Preoperative aqueous cytokine levels are associated with endothelial cell loss after descemet’s stripping automated endothelial keratoplasty

Hiroyuki Yazu, Takefumi Yamaguchi, Naohiko Aketa, Kazunari Higa, Terumasa Suzuki, Yukari Yagi-Yaguchi, Yoshiyuki Satake, Takayuki Abe, Kazuo Tsubota, Jun Shimazaki

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Purpose: To evaluate the association between endothelial cell density (ECD) after Descemet’s stripping automated endothelial keratoplasty (DSAEK) and preoperative cytokine levels in the aqueous humor (AqH). Methods: This prospective consecutive case series included 97 consecutive patients who underwent DSAEK (64 eyes) or cataract surgery (33 eyes). AqH samples were collected at the beginning of each surgery. The levels of cytokines (IL-1α, IL-1β, IL-4, IL-6, IL-8, IL-10, IL-12p70, IL-13, IL-17A, IFN-α, IFN-γ, monocyte chemotactic protein [MCP]-1, E-selectin, P-selectin, and soluble intercellular adhesion molecule [sICAM]-1) in the AqH were measured by multiplex beads immunoassay. The correlations between preoperative aqueous cytokine levels and the ECD at 12 months after DSAEK were analyzed. Results: The ECD decreased from 2747 ± 259 cells/mm2in the donor graft to 1235 ± 607 cells/mm2at 12 months after DSAEK. In all subjects undergoing DSAEK, the postoperative ECD at 12 months was significantly correlated with the preoperative levels of MCP-1 (r = −0.467, 95% confidence interval [CI]: −0.650 to −0.222, P = 0.0003). In an analysis excluding Fuchs endothelial corneal dystrophy (11 eyes), the ECD at 12 months after DSAEK was significantly correlated with preoperative levels of IL-17A (r = −0.635, 95% CI: −0.819 to −0.319, P = 0.0004), MCP-1 (r = −0.605, 95% CI: −0.779 to −0.345, P < 0.0001), IFN-γ (r = −0.633, 95% CI: −0.796 to −0.385, P < 0.0001), E-selectin (r = −0.516, 95% CI: −0.756 to −0.276, P = 0.0004), and sICAM-1 (r = −0.537, 95% CI: −0.735 to −0.253, P = 0.0005). Conclusions: Higher preoperative levels of IL-17A, MCP-1, IFN-γ, E-selectin, and sICAM-1 in the AqH were associated with lower ECD after DSAEK for bullous keratopathy.

Original languageEnglish
Pages (from-to)612-620
Number of pages9
JournalInvestigative Ophthalmology and Visual Science
Volume59
Issue number2
DOIs
Publication statusPublished - 2018 Feb 1

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Descemet Stripping Endothelial Keratoplasty
Endothelial Cells
Cytokines
Aqueous Humor
Cell Count
Chemokine CCL2
Confidence Intervals
E-Selectin
Interleukin-17
Intercellular Adhesion Molecule-1
Interleukin-1
Fuchs' Endothelial Dystrophy
P-Selectin
Interleukin-13
Interleukin-8
Immunoassay
Interleukin-4
Interleukin-10
Cataract
Interleukin-6

Keywords

  • Aqueous humor
  • Cytokine
  • Descemet’s stripping automated endothelial keratoplasty
  • Endothelial cell density

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Preoperative aqueous cytokine levels are associated with endothelial cell loss after descemet’s stripping automated endothelial keratoplasty. / Yazu, Hiroyuki; Yamaguchi, Takefumi; Aketa, Naohiko; Higa, Kazunari; Suzuki, Terumasa; Yagi-Yaguchi, Yukari; Satake, Yoshiyuki; Abe, Takayuki; Tsubota, Kazuo; Shimazaki, Jun.

In: Investigative Ophthalmology and Visual Science, Vol. 59, No. 2, 01.02.2018, p. 612-620.

