Preferential localization of SSEA-4 in interfaces between blastomeres of mouse preimplantaion embryos

Ban Sato, Yohko U. Katagiri, Kenji Miyado, Hidenori Akutsu, Yoshitaka Miyagawa, Yasuomi Horiuchi, Hideki Nakajima, Hajime Okita, Akihiro Umezawa, Jun ichi Hata, Junichiro Fujimoto, Kiyotaka Toshimori, Nobutaka Kiyokawa

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The monoclonal antibody 6E2 raised against the embryonal carcinoma cell line NCR-G3 had been shown to also react with human germ cells. Thin-layer chromatography (TLC) immunostaining revealed that 6E2 specifically reacts with sialosylglobopentaosylceramide (sialylGb5), which carries an epitope of stage-specific embryonic antigen-4 (SSEA-4), known as an important cell surface marker of embryogenesis. The immunostaining of mouse preimplantation embryos without fixation showed that the binding of 6E2 caused the clustering and consequent accumulation of sialylGb5 at the interface between blastomeres. These results suggest that SSEA-4 actively moves on the cell surface and readily accumulates between blastomeres after binding of 6E2.

Original languageEnglish
Pages (from-to)838-843
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume364
Issue number4
DOIs
Publication statusPublished - 2007 Dec 28

Keywords

  • Embryonal carcinoma cells
  • Embryonic stem cells
  • Immunostaining
  • Preimplantation embryo
  • SSEA-4
  • SialylGb5

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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