Practical Optimization Method for Dual-Flow Countercurrent Extraction: Purification of Glycyrrhizin

Hideyuki Nishizawa, Sakiko Okimura, Yoshihiro Abe

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

An optimization method for the dual-flow countercurrent extraction of a mixture, the components of which show a concentration dependency in its distribution ratio, has been developed. As an experimental demonstration, commercial glycyrrhizin (about 90% purity) was purified to 99.8% with high recovery using a two-phase system (1-butanol and 0.1 M NaCl) in the presence of 0.02 M tetrabutylammonium hydroxide, and under the optimum conditions of the present method. To acquire 90.0% recovery of glycyrrhizin in an organic extract, the flow rate of the organic solvent was calculated to be 0.943 ml/ min (aqueous solvent, 1.06 ml/ min). Extraction performed at the derived flow rates obtained 87.2% recovery of glycyrrhizin in the organic extract, showing the propriety of the optimization method.

Original languageEnglish
Pages (from-to)367-374
Number of pages8
JournalAnalytical Sciences
Volume8
Issue number3
DOIs
Publication statusPublished - 1992

Fingerprint

Glycyrrhizic Acid
Purification
Recovery
Flow rate
1-Butanol
Organic solvents
Demonstrations

Keywords

  • countercurrent extraction
  • glycyrrhizic acid
  • glycyrrhizin
  • liquid particle extraction
  • Purification

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Practical Optimization Method for Dual-Flow Countercurrent Extraction : Purification of Glycyrrhizin. / Nishizawa, Hideyuki; Okimura, Sakiko; Abe, Yoshihiro.

In: Analytical Sciences, Vol. 8, No. 3, 1992, p. 367-374.

Research output: Contribution to journalArticle

Nishizawa, Hideyuki ; Okimura, Sakiko ; Abe, Yoshihiro. / Practical Optimization Method for Dual-Flow Countercurrent Extraction : Purification of Glycyrrhizin. In: Analytical Sciences. 1992 ; Vol. 8, No. 3. pp. 367-374.
@article{1c1875e662d04d229fac36f954916c8c,
title = "Practical Optimization Method for Dual-Flow Countercurrent Extraction: Purification of Glycyrrhizin",
abstract = "An optimization method for the dual-flow countercurrent extraction of a mixture, the components of which show a concentration dependency in its distribution ratio, has been developed. As an experimental demonstration, commercial glycyrrhizin (about 90{\%} purity) was purified to 99.8{\%} with high recovery using a two-phase system (1-butanol and 0.1 M NaCl) in the presence of 0.02 M tetrabutylammonium hydroxide, and under the optimum conditions of the present method. To acquire 90.0{\%} recovery of glycyrrhizin in an organic extract, the flow rate of the organic solvent was calculated to be 0.943 ml/ min (aqueous solvent, 1.06 ml/ min). Extraction performed at the derived flow rates obtained 87.2{\%} recovery of glycyrrhizin in the organic extract, showing the propriety of the optimization method.",
keywords = "countercurrent extraction, glycyrrhizic acid, glycyrrhizin, liquid particle extraction, Purification",
author = "Hideyuki Nishizawa and Sakiko Okimura and Yoshihiro Abe",
year = "1992",
doi = "10.2116/analsci.8.367",
language = "English",
volume = "8",
pages = "367--374",
journal = "Analytical Sciences",
issn = "0910-6340",
publisher = "Japan Society for Analytical Chemistry",
number = "3",

}

TY - JOUR

T1 - Practical Optimization Method for Dual-Flow Countercurrent Extraction

T2 - Purification of Glycyrrhizin

AU - Nishizawa, Hideyuki

AU - Okimura, Sakiko

AU - Abe, Yoshihiro

PY - 1992

Y1 - 1992

N2 - An optimization method for the dual-flow countercurrent extraction of a mixture, the components of which show a concentration dependency in its distribution ratio, has been developed. As an experimental demonstration, commercial glycyrrhizin (about 90% purity) was purified to 99.8% with high recovery using a two-phase system (1-butanol and 0.1 M NaCl) in the presence of 0.02 M tetrabutylammonium hydroxide, and under the optimum conditions of the present method. To acquire 90.0% recovery of glycyrrhizin in an organic extract, the flow rate of the organic solvent was calculated to be 0.943 ml/ min (aqueous solvent, 1.06 ml/ min). Extraction performed at the derived flow rates obtained 87.2% recovery of glycyrrhizin in the organic extract, showing the propriety of the optimization method.

AB - An optimization method for the dual-flow countercurrent extraction of a mixture, the components of which show a concentration dependency in its distribution ratio, has been developed. As an experimental demonstration, commercial glycyrrhizin (about 90% purity) was purified to 99.8% with high recovery using a two-phase system (1-butanol and 0.1 M NaCl) in the presence of 0.02 M tetrabutylammonium hydroxide, and under the optimum conditions of the present method. To acquire 90.0% recovery of glycyrrhizin in an organic extract, the flow rate of the organic solvent was calculated to be 0.943 ml/ min (aqueous solvent, 1.06 ml/ min). Extraction performed at the derived flow rates obtained 87.2% recovery of glycyrrhizin in the organic extract, showing the propriety of the optimization method.

KW - countercurrent extraction

KW - glycyrrhizic acid

KW - glycyrrhizin

KW - liquid particle extraction

KW - Purification

UR - http://www.scopus.com/inward/record.url?scp=85004246527&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85004246527&partnerID=8YFLogxK

U2 - 10.2116/analsci.8.367

DO - 10.2116/analsci.8.367

M3 - Article

AN - SCOPUS:85004246527

VL - 8

SP - 367

EP - 374

JO - Analytical Sciences

JF - Analytical Sciences

SN - 0910-6340

IS - 3

ER -