Potent inhibition of dinuclear zinc(II) peptidase, an aminopeptidase from Aeromonas proteolytica, by 8-quinolinol derivatives: Inhibitor design based on Zn 2+ fluorophores, kinetic, and X-ray crystallographic study

Kengo Hanaya, Miho Suetsugu, Shinya Saijo, Ichiro Yamato, Shin Aoki

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The selective inhibition of an aminopeptidase from Aeromonas proteolytica (AAP), a dinuclear Zn2+ hydrolase, by 8-quinolinol (8-hydroxyquinoline, 8-HQ) derivatives is reported. We previously reported on the preparation of 8-HQ-pendant cyclens as Zn 2+ fluorophores (cyclen is 1,4,7,10-tetraazacyclododecane), in which the nitrogen and phenolate of the 8-HQ units (as well as the four nitrogens of cyclen) bind to Zn 2+ in a bidentate manner to form very stable Zn 2+ complexes at neutral pH (Kd = 8-50 fM at pH 7.4). On the basis of this finding, it was hypothesized that 8-HQ derivatives have the potential to function as specific inhibitors of Zn2+ enzymes, especially dinuclear Zn 2+ hydrolases. Assays of 8-HQ derivatives as inhibitors were performed against commercially available dinuclear Zn 2+ enzymes such as AAP and alkaline phosphatase. 8-HQ and the 5-substituted 8-HQ derivatives were found to be competitive inhibitors of AAP with inhibition constants of 0.16-29 lM at pH 8.0. The nitrogen at the 1-position and the hydroxide at the 8-position of 8-HQ were found to be essential for the inhibition of AAP. Fluorescence titrations of these drugs with AAP and an X-ray crystal structure analysis of an AAP-8-HQ complex (1.3-Å resolution) confirmed that 8-HQ binds to AAP in the ''Pyr-out'' mode, in which the hydroxide anion of 8-HQ bridges two Zn2+ ions (Zn1 and Zn2) in the active site of AAP and the nitrogen atom of 8-HQ coordinates to Zn1 (Protein Data Bank code 3VH9).

Original languageEnglish
Pages (from-to)517-529
Number of pages13
JournalJournal of Biological Inorganic Chemistry
Volume17
Issue number4
DOIs
Publication statusPublished - 2012 Apr
Externally publishedYes

Fingerprint

Oxyquinoline
Fluorophores
Zinc
Peptide Hydrolases
X-Rays
Aeromonas
Derivatives
X rays
Kinetics
Nitrogen
Hydrolases
bacterial leucyl aminopeptidase
Enzyme Inhibitors
Enzymes
Titration
Anions
Alkaline Phosphatase

Keywords

  • 8-Quinolinol
  • Aminopeptidase
  • Dinuclear Zn2+ hydrolases
  • Inhibitor

ASJC Scopus subject areas

  • Biochemistry
  • Inorganic Chemistry

Cite this

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title = "Potent inhibition of dinuclear zinc(II) peptidase, an aminopeptidase from Aeromonas proteolytica, by 8-quinolinol derivatives: Inhibitor design based on Zn 2+ fluorophores, kinetic, and X-ray crystallographic study",
abstract = "The selective inhibition of an aminopeptidase from Aeromonas proteolytica (AAP), a dinuclear Zn2+ hydrolase, by 8-quinolinol (8-hydroxyquinoline, 8-HQ) derivatives is reported. We previously reported on the preparation of 8-HQ-pendant cyclens as Zn 2+ fluorophores (cyclen is 1,4,7,10-tetraazacyclododecane), in which the nitrogen and phenolate of the 8-HQ units (as well as the four nitrogens of cyclen) bind to Zn 2+ in a bidentate manner to form very stable Zn 2+ complexes at neutral pH (Kd = 8-50 fM at pH 7.4). On the basis of this finding, it was hypothesized that 8-HQ derivatives have the potential to function as specific inhibitors of Zn2+ enzymes, especially dinuclear Zn 2+ hydrolases. Assays of 8-HQ derivatives as inhibitors were performed against commercially available dinuclear Zn 2+ enzymes such as AAP and alkaline phosphatase. 8-HQ and the 5-substituted 8-HQ derivatives were found to be competitive inhibitors of AAP with inhibition constants of 0.16-29 lM at pH 8.0. The nitrogen at the 1-position and the hydroxide at the 8-position of 8-HQ were found to be essential for the inhibition of AAP. Fluorescence titrations of these drugs with AAP and an X-ray crystal structure analysis of an AAP-8-HQ complex (1.3-{\AA} resolution) confirmed that 8-HQ binds to AAP in the ''Pyr-out'' mode, in which the hydroxide anion of 8-HQ bridges two Zn2+ ions (Zn1 and Zn2) in the active site of AAP and the nitrogen atom of 8-HQ coordinates to Zn1 (Protein Data Bank code 3VH9).",
keywords = "8-Quinolinol, Aminopeptidase, Dinuclear Zn2+ hydrolases, Inhibitor",
author = "Kengo Hanaya and Miho Suetsugu and Shinya Saijo and Ichiro Yamato and Shin Aoki",
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T1 - Potent inhibition of dinuclear zinc(II) peptidase, an aminopeptidase from Aeromonas proteolytica, by 8-quinolinol derivatives

T2 - Inhibitor design based on Zn 2+ fluorophores, kinetic, and X-ray crystallographic study

AU - Hanaya, Kengo

AU - Suetsugu, Miho

AU - Saijo, Shinya

AU - Yamato, Ichiro

AU - Aoki, Shin

PY - 2012/4

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AB - The selective inhibition of an aminopeptidase from Aeromonas proteolytica (AAP), a dinuclear Zn2+ hydrolase, by 8-quinolinol (8-hydroxyquinoline, 8-HQ) derivatives is reported. We previously reported on the preparation of 8-HQ-pendant cyclens as Zn 2+ fluorophores (cyclen is 1,4,7,10-tetraazacyclododecane), in which the nitrogen and phenolate of the 8-HQ units (as well as the four nitrogens of cyclen) bind to Zn 2+ in a bidentate manner to form very stable Zn 2+ complexes at neutral pH (Kd = 8-50 fM at pH 7.4). On the basis of this finding, it was hypothesized that 8-HQ derivatives have the potential to function as specific inhibitors of Zn2+ enzymes, especially dinuclear Zn 2+ hydrolases. Assays of 8-HQ derivatives as inhibitors were performed against commercially available dinuclear Zn 2+ enzymes such as AAP and alkaline phosphatase. 8-HQ and the 5-substituted 8-HQ derivatives were found to be competitive inhibitors of AAP with inhibition constants of 0.16-29 lM at pH 8.0. The nitrogen at the 1-position and the hydroxide at the 8-position of 8-HQ were found to be essential for the inhibition of AAP. Fluorescence titrations of these drugs with AAP and an X-ray crystal structure analysis of an AAP-8-HQ complex (1.3-Å resolution) confirmed that 8-HQ binds to AAP in the ''Pyr-out'' mode, in which the hydroxide anion of 8-HQ bridges two Zn2+ ions (Zn1 and Zn2) in the active site of AAP and the nitrogen atom of 8-HQ coordinates to Zn1 (Protein Data Bank code 3VH9).

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