Practical Optimization Method for Dual-Flow Countercurrent Extraction: Purification of Glycyrrhizin

Hideyuki Nishizawa, Sakiko Okimura, Yoshihiro Abe

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)


An optimization method for the dual-flow countercurrent extraction of a mixture, the components of which show a concentration dependency in its distribution ratio, has been developed. As an experimental demonstration, commercial glycyrrhizin (about 90% purity) was purified to 99.8% with high recovery using a two-phase system (1-butanol and 0.1 M NaCl) in the presence of 0.02 M tetrabutylammonium hydroxide, and under the optimum conditions of the present method. To acquire 90.0% recovery of glycyrrhizin in an organic extract, the flow rate of the organic solvent was calculated to be 0.943 ml/ min (aqueous solvent, 1.06 ml/ min). Extraction performed at the derived flow rates obtained 87.2% recovery of glycyrrhizin in the organic extract, showing the propriety of the optimization method.

Original languageEnglish
Pages (from-to)367-374
Number of pages8
Journalanalytical sciences
Issue number3
Publication statusPublished - 1992
Externally publishedYes


  • Purification
  • countercurrent extraction
  • glycyrrhizic acid
  • glycyrrhizin
  • liquid particle extraction

ASJC Scopus subject areas

  • Analytical Chemistry


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