Post-translational regulation of type I collagen synthesis by heparin in vascular smooth muscle cells

Shingo Tajima, Hiroshi Wachi, Makoto Takehan

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Heparin is a potent inhibitor of smooth muscle cell proliferation. It reduced the level of type I collagen by one-half in culture medium of smooth muscle cells, whereas it increased the type I collagen expression 4-fold in the cell layer compartment under the conditions where it inhibited cell proliferation. No significant change in the level of prolyl hydroxylation in the newly synthesized type I procollagen or steady-state levels of pro α1 (I) and pro α2(I) collagen mRNAs was detected after heparin treatment. Short-pulse and pulse-chase experiments revealed that heparin inhibited the secretion of type I procollagen and stimulated the accumulation of type I collagen in the extracellular space. These results suggest that heparin could modulate type I collagen expression at a posttranslational level. The modulation appeared to be closely related with its inhibitory effect on cell proliferation via two different mechanisms: the inhibition of the secretion of type I procollagen and stabilization of type I collagen in the cell layer. Heparin thus appears to be a multifunc tional modulator for cell proliferation and the expression of type I collagen in vascular smooth muscle cells.

Original languageEnglish
Pages (from-to)353-358
Number of pages6
JournalJournal of Biochemistry
Volume117
Issue number2
DOIs
Publication statusPublished - 1995 Feb

Fingerprint

Vascular Smooth muscle
Smooth muscle Cells
Heparin
Collagen
Collagen Type I
Vascular Smooth Muscle
Smooth Muscle Myocytes
Muscle
Cells
Synthesis
Cell Proliferation
Cell proliferation
Secretion
Hydroxylation
Short Pulse
Cell
Modulator
Proliferation
Cell culture
Messenger RNA

Keywords

  • Collagen
  • Heparin
  • Smooth muscle cell

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Radiology Nuclear Medicine and imaging
  • Molecular Biology
  • Biochemistry

Cite this

Post-translational regulation of type I collagen synthesis by heparin in vascular smooth muscle cells. / Tajima, Shingo; Wachi, Hiroshi; Takehan, Makoto.

In: Journal of Biochemistry, Vol. 117, No. 2, 02.1995, p. 353-358.

Research output: Contribution to journalArticle

Tajima, Shingo ; Wachi, Hiroshi ; Takehan, Makoto. / Post-translational regulation of type I collagen synthesis by heparin in vascular smooth muscle cells. In: Journal of Biochemistry. 1995 ; Vol. 117, No. 2. pp. 353-358.
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