Abstract
Lifelong spermatogenesis is maintained by coordinated sequential processes including self-renewal of stem cells, proliferation of spermatogonial cells, meiotic division, and spermiogenesis. It has been shown that ataxia telangiectasia-mutated (ATM) is required for meiotic division of the seminiferous tubules. Here, we show that, in addition to its role in meiosis, ATM has a pivotal role in premeiotic germ cell maintenance. ATM is activated in premeiotic spermatogonial cells and the Atm-null testis shows progressive degeneration. In Atm-null testicular cells, differing from bone marrow cells of Atm-null mice, reactive oxygen species-mediated p16Ink4a activation does not occur in Atm-null premeiotic germ cells, which suggests the involvement of different signaling pathways from bone marrow defects. Although Atm-null bone marrow undergoes p16Ink4a-mediated cellular senescence program, Atm-null premeiotic germ cells exhibited cell cycle arrest and apoptotic elimination of premeiotic germ cells, which is different from p16Ink4a-mediated senescence.
| Original language | English |
|---|---|
| Pages (from-to) | 993-998 |
| Number of pages | 6 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 351 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2006 Dec 29 |
Fingerprint
Keywords
- Apoptosis
- ATM
- Cell cycle arrest
- Premeiotic germ cells
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Premeiotic germ cell defect in seminiferous tubules of Atm-null testis. / Takubo, Keiyo; Hirao, Atsushi; Ohmura, Masako; Azuma, Masaki; Arai, Fumio; Nagamatsu, Go; Suda, Toshio.
In: Biochemical and Biophysical Research Communications, Vol. 351, No. 4, 29.12.2006, p. 993-998.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Premeiotic germ cell defect in seminiferous tubules of Atm-null testis
AU - Takubo, Keiyo
AU - Hirao, Atsushi
AU - Ohmura, Masako
AU - Azuma, Masaki
AU - Arai, Fumio
AU - Nagamatsu, Go
AU - Suda, Toshio
PY - 2006/12/29
Y1 - 2006/12/29
N2 - Lifelong spermatogenesis is maintained by coordinated sequential processes including self-renewal of stem cells, proliferation of spermatogonial cells, meiotic division, and spermiogenesis. It has been shown that ataxia telangiectasia-mutated (ATM) is required for meiotic division of the seminiferous tubules. Here, we show that, in addition to its role in meiosis, ATM has a pivotal role in premeiotic germ cell maintenance. ATM is activated in premeiotic spermatogonial cells and the Atm-null testis shows progressive degeneration. In Atm-null testicular cells, differing from bone marrow cells of Atm-null mice, reactive oxygen species-mediated p16Ink4a activation does not occur in Atm-null premeiotic germ cells, which suggests the involvement of different signaling pathways from bone marrow defects. Although Atm-null bone marrow undergoes p16Ink4a-mediated cellular senescence program, Atm-null premeiotic germ cells exhibited cell cycle arrest and apoptotic elimination of premeiotic germ cells, which is different from p16Ink4a-mediated senescence.
AB - Lifelong spermatogenesis is maintained by coordinated sequential processes including self-renewal of stem cells, proliferation of spermatogonial cells, meiotic division, and spermiogenesis. It has been shown that ataxia telangiectasia-mutated (ATM) is required for meiotic division of the seminiferous tubules. Here, we show that, in addition to its role in meiosis, ATM has a pivotal role in premeiotic germ cell maintenance. ATM is activated in premeiotic spermatogonial cells and the Atm-null testis shows progressive degeneration. In Atm-null testicular cells, differing from bone marrow cells of Atm-null mice, reactive oxygen species-mediated p16Ink4a activation does not occur in Atm-null premeiotic germ cells, which suggests the involvement of different signaling pathways from bone marrow defects. Although Atm-null bone marrow undergoes p16Ink4a-mediated cellular senescence program, Atm-null premeiotic germ cells exhibited cell cycle arrest and apoptotic elimination of premeiotic germ cells, which is different from p16Ink4a-mediated senescence.
KW - Apoptosis
KW - ATM
KW - Cell cycle arrest
KW - Premeiotic germ cells
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UR - http://www.scopus.com/inward/citedby.url?scp=33750806537&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2006.10.145
DO - 10.1016/j.bbrc.2006.10.145
M3 - Article
C2 - 17097049
AN - SCOPUS:33750806537
VL - 351
SP - 993
EP - 998
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 4
ER -