Prediction of myocardial damage depth induced by extracellular photosensitization reaction using fluorescence measurement in vivo

M. Takahashi, E. Ogawa, T. Nakamura, H. Kawakami, N. Machida, M. Yajima, M. Kurotsu, A. Ito, T. Kimura, Tsunenori Arai

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We experimentally studied the correlation between myocardial damage depth due to the extracellular photosensitization reaction (PR) using talaporfin sodium and fluorescence-fall amount (FA), which is calculated from the measured backscattering fluorescence intensity via a manipulatable 7 Fr. laser catheter during the PR operation in vivo to establish treatment depth predictor for a non-thermal tachyarrhythmia treatment. The PR was performed to left and/or right ventricle in the open-chest canine heart. The laser irradiation of 663±2 nm in wavelength via the laser catheter was operated 15 min after the intravenous administration of talaporfin sodium with concentration of 36.2±8.0 μg/ml in plasma. The irradiation was operated with irradiance of 5, 10, 20 W/cm2, and duration of 5, 10, 20 s. Backscattering fluorescence of 710±2 nm in wavelength was measured via the laser catheter during the PR. The FA was calculated multiplying the irradiation duration by the fluorescence-fall, which is subtraction of the fluorescence intensity at the kickoff and end of the irradiation. The canine heart was extracted 1 week after the PR and HE stained specimen was histologically evaluated. The correlation of the myocardial damage depth and FA was investigated. We found that FA obtained a logarithmic relation to the myocardial damage depth. We think that the FA might be available to predict the PR induced myocardial damage depth for the application of tachyarrhythmia treatment under catheterization in vivo.

Original languageEnglish
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
PublisherSPIE
Volume8926
ISBN (Print)9780819498397
DOIs
Publication statusPublished - 2014
EventPhotonic Therapeutics and Diagnostics X - San Francisco, CA, United States
Duration: 2014 Feb 12014 Feb 2

Other

OtherPhotonic Therapeutics and Diagnostics X
CountryUnited States
CitySan Francisco, CA
Period14/2/114/2/2

Fingerprint

Photosensitivity Disorders
Fluorescence
Catheters
damage
fluorescence
Lasers
predictions
Irradiation
Backscattering
irradiation
Tachycardia
Heart Ventricles
lasers
Sodium
Canidae
backscattering
Wavelength
sodium
catheterization
Laser beam effects

Keywords

  • Fluorescence monitor
  • Myocardium
  • Photosensitization reaction

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Takahashi, M., Ogawa, E., Nakamura, T., Kawakami, H., Machida, N., Yajima, M., ... Arai, T. (2014). Prediction of myocardial damage depth induced by extracellular photosensitization reaction using fluorescence measurement in vivo. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 8926). [89262X] SPIE. https://doi.org/10.1117/12.2036506

Prediction of myocardial damage depth induced by extracellular photosensitization reaction using fluorescence measurement in vivo. / Takahashi, M.; Ogawa, E.; Nakamura, T.; Kawakami, H.; Machida, N.; Yajima, M.; Kurotsu, M.; Ito, A.; Kimura, T.; Arai, Tsunenori.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 8926 SPIE, 2014. 89262X.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Takahashi, M, Ogawa, E, Nakamura, T, Kawakami, H, Machida, N, Yajima, M, Kurotsu, M, Ito, A, Kimura, T & Arai, T 2014, Prediction of myocardial damage depth induced by extracellular photosensitization reaction using fluorescence measurement in vivo. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 8926, 89262X, SPIE, Photonic Therapeutics and Diagnostics X, San Francisco, CA, United States, 14/2/1. https://doi.org/10.1117/12.2036506
Takahashi M, Ogawa E, Nakamura T, Kawakami H, Machida N, Yajima M et al. Prediction of myocardial damage depth induced by extracellular photosensitization reaction using fluorescence measurement in vivo. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 8926. SPIE. 2014. 89262X https://doi.org/10.1117/12.2036506
Takahashi, M. ; Ogawa, E. ; Nakamura, T. ; Kawakami, H. ; Machida, N. ; Yajima, M. ; Kurotsu, M. ; Ito, A. ; Kimura, T. ; Arai, Tsunenori. / Prediction of myocardial damage depth induced by extracellular photosensitization reaction using fluorescence measurement in vivo. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 8926 SPIE, 2014.
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