Potential functional neural repair with grafted neural stem cells of early embryonic neuroepithelial origin

Koichi Uchida, Toshihiko Momiyama, Hideyuki Okano, Michisuke Yuzaki, Amane Koizumi, Yutaka Mine, Takeshi Kawase

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The fate of grafted neuroepithelial stem cells in the normal mature brain environment was assessed both morphologically and electrophysiologically to confirm their feasibility in the functional repair of damaged neural circuitry. The neuroepithelial stem cells were harvested from the mesencephalic neural plate of transgenic green fluorescence protein-carrying rat embryos, and implanted into the normal adult rat striatum. The short- and long-term differentiation pattern of donor-derived cells was precisely monitored immunohistochemically. The functional abilities of the donor-derived cells and communication between them and the host were investigated using host-rat brain slices incorporating the graft with whole-cell patch-clamp recording. Vigorous differentiation of the neuroepithelial stem cells into mostly neurons was noted in the short-term with positive staining for tyrosine hydroxylase, suggesting that the donor-derived cells were exclusively following their genetically programmed fate, together with gamma-aminobutyric acid (GABA) and glutamate expression. In the long-term, the large number of donor-derived neurons was sustained, but the staining pattern showed expression of dopamine- and adenosine 3′:5′-monophosphate-regulated phosphoprotein 32, suggesting that some neurons were following environmental cues, together with the appearance of some cholinergic neurons. Some donor-derived astrocytes were also seen in the graft. Many action potentials indicating the presence of both dopaminergic and non-dopaminergic patterns could be elicited and recorded in the donor-derived neurons in addition to spontaneous glutamatergic and GABAergic post-synaptic currents which were strongly shown to be of host origin. Neuroepithelial stem cells are therefore an attractive candidate as a source of donor material for intracerebral grafting in functional repair.

Original languageEnglish
Pages (from-to)276-286
Number of pages11
JournalNeuroscience Research
Volume52
Issue number3
DOIs
Publication statusPublished - 2005 Jul

Fingerprint

Neuroepithelial Cells
Neural Stem Cells
Stem Cells
Neurons
Staining and Labeling
Neural Plate
Transplants
Cholinergic Neurons
Aptitude
Phosphoproteins
Brain
Tyrosine 3-Monooxygenase
Cell Communication
Astrocytes
Adenosine
gamma-Aminobutyric Acid
Action Potentials
Cues
Glutamic Acid
Dopamine

Keywords

  • Neural stem cell
  • Neural transplantation
  • Neuroepithelium
  • Patch clamp
  • Regenerative medicine
  • Synapse

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Potential functional neural repair with grafted neural stem cells of early embryonic neuroepithelial origin. / Uchida, Koichi; Momiyama, Toshihiko; Okano, Hideyuki; Yuzaki, Michisuke; Koizumi, Amane; Mine, Yutaka; Kawase, Takeshi.

In: Neuroscience Research, Vol. 52, No. 3, 07.2005, p. 276-286.

Research output: Contribution to journalArticle

Uchida, Koichi ; Momiyama, Toshihiko ; Okano, Hideyuki ; Yuzaki, Michisuke ; Koizumi, Amane ; Mine, Yutaka ; Kawase, Takeshi. / Potential functional neural repair with grafted neural stem cells of early embryonic neuroepithelial origin. In: Neuroscience Research. 2005 ; Vol. 52, No. 3. pp. 276-286.
@article{91ccf856cd8c43a6844aa2ac6214bd01,
title = "Potential functional neural repair with grafted neural stem cells of early embryonic neuroepithelial origin",
abstract = "The fate of grafted neuroepithelial stem cells in the normal mature brain environment was assessed both morphologically and electrophysiologically to confirm their feasibility in the functional repair of damaged neural circuitry. The neuroepithelial stem cells were harvested from the mesencephalic neural plate of transgenic green fluorescence protein-carrying rat embryos, and implanted into the normal adult rat striatum. The short- and long-term differentiation pattern of donor-derived cells was precisely monitored immunohistochemically. The functional abilities of the donor-derived cells and communication between them and the host were investigated using host-rat brain slices incorporating the graft with whole-cell patch-clamp recording. Vigorous differentiation of the neuroepithelial stem cells into mostly neurons was noted in the short-term with positive staining for tyrosine hydroxylase, suggesting that the donor-derived cells were exclusively following their genetically programmed fate, together with gamma-aminobutyric acid (GABA) and glutamate expression. In the long-term, the large number of donor-derived neurons was sustained, but the staining pattern showed expression of dopamine- and adenosine 3′:5′-monophosphate-regulated phosphoprotein 32, suggesting that some neurons were following environmental cues, together with the appearance of some cholinergic neurons. Some donor-derived astrocytes were also seen in the graft. Many action potentials indicating the presence of both dopaminergic and non-dopaminergic patterns could be elicited and recorded in the donor-derived neurons in addition to spontaneous glutamatergic and GABAergic post-synaptic currents which were strongly shown to be of host origin. Neuroepithelial stem cells are therefore an attractive candidate as a source of donor material for intracerebral grafting in functional repair.",
keywords = "Neural stem cell, Neural transplantation, Neuroepithelium, Patch clamp, Regenerative medicine, Synapse",
author = "Koichi Uchida and Toshihiko Momiyama and Hideyuki Okano and Michisuke Yuzaki and Amane Koizumi and Yutaka Mine and Takeshi Kawase",
year = "2005",
month = "7",
doi = "10.1016/j.neures.2005.03.015",
language = "English",
volume = "52",
pages = "276--286",
journal = "Neuroscience Research",
issn = "0168-0102",
publisher = "Elsevier Ireland Ltd",
number = "3",

