Potential biomarker enhancing the activity of tuberculosis, hsa-miR-346

Shunsuke Uno, Tomoyasu Nishimura, Kazumi Nishio, Asami Kohsaka, Eiko Tamizu, Yasushi Nakano, Junko Kagyo, Yukiko Nakajima, Ryosuke Arai, Hanako Hasegawa, Kenichi Arakawa, Shoko Kashimura, Ryota Ishii, Naoki Miyazaki, Yoshifumi Uwamino, Naoki Hasegawa

Research output: Contribution to journalArticlepeer-review

Abstract

Objectives: To determine the usefulness of hsa-miR-346, a potential biomarker enhancing the activity of non-tuberculous mycobacterial diseases, as a biomarker of tuberculosis activity. Methods: We investigated whether hsa-miR-346 is secreted by human macrophages infected with Mycobacterium tuberculosis (M. tuberculosis) in an in vitro study. In addition, a cross-sectional study was conducted first to evaluate whether serum hsa-miR-346 is elevated in patients with tuberculosis compared with that in healthy individuals. Second, we conducted a retrospective study to evaluate whether anti-tuberculosis treatment reduces serum hsa-miR-346 levels. Results: Log hsa-miR-346 levels were significantly elevated in the supernatant of human macrophages infected with M. tuberculosis in a dose-dependent manner. The mean serum log hsa-miR-346 levels were −15.48 (−15.76 to −15.21) in patients with tuberculosis and −16.12 (−16.29 to −15.95) in healthy volunteers, which significantly differed. In addition, hsa-miR-346 significantly decreased at 2 months from starting an anti-tuberculosis treatment. Conclusions: We consider hsa-miR-346 as a potential biomarker enhancing the tuberculosis activity.

Original languageEnglish
Article number102101
JournalTuberculosis
Volume129
DOIs
Publication statusPublished - 2021 Jul

Keywords

  • Biomarker
  • Tuberculosis
  • hsa-miR-346
  • micro RNA

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Microbiology (medical)
  • Infectious Diseases

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