Preferential activation of CD4+Vβ5.2/5.3+ intestinal intraepithelial lymphocytes in the inflamed lesions of Crohn's disease

Mamoru Watanabe, Atsushi Hayashi, Yasuo Hosoda, Makoto Ohara, Yasushi Iwao, Hiromasa Ishii, Hiromichi Ishikawa, Masanobu Nanno, Toshifumi Hibi

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Abstract

To analyze the nature of intestinal intraepithelial lymphocytes (iIELs) in inflammatory intestinal disease, we established T cell lines of iIELs isolated from endoscopic biopsied ileal and colonic mucosa of Crohn's disease patients. Seven T cell lines from the inflamed terminal ileum of 13 patients, but none of 16 T cell lines from normal terminal ileum, have shown a deviation of T cell receptor variable region gene usage and were enriched in the proportion of CD4+Vβ5.2/5.3+ cells. CD4+Vβ5.2/5.3+ cells in the T cell lines were not increased after stimulation with purified protein derivatives or 65-kDa heat-shock protein but significantly increased after stimulation with staphylococcal enterotoxins C1 and D. Those cells showed increased cytolytic activity against target cells cross-linked by anti-Vβ5.2/5.3 and produced a large amount of interferon-γ These results indicated that CD4+Vβ5.2/5.3+ iIELs were preferentially activated in the inflamed lesions of Crohn's disease and may play a possible role in the triggering and progression of human inflammatory intestinal disease.

Original languageEnglish
Pages (from-to)130-139
Number of pages10
JournalClinical Immunology and Immunopathology
Volume78
Issue number2
DOIs
Publication statusPublished - 1996

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Crohn Disease
Lymphocytes
T-Lymphocytes
Cell Line
Intestinal Diseases
Ileum
Heat-Shock Proteins
T-Cell Antigen Receptor
Interferons
Mucous Membrane
Genes
Proteins

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Pathology and Forensic Medicine

Cite this

Preferential activation of CD4+Vβ5.2/5.3+ intestinal intraepithelial lymphocytes in the inflamed lesions of Crohn's disease. / Watanabe, Mamoru; Hayashi, Atsushi; Hosoda, Yasuo; Ohara, Makoto; Iwao, Yasushi; Ishii, Hiromasa; Ishikawa, Hiromichi; Nanno, Masanobu; Hibi, Toshifumi.

In: Clinical Immunology and Immunopathology, Vol. 78, No. 2, 1996, p. 130-139.

Research output: Contribution to journalArticle

Watanabe, Mamoru ; Hayashi, Atsushi ; Hosoda, Yasuo ; Ohara, Makoto ; Iwao, Yasushi ; Ishii, Hiromasa ; Ishikawa, Hiromichi ; Nanno, Masanobu ; Hibi, Toshifumi. / Preferential activation of CD4+Vβ5.2/5.3+ intestinal intraepithelial lymphocytes in the inflamed lesions of Crohn's disease. In: Clinical Immunology and Immunopathology. 1996 ; Vol. 78, No. 2. pp. 130-139.
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