Preferential transcription of HTLV-I LTR in cell-free extracts of human T cells producing HTLV-I viral proteins

Norisada Matsunami, Haruhiko Siomi, Masakazu Hatanaka, Yoshio Yaoita, Tasuku Honjo

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Abstract

The promoters of the adenovlrus 2 major late gene, the mouse β-globin gene, the mouse lmmunoglobulin VH gene and the LTR of the human T-lympho-tropic retrovirus type I were tested for their transcription activities in cell-free extracts of four cell lines; HeLa, CESS (Epstein-Barr virus-transformed human B cell line), MT-1 (HTLV-I-infected human T cell line without viral protein synthesis), and MT-2 (HTLV-I-infected hitman T cell line producing viral proteins). LTR was preferentially transcribed in the extracts of MT-2 although the other three genes were transcribed with relatively constant efficiencies in different extracts. The results agree well with the previous in vivo studies on the promoter activity of HTLV-I LTR. Mixing of HeLa and MT-2 extracts revealed the presence of a LTR-specific stimulating activity in MT-2 extracts.

Original languageEnglish
Pages (from-to)4779-4786
Number of pages8
JournalNucleic acids research
Volume14
Issue number12
DOIs
Publication statusPublished - 1986 Jun 25
Externally publishedYes

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ASJC Scopus subject areas

  • Genetics

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