Abstract
To analyze the nature of intestinal intraepithelial lymphocytes (iIELs) in inflammatory intestinal disease, we established T cell lines of iIELs isolated from endoscopic biopsied ileal and colonic mucosa of Crohn's disease patients. Seven T cell lines from the inflamed terminal ileum of 13 patients, but none of 16 T cell lines from normal terminal ileum, have shown a deviation of T cell receptor variable region gene usage and were enriched in the proportion of CD4+Vβ5.2/5.3+ cells. CD4+Vβ5.2/5.3+ cells in the T cell lines were not increased after stimulation with purified protein derivatives or 65-kDa heat-shock protein but significantly increased after stimulation with staphylococcal enterotoxins C1 and D. Those cells showed increased cytolytic activity against target cells cross-linked by anti-Vβ5.2/5.3 and produced a large amount of interferon-γ These results indicated that CD4+Vβ5.2/5.3+ iIELs were preferentially activated in the inflamed lesions of Crohn's disease and may play a possible role in the triggering and progression of human inflammatory intestinal disease.
Original language | English |
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Pages (from-to) | 130-139 |
Number of pages | 10 |
Journal | Clinical Immunology and Immunopathology |
Volume | 78 |
Issue number | 2 |
DOIs | |
Publication status | Published - 1996 |
Externally published | Yes |
ASJC Scopus subject areas
- Immunology and Allergy
- Pathology and Forensic Medicine
- Immunology