial activation following transient focal ischemia in rats

Joji Inamasu, Sadao Suga, Shuzo Sato, Takashi Horiguchi, Kazunori Akaji, Keita Mayanagi, Takeshi Kawase

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Following ischemia, inflammation has been demonstrated to be involved in the progression of the tissue damage. Intra-ischemic hypothermia has been shown to attenuate the adverse activities of neutrophils and microglia. We investigated whether neutrophil accumulation and/or microglial activation is attenuated in post-ischemic hypothermia following transient focal ischemia in rats. After 1 h of ischemia, the neutrophil accumulation and the microglial activation was evaluated immunohistochemically. Percent infarct area was compared at 1, 2, 3, 5, and 7 days after ischemia/reperfusion. In hypothermia, the neutrophil accumulation was delayed but not attenuated. In normothermia, the accumulation reached the peak at 2 days after ischemia. The peak shifted to 3 days in hypothermia. Similarly, the microglial activation was delayed in hypothermia. Comparison of the infarct area showed significant protection by hypothermia at 1 and 2 days after reperfusion. However, hypothermia failed to show significant protection after 3 days and later. These results show that the delayed neutrophil accumulation and the microglial activation can be responsible for the loss of persistent protection in post-ischemic hypothermia. (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)66-74
Number of pages9
JournalJournal of Neuroimmunology
Issue number2
Publication statusPublished - 2000 Sep 22



  • Focal ischemia
  • Microglia
  • Neutrophil
  • Post-ischemic hypothermia

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Neurology
  • Clinical Neurology

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