Abstract
The promoters of the adenovlrus 2 major late gene, the mouse β-globin gene, the mouse lmmunoglobulin VH gene and the LTR of the human T-lympho-tropic retrovirus type I were tested for their transcription activities in cell-free extracts of four cell lines; HeLa, CESS (Epstein-Barr virus-transformed human B cell line), MT-1 (HTLV-I-infected human T cell line without viral protein synthesis), and MT-2 (HTLV-I-infected hitman T cell line producing viral proteins). LTR was preferentially transcribed in the extracts of MT-2 although the other three genes were transcribed with relatively constant efficiencies in different extracts. The results agree well with the previous in vivo studies on the promoter activity of HTLV-I LTR. Mixing of HeLa and MT-2 extracts revealed the presence of a LTR-specific stimulating activity in MT-2 extracts.
| Original language | English |
|---|---|
| Pages (from-to) | 4779-4786 |
| Number of pages | 8 |
| Journal | Nucleic Acids Research |
| Volume | 14 |
| Issue number | 12 |
| DOIs | |
| Publication status | Published - 1986 Jun 25 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Statistics, Probability and Uncertainty
- Applied Mathematics
- Health, Toxicology and Mutagenesis
- Toxicology
- Genetics(clinical)
- Genetics
Cite this
Preferential transcription of HTLV-I LTR in cell-free extracts of human T cells producing HTLV-I viral proteins. / Matsunami, Norisada; Siomi, Haruhiko; Hatanaka, Masakazu; Yaoita, Yoshio; Honjo, Tasuku.
In: Nucleic Acids Research, Vol. 14, No. 12, 25.06.1986, p. 4779-4786.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Preferential transcription of HTLV-I LTR in cell-free extracts of human T cells producing HTLV-I viral proteins
AU - Matsunami, Norisada
AU - Siomi, Haruhiko
AU - Hatanaka, Masakazu
AU - Yaoita, Yoshio
AU - Honjo, Tasuku
PY - 1986/6/25
Y1 - 1986/6/25
N2 - The promoters of the adenovlrus 2 major late gene, the mouse β-globin gene, the mouse lmmunoglobulin VH gene and the LTR of the human T-lympho-tropic retrovirus type I were tested for their transcription activities in cell-free extracts of four cell lines; HeLa, CESS (Epstein-Barr virus-transformed human B cell line), MT-1 (HTLV-I-infected human T cell line without viral protein synthesis), and MT-2 (HTLV-I-infected hitman T cell line producing viral proteins). LTR was preferentially transcribed in the extracts of MT-2 although the other three genes were transcribed with relatively constant efficiencies in different extracts. The results agree well with the previous in vivo studies on the promoter activity of HTLV-I LTR. Mixing of HeLa and MT-2 extracts revealed the presence of a LTR-specific stimulating activity in MT-2 extracts.
AB - The promoters of the adenovlrus 2 major late gene, the mouse β-globin gene, the mouse lmmunoglobulin VH gene and the LTR of the human T-lympho-tropic retrovirus type I were tested for their transcription activities in cell-free extracts of four cell lines; HeLa, CESS (Epstein-Barr virus-transformed human B cell line), MT-1 (HTLV-I-infected human T cell line without viral protein synthesis), and MT-2 (HTLV-I-infected hitman T cell line producing viral proteins). LTR was preferentially transcribed in the extracts of MT-2 although the other three genes were transcribed with relatively constant efficiencies in different extracts. The results agree well with the previous in vivo studies on the promoter activity of HTLV-I LTR. Mixing of HeLa and MT-2 extracts revealed the presence of a LTR-specific stimulating activity in MT-2 extracts.
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U2 - 10.1093/nar/14.12.4779
DO - 10.1093/nar/14.12.4779
M3 - Article
VL - 14
SP - 4779
EP - 4786
JO - Nucleic Acids Research
JF - Nucleic Acids Research
SN - 0305-1048
IS - 12
ER -