Preferential induction of Th17 cells in vitro and in vivo by Fucogalactan from Ganoderma lucidum (Reishi)

Hideyuki Yoshida, Mayu Suzuki, Ryota Sakaguchi, Ito Tani, Hitoshi Kotani, Norimasa Shudo, Akihiko Yoshimura

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The mushroom known as Reishi (Ganoderma lucidum) has been used as an herbal medicine for tumor treatment and immune system activation. Because its effects on the differentiation of effector T helper cells have not yet been fully understood, we investigated the effects of Reishi and those of its principal ingredient, β-glucan, on the activation of dendritic cells and the differentiation of Th17 cells. Reishi extracts as well as purified β-glucan (Curdran) activated DCs and caused them to produce large amounts of IL-23. β-glucan also enhanced and sustained the transcription of IL-23p19. The MEK-ERK signaling pathway positively regulates IL-23p19 transcription in β-glucan-stimulated DCs. In a mixed leukocyte reaction, Reishi-stimulated DCs preferentially induced Th17 cells. Furthermore, orally-administrated Reishi increased the percentages of Th17 cells and the transcription levels of antimicrobial peptides. Our results show that Reishi and β-glucan activate DCs to produce large amounts of IL-23, which induces Th17 differentiation both in vitro and in vivo.

Original languageEnglish
Pages (from-to)174-180
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume422
Issue number1
DOIs
Publication statusPublished - 2012 May 25

Fingerprint

Reishi
Th17 Cells
Glucans
Interleukin-23 Subunit p19
Transcription
Interleukin-23
Chemical activation
Immune system
Mitogen-Activated Protein Kinase Kinases
Mixed Lymphocyte Culture Test
Tumors
MAP Kinase Signaling System
Herbal Medicine
Agaricales
Helper-Inducer T-Lymphocytes
In Vitro Techniques
fucogalactan
Dendritic Cells
Peptides
Cell Differentiation

Keywords

  • β-Glucan
  • Dendritic cell
  • ERK
  • Helper T cell
  • Interleukin

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Preferential induction of Th17 cells in vitro and in vivo by Fucogalactan from Ganoderma lucidum (Reishi). / Yoshida, Hideyuki; Suzuki, Mayu; Sakaguchi, Ryota; Tani, Ito; Kotani, Hitoshi; Shudo, Norimasa; Yoshimura, Akihiko.

In: Biochemical and Biophysical Research Communications, Vol. 422, No. 1, 25.05.2012, p. 174-180.

Research output: Contribution to journalArticle

Yoshida, Hideyuki ; Suzuki, Mayu ; Sakaguchi, Ryota ; Tani, Ito ; Kotani, Hitoshi ; Shudo, Norimasa ; Yoshimura, Akihiko. / Preferential induction of Th17 cells in vitro and in vivo by Fucogalactan from Ganoderma lucidum (Reishi). In: Biochemical and Biophysical Research Communications. 2012 ; Vol. 422, No. 1. pp. 174-180.
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