Preliminary human application of optical coherence tomography for quantification and localization of primordial follicles aimed at effective ovarian tissue transplantation

Seido Takae, Kosuke Tsukada, Ichiro Maeda, Naoki Okamoto, Yorino Sato, Haruhiro Kondo, Kiemi Shinya, Yuki Motani, Nao Suzuki

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Purpose: The purpose of this study was to evaluate the possible clinical application of optical coherence tomography for assessing ovarian reserve in individual specimens of human ovarian tissue for fertility preservation. Methods: Ovarian tissue examination by optical coherence tomography was performed before ovarian tissue cryopreservation. Three of the four subjects had hematological disease or cancer, and they faced a threat to their fertility due to impending chemotherapy. One patient underwent ovarian tissue extraction for in vitro activation of dormant follicles as fertility treatment. Results: The current full-field optical coherence tomography technique can detect primordial follicles in non-fixed and non-embedded human ovarian tissue. These images are well correlated with histological evaluation and the ovarian reserve test, including follicle counts. Conclusion: It was demonstrated that optical coherence tomography could assess localization of primordial follicles and ovarian reserve in specimens of non-fixed human ovarian cortex, although optimization for examination of human ovarian tissue is needed for clinical application. Additionally, this technique holds the possibility of assessing the ovarian reserve of patients with unevaluable ovarian reserve. Trial registration number: UMIN000023141

Original languageEnglish
Pages (from-to)1-10
Number of pages10
JournalJournal of Assisted Reproduction and Genetics
DOIs
Publication statusAccepted/In press - 2018 Apr 2

Fingerprint

Tissue Transplantation
Optical Coherence Tomography
Fertility
Fertility Preservation
Tissue Preservation
Hematologic Diseases
Cryopreservation
Ovarian Reserve
Drug Therapy
Neoplasms

Keywords

  • Fertility preservation
  • Optical coherence tomography
  • Ovarian reserve
  • Ovarian tissue cryopreservation
  • Ovarian tissue transplantation

ASJC Scopus subject areas

  • Reproductive Medicine
  • Genetics
  • Obstetrics and Gynaecology
  • Developmental Biology
  • Genetics(clinical)

Cite this

Preliminary human application of optical coherence tomography for quantification and localization of primordial follicles aimed at effective ovarian tissue transplantation. / Takae, Seido; Tsukada, Kosuke; Maeda, Ichiro; Okamoto, Naoki; Sato, Yorino; Kondo, Haruhiro; Shinya, Kiemi; Motani, Yuki; Suzuki, Nao.

In: Journal of Assisted Reproduction and Genetics, 02.04.2018, p. 1-10.

Research output: Contribution to journalArticle

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AU - Takae, Seido

AU - Tsukada, Kosuke

AU - Maeda, Ichiro

AU - Okamoto, Naoki

AU - Sato, Yorino

AU - Kondo, Haruhiro

AU - Shinya, Kiemi

AU - Motani, Yuki

AU - Suzuki, Nao

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