Postischemic hypothermia attenuates apoptotic cell death in transient focal ischemia in rats.

J. Inamasu, S. Suga, S. Sato, Takashi Horiguchi, K. Akaji, K. Mayanagi, T. Kawase

Research output: Contribution to journalArticle

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Abstract

Hypothermia confers potent neuroprotection against ischemic injury. Attenuation of apoptosis by hypothermia can be one of the responsible mechanisms. In this study, in situ DNA nick-end labeling (TUNEL) and immunostaining of Bax protein were performed to evaluate the effect of postischemic hypothermia on apoptotic cell death, employing rodent transient focal ischemia. Animals received 1 hour of transient focal ischemia. Brain temperature was maintained at 37.5 +/- 0.5 degrees C during ischemia. Immediately after reperfusion, animals were assigned to either a normothermic or hypothermic group. In hypothermia, animals were cooled and brain temperature was lowered to 34.5 +/- 1.0 degrees C. Prolonged hypothermia was maintained for 16 hours and animals rewarmed. In both groups, TUNEL and immunostaining of Bax was performed. In normothermia, the number of TUNEL positive cells reached the peak at 2 days after ischemia and decreased gradually. In hypothermia, the peak was shifted to 3 days after ischemia. The number of TUNEL positive cells in hypothermia was persistently below that of normothermia. Similarly, in hypothermia, immunostaining of Bax showed attenuated immunoreactivity compared with that in normothermia. In conclusion, postischemic hypothermia reduced both the number of TUNEL positive cells and immunoreactivity of Bax, which may be one of the responsible mechanisms with which hypothermia exerts neuroprotection.

Original languageEnglish
Pages (from-to)525-527
Number of pages3
JournalActa Neurochirurgica, Supplement
Volume76
Publication statusPublished - 2000

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Hypothermia
Cell Death
Ischemia
In Situ Nick-End Labeling
Single-Stranded DNA Breaks
bcl-2-Associated X Protein
Temperature
Brain
Reperfusion
Rodentia
Apoptosis
Wounds and Injuries

ASJC Scopus subject areas

  • Medicine(all)
  • Clinical Neurology

Cite this

Inamasu, J., Suga, S., Sato, S., Horiguchi, T., Akaji, K., Mayanagi, K., & Kawase, T. (2000). Postischemic hypothermia attenuates apoptotic cell death in transient focal ischemia in rats. Acta Neurochirurgica, Supplement, 76, 525-527.

Postischemic hypothermia attenuates apoptotic cell death in transient focal ischemia in rats. / Inamasu, J.; Suga, S.; Sato, S.; Horiguchi, Takashi; Akaji, K.; Mayanagi, K.; Kawase, T.

In: Acta Neurochirurgica, Supplement, Vol. 76, 2000, p. 525-527.

Research output: Contribution to journalArticle

Inamasu, J, Suga, S, Sato, S, Horiguchi, T, Akaji, K, Mayanagi, K & Kawase, T 2000, 'Postischemic hypothermia attenuates apoptotic cell death in transient focal ischemia in rats.', Acta Neurochirurgica, Supplement, vol. 76, pp. 525-527.
Inamasu, J. ; Suga, S. ; Sato, S. ; Horiguchi, Takashi ; Akaji, K. ; Mayanagi, K. ; Kawase, T. / Postischemic hypothermia attenuates apoptotic cell death in transient focal ischemia in rats. In: Acta Neurochirurgica, Supplement. 2000 ; Vol. 76. pp. 525-527.
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