Premeiotic germ cell defect in seminiferous tubules of Atm-null testis

Keiyo Takubo, Atsushi Hirao, Masako Ohmura, Masaki Azuma, Fumio Arai, Go Nagamatsu, Toshio Suda

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Lifelong spermatogenesis is maintained by coordinated sequential processes including self-renewal of stem cells, proliferation of spermatogonial cells, meiotic division, and spermiogenesis. It has been shown that ataxia telangiectasia-mutated (ATM) is required for meiotic division of the seminiferous tubules. Here, we show that, in addition to its role in meiosis, ATM has a pivotal role in premeiotic germ cell maintenance. ATM is activated in premeiotic spermatogonial cells and the Atm-null testis shows progressive degeneration. In Atm-null testicular cells, differing from bone marrow cells of Atm-null mice, reactive oxygen species-mediated p16Ink4a activation does not occur in Atm-null premeiotic germ cells, which suggests the involvement of different signaling pathways from bone marrow defects. Although Atm-null bone marrow undergoes p16Ink4a-mediated cellular senescence program, Atm-null premeiotic germ cells exhibited cell cycle arrest and apoptotic elimination of premeiotic germ cells, which is different from p16Ink4a-mediated senescence.

Original languageEnglish
Pages (from-to)993-998
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume351
Issue number4
DOIs
Publication statusPublished - 2006 Dec 29

Fingerprint

Seminiferous Tubules
Germ Cells
Ataxia Telangiectasia
Testis
Cells
Null Lymphocytes
Defects
Spermatogenesis
Bone
Bone Marrow
Cell Aging
Meiosis
Cell Cycle Checkpoints
Bone Marrow Cells
Cell Division
Reactive Oxygen Species
Maintenance
Cell Proliferation
Cell proliferation
Stem cells

Keywords

  • Apoptosis
  • ATM
  • Cell cycle arrest
  • Premeiotic germ cells

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Takubo, K., Hirao, A., Ohmura, M., Azuma, M., Arai, F., Nagamatsu, G., & Suda, T. (2006). Premeiotic germ cell defect in seminiferous tubules of Atm-null testis. Biochemical and Biophysical Research Communications, 351(4), 993-998. https://doi.org/10.1016/j.bbrc.2006.10.145

Premeiotic germ cell defect in seminiferous tubules of Atm-null testis. / Takubo, Keiyo; Hirao, Atsushi; Ohmura, Masako; Azuma, Masaki; Arai, Fumio; Nagamatsu, Go; Suda, Toshio.

In: Biochemical and Biophysical Research Communications, Vol. 351, No. 4, 29.12.2006, p. 993-998.

Research output: Contribution to journalArticle

Takubo, K, Hirao, A, Ohmura, M, Azuma, M, Arai, F, Nagamatsu, G & Suda, T 2006, 'Premeiotic germ cell defect in seminiferous tubules of Atm-null testis', Biochemical and Biophysical Research Communications, vol. 351, no. 4, pp. 993-998. https://doi.org/10.1016/j.bbrc.2006.10.145
Takubo, Keiyo ; Hirao, Atsushi ; Ohmura, Masako ; Azuma, Masaki ; Arai, Fumio ; Nagamatsu, Go ; Suda, Toshio. / Premeiotic germ cell defect in seminiferous tubules of Atm-null testis. In: Biochemical and Biophysical Research Communications. 2006 ; Vol. 351, No. 4. pp. 993-998.
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