Powdery mildew-infection changes bacterial community composition in the phyllosphere

Wataru Suda, Asami Nagasaki, Masahiro Shishido

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

gate changes in bacterial communities associated with a fungal foliar disease, epiphytic bacteria from powdery mildew-infected and uninfected leaves of cucumber and Japanese spindle were analyzed using both culture- dependent and -independent methods. Dilution plate counting suggested that powdery mildew-infected leaves likely accommodated larger populations of phyllosphere bacteria than uninfected leaves. Community-level physiological profiles (CLPP) also indicated that functional diversity, richness, and evenness of bacterial communities were significantly greater in the phyllosphere of powdery mildew-infected leaves. Genotype diversity and richness based on band patterns of denaturing gradient gel electrophoresis (DGGE) of the phyllosphere bacterial community were greater for leaves infected by powdery mildew. A principle component analysis of CLPP and DGGE patterns revealed a clear difference between infected and uninfected leaves of both plant species. These results suggest that powdery mildew-infection results in larger bacterial populations, and greater diversity and richness, and also changes the structure of the phyllosphere bacterial community. Furthermore, DNA sequences of the DGGE bands that showed greater intensity in the infected than uninfected leaves, differed between cucumber and Japanese spindle. This suggests that specific bacteria are associated with the plant species accompanying this fungal infection.

Original languageEnglish
Pages (from-to)217-223
Number of pages7
JournalMicrobes and Environments
Volume24
Issue number3
DOIs
Publication statusPublished - 2009
Externally publishedYes

Fingerprint

phyllosphere
powdery mildew
bacterial communities
community composition
infection
denaturing gradient gel electrophoresis
leaves
electrokinesis
gel
cucumbers
bacterium
bacteria
fungal disease
foliar diseases
functional diversity
principal component analysis
dilution
genotype
nucleotide sequences
DNA

Keywords

  • Community-level physiological profile (CLPP)
  • Denaturing gradient gel electrophoresis (DGGE)
  • Epiphytic bacteria
  • Phyllosphere
  • Powdery mildew

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Soil Science
  • Plant Science

Cite this

Powdery mildew-infection changes bacterial community composition in the phyllosphere. / Suda, Wataru; Nagasaki, Asami; Shishido, Masahiro.

In: Microbes and Environments, Vol. 24, No. 3, 2009, p. 217-223.

Research output: Contribution to journalArticle

Suda, Wataru ; Nagasaki, Asami ; Shishido, Masahiro. / Powdery mildew-infection changes bacterial community composition in the phyllosphere. In: Microbes and Environments. 2009 ; Vol. 24, No. 3. pp. 217-223.
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