}

TY - JOUR

T1 - Potential functional neural repair with grafted neural stem cells of early embryonic neuroepithelial origin

AU - Uchida, Koichi

AU - Momiyama, Toshihiko

AU - Okano, Hideyuki

AU - Yuzaki, Michisuke

AU - Koizumi, Amane

AU - Mine, Yutaka

AU - Kawase, Takeshi

PY - 2005/7

Y1 - 2005/7

N2 - The fate of grafted neuroepithelial stem cells in the normal mature brain environment was assessed both morphologically and electrophysiologically to confirm their feasibility in the functional repair of damaged neural circuitry. The neuroepithelial stem cells were harvested from the mesencephalic neural plate of transgenic green fluorescence protein-carrying rat embryos, and implanted into the normal adult rat striatum. The short- and long-term differentiation pattern of donor-derived cells was precisely monitored immunohistochemically. The functional abilities of the donor-derived cells and communication between them and the host were investigated using host-rat brain slices incorporating the graft with whole-cell patch-clamp recording. Vigorous differentiation of the neuroepithelial stem cells into mostly neurons was noted in the short-term with positive staining for tyrosine hydroxylase, suggesting that the donor-derived cells were exclusively following their genetically programmed fate, together with gamma-aminobutyric acid (GABA) and glutamate expression. In the long-term, the large number of donor-derived neurons was sustained, but the staining pattern showed expression of dopamine- and adenosine 3′:5′-monophosphate-regulated phosphoprotein 32, suggesting that some neurons were following environmental cues, together with the appearance of some cholinergic neurons. Some donor-derived astrocytes were also seen in the graft. Many action potentials indicating the presence of both dopaminergic and non-dopaminergic patterns could be elicited and recorded in the donor-derived neurons in addition to spontaneous glutamatergic and GABAergic post-synaptic currents which were strongly shown to be of host origin. Neuroepithelial stem cells are therefore an attractive candidate as a source of donor material for intracerebral grafting in functional repair.

AB - The fate of grafted neuroepithelial stem cells in the normal mature brain environment was assessed both morphologically and electrophysiologically to confirm their feasibility in the functional repair of damaged neural circuitry. The neuroepithelial stem cells were harvested from the mesencephalic neural plate of transgenic green fluorescence protein-carrying rat embryos, and implanted into the normal adult rat striatum. The short- and long-term differentiation pattern of donor-derived cells was precisely monitored immunohistochemically. The functional abilities of the donor-derived cells and communication between them and the host were investigated using host-rat brain slices incorporating the graft with whole-cell patch-clamp recording. Vigorous differentiation of the neuroepithelial stem cells into mostly neurons was noted in the short-term with positive staining for tyrosine hydroxylase, suggesting that the donor-derived cells were exclusively following their genetically programmed fate, together with gamma-aminobutyric acid (GABA) and glutamate expression. In the long-term, the large number of donor-derived neurons was sustained, but the staining pattern showed expression of dopamine- and adenosine 3′:5′-monophosphate-regulated phosphoprotein 32, suggesting that some neurons were following environmental cues, together with the appearance of some cholinergic neurons. Some donor-derived astrocytes were also seen in the graft. Many action potentials indicating the presence of both dopaminergic and non-dopaminergic patterns could be elicited and recorded in the donor-derived neurons in addition to spontaneous glutamatergic and GABAergic post-synaptic currents which were strongly shown to be of host origin. Neuroepithelial stem cells are therefore an attractive candidate as a source of donor material for intracerebral grafting in functional repair.

KW - Neural stem cell

KW - Neural transplantation

KW - Neuroepithelium

KW - Patch clamp

KW - Regenerative medicine

KW - Synapse

UR - http://www.scopus.com/inward/record.url?scp=20144383631&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=20144383631&partnerID=8YFLogxK

U2 - 10.1016/j.neures.2005.03.015

DO - 10.1016/j.neures.2005.03.015

M3 - Article

C2 - 15927727

AN - SCOPUS:20144383631

VL - 52

SP - 276

EP - 286

JO - Neuroscience Research

JF - Neuroscience Research

SN - 0168-0102

IS - 3

ER -