Preinspiratory calcium rise in putative pre-Bötzinger complex astrocytes

Yasumasa Okada, Takuya Sasaki, Yoshitaka Oku, Naoya Takahashi, Megumi Seki, Sakiko Ujita, Kenji Tanaka, Norio Matsuki, Yuji Ikegaya

Research output: Contribution to journalArticle

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Abstract

The neural inspiratory activity originates from a ventrolateral medullary region called the pre-Bötzinger complex (preBötC), yet the mechanism underlying respiratory rhythmogenesis is not completely understood. Recently, the role of not only neurons but astrocytes in the central respiratory control has attracted considerable attention. Here we report our discovery that an intracellular calcium rise in a subset of putative astrocytes precedes inspiratory neuronal firing in rhythmically active slices. Functional calcium imaging from hundreds of preBötC cells revealed that a subset of putative astrocytes exhibited rhythmic calcium elevations preceding inspiratory neuronal activity with a time lag of approximately 2 s. These preinspiratory putative astrocytes maintained their rhythmic activities even during the blockade of neuronal activity with tetrodotoxin, whereas the rhythm frequency was lowered and the intercellular phases of these rhythms were decoupled. In addition, optogenetic stimulation of preBötC putative astrocytes induced firing of inspiratory neurons. These findings raise the possibility that astrocytes in the preBötC are actively involved in respiratory rhythm generation in rhythmically active slices.

Original languageEnglish
Pages (from-to)4933-4944
Number of pages12
JournalJournal of Physiology
Volume590
Issue number19
DOIs
Publication statusPublished - 2012 Oct

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Astrocytes
Calcium
Optogenetics
Neurons
Tetrodotoxin

ASJC Scopus subject areas

  • Physiology

Cite this

Okada, Y., Sasaki, T., Oku, Y., Takahashi, N., Seki, M., Ujita, S., ... Ikegaya, Y. (2012). Preinspiratory calcium rise in putative pre-Bötzinger complex astrocytes. Journal of Physiology, 590(19), 4933-4944. https://doi.org/10.1113/jphysiol.2012.231464

Preinspiratory calcium rise in putative pre-Bötzinger complex astrocytes. / Okada, Yasumasa; Sasaki, Takuya; Oku, Yoshitaka; Takahashi, Naoya; Seki, Megumi; Ujita, Sakiko; Tanaka, Kenji; Matsuki, Norio; Ikegaya, Yuji.

In: Journal of Physiology, Vol. 590, No. 19, 10.2012, p. 4933-4944.

Research output: Contribution to journalArticle

Okada, Y, Sasaki, T, Oku, Y, Takahashi, N, Seki, M, Ujita, S, Tanaka, K, Matsuki, N & Ikegaya, Y 2012, 'Preinspiratory calcium rise in putative pre-Bötzinger complex astrocytes', Journal of Physiology, vol. 590, no. 19, pp. 4933-4944. https://doi.org/10.1113/jphysiol.2012.231464
Okada Y, Sasaki T, Oku Y, Takahashi N, Seki M, Ujita S et al. Preinspiratory calcium rise in putative pre-Bötzinger complex astrocytes. Journal of Physiology. 2012 Oct;590(19):4933-4944. https://doi.org/10.1113/jphysiol.2012.231464
Okada, Yasumasa ; Sasaki, Takuya ; Oku, Yoshitaka ; Takahashi, Naoya ; Seki, Megumi ; Ujita, Sakiko ; Tanaka, Kenji ; Matsuki, Norio ; Ikegaya, Yuji. / Preinspiratory calcium rise in putative pre-Bötzinger complex astrocytes. In: Journal of Physiology. 2012 ; Vol. 590, No. 19. pp. 4933-4944.
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