TY - JOUR
T1 - Preferential transcription of HTLV-I LTR in cell-free extracts of human T cells producing HTLV-I viral proteins
AU - Matsunami, Norisada
AU - Siomi, Haruhiko
AU - Hatanaka, Masakazu
AU - Yaoita, Yoshio
AU - Honjo, Tasuku
N1 - Funding Information:
ACKNOWLEDGEMENT We thank Ms. Y. Utsunomiya for excellent technical assistance. Ms. K. Hirano for preparation of the manuscript. This investigation was supported in part by grants from the Ministry of Education, Science and Culture of Japan.
PY - 1986/6/25
Y1 - 1986/6/25
N2 - The promoters of the adenovlrus 2 major late gene, the mouse β-globin gene, the mouse lmmunoglobulin VH gene and the LTR of the human T-lympho-tropic retrovirus type I were tested for their transcription activities in cell-free extracts of four cell lines; HeLa, CESS (Epstein-Barr virus-transformed human B cell line), MT-1 (HTLV-I-infected human T cell line without viral protein synthesis), and MT-2 (HTLV-I-infected hitman T cell line producing viral proteins). LTR was preferentially transcribed in the extracts of MT-2 although the other three genes were transcribed with relatively constant efficiencies in different extracts. The results agree well with the previous in vivo studies on the promoter activity of HTLV-I LTR. Mixing of HeLa and MT-2 extracts revealed the presence of a LTR-specific stimulating activity in MT-2 extracts.
AB - The promoters of the adenovlrus 2 major late gene, the mouse β-globin gene, the mouse lmmunoglobulin VH gene and the LTR of the human T-lympho-tropic retrovirus type I were tested for their transcription activities in cell-free extracts of four cell lines; HeLa, CESS (Epstein-Barr virus-transformed human B cell line), MT-1 (HTLV-I-infected human T cell line without viral protein synthesis), and MT-2 (HTLV-I-infected hitman T cell line producing viral proteins). LTR was preferentially transcribed in the extracts of MT-2 although the other three genes were transcribed with relatively constant efficiencies in different extracts. The results agree well with the previous in vivo studies on the promoter activity of HTLV-I LTR. Mixing of HeLa and MT-2 extracts revealed the presence of a LTR-specific stimulating activity in MT-2 extracts.
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U2 - 10.1093/nar/14.12.4779
DO - 10.1093/nar/14.12.4779
M3 - Article
C2 - 3014437
AN - SCOPUS:0022579979
VL - 14
SP - 4779
EP - 4786
JO - Nucleic Acids Research
JF - Nucleic Acids Research
SN - 0305-1048
IS - 12
ER -