Abstract
To analyze the nature of intestinal intraepithelial lymphocytes (iIELs) in inflammatory intestinal disease, we established T cell lines of iIELs isolated from endoscopic biopsied ileal and colonic mucosa of Crohn's disease patients. Seven T cell lines from the inflamed terminal ileum of 13 patients, but none of 16 T cell lines from normal terminal ileum, have shown a deviation of T cell receptor variable region gene usage and were enriched in the proportion of CD4+Vβ5.2/5.3+ cells. CD4+Vβ5.2/5.3+ cells in the T cell lines were not increased after stimulation with purified protein derivatives or 65-kDa heat-shock protein but significantly increased after stimulation with staphylococcal enterotoxins C1 and D. Those cells showed increased cytolytic activity against target cells cross-linked by anti-Vβ5.2/5.3 and produced a large amount of interferon-γ These results indicated that CD4+Vβ5.2/5.3+ iIELs were preferentially activated in the inflamed lesions of Crohn's disease and may play a possible role in the triggering and progression of human inflammatory intestinal disease.
| Original language | English |
|---|---|
| Pages (from-to) | 130-139 |
| Number of pages | 10 |
| Journal | Clinical Immunology and Immunopathology |
| Volume | 78 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 1996 |
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ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
- Pathology and Forensic Medicine
Cite this
Preferential activation of CD4+Vβ5.2/5.3+ intestinal intraepithelial lymphocytes in the inflamed lesions of Crohn's disease. / Watanabe, Mamoru; Hayashi, Atsushi; Hosoda, Yasuo; Ohara, Makoto; Iwao, Yasushi; Ishii, Hiromasa; Ishikawa, Hiromichi; Nanno, Masanobu; Hibi, Toshifumi.
In: Clinical Immunology and Immunopathology, Vol. 78, No. 2, 1996, p. 130-139.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Preferential activation of CD4+Vβ5.2/5.3+ intestinal intraepithelial lymphocytes in the inflamed lesions of Crohn's disease
AU - Watanabe, Mamoru
AU - Hayashi, Atsushi
AU - Hosoda, Yasuo
AU - Ohara, Makoto
AU - Iwao, Yasushi
AU - Ishii, Hiromasa
AU - Ishikawa, Hiromichi
AU - Nanno, Masanobu
AU - Hibi, Toshifumi
PY - 1996
Y1 - 1996
N2 - To analyze the nature of intestinal intraepithelial lymphocytes (iIELs) in inflammatory intestinal disease, we established T cell lines of iIELs isolated from endoscopic biopsied ileal and colonic mucosa of Crohn's disease patients. Seven T cell lines from the inflamed terminal ileum of 13 patients, but none of 16 T cell lines from normal terminal ileum, have shown a deviation of T cell receptor variable region gene usage and were enriched in the proportion of CD4+Vβ5.2/5.3+ cells. CD4+Vβ5.2/5.3+ cells in the T cell lines were not increased after stimulation with purified protein derivatives or 65-kDa heat-shock protein but significantly increased after stimulation with staphylococcal enterotoxins C1 and D. Those cells showed increased cytolytic activity against target cells cross-linked by anti-Vβ5.2/5.3 and produced a large amount of interferon-γ These results indicated that CD4+Vβ5.2/5.3+ iIELs were preferentially activated in the inflamed lesions of Crohn's disease and may play a possible role in the triggering and progression of human inflammatory intestinal disease.
AB - To analyze the nature of intestinal intraepithelial lymphocytes (iIELs) in inflammatory intestinal disease, we established T cell lines of iIELs isolated from endoscopic biopsied ileal and colonic mucosa of Crohn's disease patients. Seven T cell lines from the inflamed terminal ileum of 13 patients, but none of 16 T cell lines from normal terminal ileum, have shown a deviation of T cell receptor variable region gene usage and were enriched in the proportion of CD4+Vβ5.2/5.3+ cells. CD4+Vβ5.2/5.3+ cells in the T cell lines were not increased after stimulation with purified protein derivatives or 65-kDa heat-shock protein but significantly increased after stimulation with staphylococcal enterotoxins C1 and D. Those cells showed increased cytolytic activity against target cells cross-linked by anti-Vβ5.2/5.3 and produced a large amount of interferon-γ These results indicated that CD4+Vβ5.2/5.3+ iIELs were preferentially activated in the inflamed lesions of Crohn's disease and may play a possible role in the triggering and progression of human inflammatory intestinal disease.
UR - http://www.scopus.com/inward/record.url?scp=0029965229&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029965229&partnerID=8YFLogxK
U2 - 10.1006/clin.1996.0022
DO - 10.1006/clin.1996.0022
M3 - Article
C2 - 8625555
AN - SCOPUS:0029965229
VL - 78
SP - 130
EP - 139
JO - Clinical Immunology
JF - Clinical Immunology
SN - 1521-6616
IS - 2
ER -