Postsynaptic Expression of a New Calcium Pathway in Hippocampal CA3 Neurons and its Influence on Mossy Fiber Long-Term Potentiation

Wataru Kakegawa, Nobuaki Yamada, Masae Iino, Kimihiko Kameyama, Tatsuya Umeda, Keisuke Tsuzuki, Seiji Ozawa

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Long-term potentiation (LTP) in the CA1 region of the hippocampus is induced by postsynaptic Ca2+ influx via NMDA receptors (NMDARs). However, this synaptic plasticity occurs independently of NMDARs when Ca 2+-permeable AMPA receptors (AMPARs) are expressed at postsynaptic sites using various genetic techniques, indicating that an increase in Ca 2+ level at critical postsynaptic sites, regardless of its entry pathway, triggers the induction of LTP at CA1 synapses. In contrast, NMDARs are sparsely distributed on mossy fiber (MF) synapses in CA3 hippocampal neurons, and most evidence favors the presynaptic mechanism for LTP induction, although some reports suggested a postsynaptic mechanism. In this study, we examined whether Ca2+ influx through the newly produced postsynaptic receptors during high-frequency stimulation affects the induction of MF LTR For this purpose, we expressed Ca2+-permeable AMPARs in CA3 pyramidal neurons by Sindbis viral-mediated gene transfer of the unedited form of the glutamate receptor 2 (GluR2Q) subunit, as a new pathway for postsynaptic Ca 2+ entry, in rat hippocampal organotypic cultures. Virally expressed myc-tagged GluR2Q was detected at the complex spines known as the thorny excrescences, which serve as postsynaptic targets for MF synaptic input, on the proximal apical dendrites of CA3 pyramidal cells. Furthermore, endogenous Ca2+-impermeable AMPARs at MF synapses were converted into Ca 2+-permeable receptors by GluR2Q expression. However, the postsynaptic expression of Ca2+-permeable AMPARs had no significant influence on the two types of MF LTP induced by different stimulus protocols. These results supported the notion that MF LTP is independent of postsynaptic Ca2+.

Original languageEnglish
Pages (from-to)4312-4320
Number of pages9
JournalJournal of Neuroscience
Volume22
Issue number11
Publication statusPublished - 2002 Jun 1
Externally publishedYes

Fingerprint

Long-Term Potentiation
AMPA Receptors
Calcium
Neurons
N-Methyl-D-Aspartate Receptors
Synapses
Pyramidal Cells
Genetic Techniques
Neuronal Plasticity
Viral Genes
Glutamate Receptors
Dendrites
Hippocampus
Spine

Keywords

  • Ca influx
  • CA3
  • Ca-permeable AMPA receptors
  • GluR2
  • High-frequency stimulation
  • Hippocampus
  • Mossy fiber LTP
  • Sindbis viral vector

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Postsynaptic Expression of a New Calcium Pathway in Hippocampal CA3 Neurons and its Influence on Mossy Fiber Long-Term Potentiation. / Kakegawa, Wataru; Yamada, Nobuaki; Iino, Masae; Kameyama, Kimihiko; Umeda, Tatsuya; Tsuzuki, Keisuke; Ozawa, Seiji.

In: Journal of Neuroscience, Vol. 22, No. 11, 01.06.2002, p. 4312-4320.

Research output: Contribution to journalArticle

Kakegawa, W, Yamada, N, Iino, M, Kameyama, K, Umeda, T, Tsuzuki, K & Ozawa, S 2002, 'Postsynaptic Expression of a New Calcium Pathway in Hippocampal CA3 Neurons and its Influence on Mossy Fiber Long-Term Potentiation', Journal of Neuroscience, vol. 22, no. 11, pp. 4312-4320.
Kakegawa, Wataru ; Yamada, Nobuaki ; Iino, Masae ; Kameyama, Kimihiko ; Umeda, Tatsuya ; Tsuzuki, Keisuke ; Ozawa, Seiji. / Postsynaptic Expression of a New Calcium Pathway in Hippocampal CA3 Neurons and its Influence on Mossy Fiber Long-Term Potentiation. In: Journal of Neuroscience. 2002 ; Vol. 22, No. 11. pp. 4312-4320.
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