Preferential transcription of HTLV-I LTR in cell-free extracts of human T cells producing HTLV-I viral proteins

Norisada Matsunami, Haruhiko Siomi, Masakazu Hatanaka, Yoshio Yaoita, Tasuku Honjo

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The promoters of the adenovlrus 2 major late gene, the mouse β-globin gene, the mouse lmmunoglobulin VH gene and the LTR of the human T-lympho-tropic retrovirus type I were tested for their transcription activities in cell-free extracts of four cell lines; HeLa, CESS (Epstein-Barr virus-transformed human B cell line), MT-1 (HTLV-I-infected human T cell line without viral protein synthesis), and MT-2 (HTLV-I-infected hitman T cell line producing viral proteins). LTR was preferentially transcribed in the extracts of MT-2 although the other three genes were transcribed with relatively constant efficiencies in different extracts. The results agree well with the previous in vivo studies on the promoter activity of HTLV-I LTR. Mixing of HeLa and MT-2 extracts revealed the presence of a LTR-specific stimulating activity in MT-2 extracts.

Original languageEnglish
Pages (from-to)4779-4786
Number of pages8
JournalNucleic Acids Research
Volume14
Issue number12
DOIs
Publication statusPublished - 1986 Jun 25
Externally publishedYes

Fingerprint

Human T-lymphotropic virus 1
T-cells
Viral Proteins
Transcription
Cell Extracts
Genes
Gene
Proteins
Protein
T-Lymphocytes
Cell Line
Line
Cell
Promoter
Mouse
Cells
Tropics
Globins
B Cells
Protein Synthesis

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

Cite this

Preferential transcription of HTLV-I LTR in cell-free extracts of human T cells producing HTLV-I viral proteins. / Matsunami, Norisada; Siomi, Haruhiko; Hatanaka, Masakazu; Yaoita, Yoshio; Honjo, Tasuku.

In: Nucleic Acids Research, Vol. 14, No. 12, 25.06.1986, p. 4779-4786.

Research output: Contribution to journalArticle

Matsunami, Norisada ; Siomi, Haruhiko ; Hatanaka, Masakazu ; Yaoita, Yoshio ; Honjo, Tasuku. / Preferential transcription of HTLV-I LTR in cell-free extracts of human T cells producing HTLV-I viral proteins. In: Nucleic Acids Research. 1986 ; Vol. 14, No. 12. pp. 4779-4786.
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