Post-translational regulation of type I collagen synthesis by heparin in vascular smooth muscle cells

Shingo Tajima, Hiroshi Wachi, Makoto Takehan

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Heparin is a potent inhibitor of smooth muscle cell proliferation. It reduced the level of type I collagen by one-half in culture medium of smooth muscle cells, whereas it increased the type I collagen expression 4-fold in the cell layer compartment under the conditions where it inhibited cell proliferation. No significant change in the level of prolyl hydroxylation in the newly synthesized type I procollagen or steady-state levels of pro α1 (I) and pro α2(I) collagen mRNAs was detected after heparin treatment. Short-pulse and pulse-chase experiments revealed that heparin inhibited the secretion of type I procollagen and stimulated the accumulation of type I collagen in the extracellular space. These results suggest that heparin could modulate type I collagen expression at a posttranslational level. The modulation appeared to be closely related with its inhibitory effect on cell proliferation via two different mechanisms: the inhibition of the secretion of type I procollagen and stabilization of type I collagen in the cell layer. Heparin thus appears to be a multifunc tional modulator for cell proliferation and the expression of type I collagen in vascular smooth muscle cells.

Original languageEnglish
Pages (from-to)353-358
Number of pages6
JournalJournal of biochemistry
Volume117
Issue number2
DOIs
Publication statusPublished - 1995 Feb 1

Keywords

  • Collagen
  • Heparin
  • Smooth muscle cell

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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