TY - JOUR
T1 - Preferential Alternative Splicing in Cancer Generates a K-sam Messenger RNA with Higher Transforming Activity
AU - Itoh, Hiroshi
AU - Hattori, Yutaka
AU - Sakamoto, Hiromi
AU - Ishii, Hideshi
AU - Kishi, Tatsuya
AU - Sasaki, Hiroki
AU - Yoshida, Teruhiko
AU - Koono, Masashi
AU - Sugimura, Takashi
AU - Terada, Masaaki
PY - 1994/6/15
Y1 - 1994/6/15
N2 - K-sam, also designated fibroblast growth factor receptor 2/BEK, was originally cloned from a stomach cancer cell line KATO-III. The gene is amplified and overexpressed preferentially in poorly differentiated types of stomach cancers. The major K-sam transcript in KATO-III cells encodes a receptor protein with a truncated carboxyl terminus and with a high-affinity binding site for keratinocyte growth factor. This truncated type is produced by an alternative splicing mechanism, and in normal tissues, the truncated type is far less prevalent than the untruncated form. The variant K-sam complementary DNA lacks tyrosine 769, which is a putative phospholipase Cγ1 association site, and showed a higher transforming activity to NIH3T3 cells than the untruncated form, which is identical with the keratinocyte growth factor receptor.
AB - K-sam, also designated fibroblast growth factor receptor 2/BEK, was originally cloned from a stomach cancer cell line KATO-III. The gene is amplified and overexpressed preferentially in poorly differentiated types of stomach cancers. The major K-sam transcript in KATO-III cells encodes a receptor protein with a truncated carboxyl terminus and with a high-affinity binding site for keratinocyte growth factor. This truncated type is produced by an alternative splicing mechanism, and in normal tissues, the truncated type is far less prevalent than the untruncated form. The variant K-sam complementary DNA lacks tyrosine 769, which is a putative phospholipase Cγ1 association site, and showed a higher transforming activity to NIH3T3 cells than the untruncated form, which is identical with the keratinocyte growth factor receptor.
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M3 - Article
C2 - 8205545
AN - SCOPUS:0028305121
VL - 54
SP - 3237
EP - 3241
JO - Journal of Cancer Research
JF - Journal of Cancer Research
SN - 0008-5472
IS - 12
ER -