Preconditioning with heat shock further improved functional recovery in young adult but not in middle-aged rat hearts

Yukako Honma, Masato Tani, Ken Yamamura, Michiyo Takayama, Hiroshi Hasegawa

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Ischemic preconditioning (PC) improves post-ischemic function, and heat shock (HS) mimics delayed PC in young animals. However, PC is not protective and the consequences of HS are not known in the aging hearts. This report examines the efficacy of HS and its synergy with PC in the middle-aged rat hearts. Hearts from 12- or 50-week-old rats were subjected to PC before 25min ischemia followed by 30min reperfusion 48h after HS. HS induced HS proteins (HSP) in both age groups but that PC and HS translocated PKC-α and -δ only in young rats. The beneficial effects of HS and PC were additive and enhanced protein kinase C (PKC) translocation in young rats. However, neither HS alone nor in combination with PC conferred any functional advantage or accelerated PKC translocation in old rats. Similarly neither HS alone nor in combination with PC restore PC effects in old rats with impaired PKC activation, despite the induction of HSP, indicating that induction of HSP is insufficient for cytoprotection.

Original languageEnglish
Pages (from-to)299-306
Number of pages8
JournalExperimental Gerontology
Volume38
Issue number3
DOIs
Publication statusPublished - 2003 Mar 1

    Fingerprint

Keywords

  • Aging
  • Heart
  • Heat shock
  • Ischemia
  • Ischemic preconditioning
  • Protein kinase C
  • Reperfusion

ASJC Scopus subject areas

  • Biochemistry
  • Ageing
  • Molecular Biology
  • Genetics
  • Endocrinology
  • Cell Biology

Cite this