Postsynaptic expression of homeostatic plasticity at neocortical synapses

Corette J. Wierenga, Keiji Ibata, Gina G. Turrigiano

Research output: Contribution to journalArticle

216 Citations (Scopus)

Abstract

Synaptic scaling is a form of homeostatic plasticity that scales synaptic strengths up or down to compensate for prolonged changes in activity. It has been controversial whether this plasticity is expressed presynaptically, postsynaptically, or both. Here we describe in detail the homeostatic changes that take place at excitatory synapses in visual cortical cultures after 1 or 2 d of activity blockade. After 7-10 d in vitro, activity blockade significantly increased postsynaptic accumulation of synaptic AMPA receptors via proportional increases in glutamate receptor 1 (GluR1) and GluR2. Time-lapse imaging of enhanced green fluorescent protein-tagged AMPA receptors revealed that receptor accumulation increased progressively over 2 d of activity blockade and affected the entire population of imaged synapses. The strength of synaptic connections between pyramidal neurons was more than doubled after activity blockade without affecting short-term depression or the coefficient of variation of the postsynaptic responses. Furthermore, uptake of the fluorescent styryl dye FM1-43 (N-(3-triethylammoniumpropyl)-4-[4-(dibutylamino)styryl] pyridinium dibromide) by presynaptic terminals was not different at control and activity-blocked synapses. In addition to the increased accumulation of postsynaptic AMPA receptors, boosting of dendritic AMPA currents by sodium channels was increased by activity blockade. These data indicate that, at young neocortical synapses, synaptic scaling has a predominantly postsynaptic locus and functions as a gain control mechanism to regulate neuronal activity without affecting the dynamics of synaptic transmission.

Original languageEnglish
Pages (from-to)2895-2905
Number of pages11
JournalJournal of Neuroscience
Volume25
Issue number11
DOIs
Publication statusPublished - 2005 Mar 16
Externally publishedYes

Fingerprint

Synapses
AMPA Receptors
Time-Lapse Imaging
alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
Neurotransmitter Receptor
Neuronal Plasticity
Sodium Channels
Pyramidal Cells
Presynaptic Terminals
Glutamate Receptors
Fluorescent Dyes
Synaptic Transmission
Population

Keywords

  • AMPA receptors
  • FM1-43
  • Homeostasis
  • Short-term plasticity
  • Sodium currents
  • Synaptic plasticity
  • Time-lapse imaging

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Postsynaptic expression of homeostatic plasticity at neocortical synapses. / Wierenga, Corette J.; Ibata, Keiji; Turrigiano, Gina G.

In: Journal of Neuroscience, Vol. 25, No. 11, 16.03.2005, p. 2895-2905.

Research output: Contribution to journalArticle

Wierenga, Corette J. ; Ibata, Keiji ; Turrigiano, Gina G. / Postsynaptic expression of homeostatic plasticity at neocortical synapses. In: Journal of Neuroscience. 2005 ; Vol. 25, No. 11. pp. 2895-2905.
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