Practical fluorescence detection of acrolein in human plasma via a two-step tethering approach

Masataka Togashi, Takuya Terai, Hirotatsu Kojima, Kenjiro Hanaoka, Kazuei Igarashi, Yasunobu Hirata, Yasuteru Urano, Tetsuo Nagano

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

Acrolein, a cytotoxic α,β-unsaturated aldehyde and disease biomarker, was determined in plasma by means of a novel tethering strategy using Michael addition of the compound to a fluorescent dye, followed by immobilization of the product on microbeads via the aldehyde moiety. Elevation of blood acrolein was detected in mice treated with an anticancer agent cyclophosphamide, which releases acrolein upon activation. This method should be suitable for high-throughput diagnostic and clinical application.

Original languageEnglish
Pages (from-to)14946-14948
Number of pages3
JournalChemical Communications
Volume50
Issue number95
DOIs
Publication statusPublished - 2014 Nov 4
Externally publishedYes

ASJC Scopus subject areas

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

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