Research output: Contribution to journalArticle

Yazu, Hiroyuki ; Yamaguchi, Takefumi ; Aketa, Naohiko ; Higa, Kazunari ; Suzuki, Terumasa ; Yagi-Yaguchi, Yukari ; Satake, Yoshiyuki ; Abe, Takayuki ; Tsubota, Kazuo ; Shimazaki, Jun. / Preoperative aqueous cytokine levels are associated with endothelial cell loss after descemet’s stripping automated endothelial keratoplasty. In: Investigative Ophthalmology and Visual Science. 2018 ; Vol. 59, No. 2. pp. 612-620.
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title = "Preoperative aqueous cytokine levels are associated with endothelial cell loss after descemet’s stripping automated endothelial keratoplasty",
abstract = "Purpose: To evaluate the association between endothelial cell density (ECD) after Descemet’s stripping automated endothelial keratoplasty (DSAEK) and preoperative cytokine levels in the aqueous humor (AqH). Methods: This prospective consecutive case series included 97 consecutive patients who underwent DSAEK (64 eyes) or cataract surgery (33 eyes). AqH samples were collected at the beginning of each surgery. The levels of cytokines (IL-1α, IL-1β, IL-4, IL-6, IL-8, IL-10, IL-12p70, IL-13, IL-17A, IFN-α, IFN-γ, monocyte chemotactic protein [MCP]-1, E-selectin, P-selectin, and soluble intercellular adhesion molecule [sICAM]-1) in the AqH were measured by multiplex beads immunoassay. The correlations between preoperative aqueous cytokine levels and the ECD at 12 months after DSAEK were analyzed. Results: The ECD decreased from 2747 ± 259 cells/mm2in the donor graft to 1235 ± 607 cells/mm2at 12 months after DSAEK. In all subjects undergoing DSAEK, the postoperative ECD at 12 months was significantly correlated with the preoperative levels of MCP-1 (r = −0.467, 95{\%} confidence interval [CI]: −0.650 to −0.222, P = 0.0003). In an analysis excluding Fuchs endothelial corneal dystrophy (11 eyes), the ECD at 12 months after DSAEK was significantly correlated with preoperative levels of IL-17A (r = −0.635, 95{\%} CI: −0.819 to −0.319, P = 0.0004), MCP-1 (r = −0.605, 95{\%} CI: −0.779 to −0.345, P < 0.0001), IFN-γ (r = −0.633, 95{\%} CI: −0.796 to −0.385, P < 0.0001), E-selectin (r = −0.516, 95{\%} CI: −0.756 to −0.276, P = 0.0004), and sICAM-1 (r = −0.537, 95{\%} CI: −0.735 to −0.253, P = 0.0005). Conclusions: Higher preoperative levels of IL-17A, MCP-1, IFN-γ, E-selectin, and sICAM-1 in the AqH were associated with lower ECD after DSAEK for bullous keratopathy.",
keywords = "Aqueous humor, Cytokine, Descemet’s stripping automated endothelial keratoplasty, Endothelial cell density",
author = "Hiroyuki Yazu and Takefumi Yamaguchi and Naohiko Aketa and Kazunari Higa and Terumasa Suzuki and Yukari Yagi-Yaguchi and Yoshiyuki Satake and Takayuki Abe and Kazuo Tsubota and Jun Shimazaki",
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T1 - Preoperative aqueous cytokine levels are associated with endothelial cell loss after descemet’s stripping automated endothelial keratoplasty

AU - Yazu, Hiroyuki

AU - Yamaguchi, Takefumi

AU - Aketa, Naohiko

AU - Higa, Kazunari

AU - Suzuki, Terumasa

AU - Yagi-Yaguchi, Yukari

AU - Satake, Yoshiyuki

AU - Abe, Takayuki

AU - Tsubota, Kazuo

AU - Shimazaki, Jun

PY - 2018/2/1

Y1 - 2018/2/1

N2 - Purpose: To evaluate the association between endothelial cell density (ECD) after Descemet’s stripping automated endothelial keratoplasty (DSAEK) and preoperative cytokine levels in the aqueous humor (AqH). Methods: This prospective consecutive case series included 97 consecutive patients who underwent DSAEK (64 eyes) or cataract surgery (33 eyes). AqH samples were collected at the beginning of each surgery. The levels of cytokines (IL-1α, IL-1β, IL-4, IL-6, IL-8, IL-10, IL-12p70, IL-13, IL-17A, IFN-α, IFN-γ, monocyte chemotactic protein [MCP]-1, E-selectin, P-selectin, and soluble intercellular adhesion molecule [sICAM]-1) in the AqH were measured by multiplex beads immunoassay. The correlations between preoperative aqueous cytokine levels and the ECD at 12 months after DSAEK were analyzed. Results: The ECD decreased from 2747 ± 259 cells/mm2in the donor graft to 1235 ± 607 cells/mm2at 12 months after DSAEK. In all subjects undergoing DSAEK, the postoperative ECD at 12 months was significantly correlated with the preoperative levels of MCP-1 (r = −0.467, 95% confidence interval [CI]: −0.650 to −0.222, P = 0.0003). In an analysis excluding Fuchs endothelial corneal dystrophy (11 eyes), the ECD at 12 months after DSAEK was significantly correlated with preoperative levels of IL-17A (r = −0.635, 95% CI: −0.819 to −0.319, P = 0.0004), MCP-1 (r = −0.605, 95% CI: −0.779 to −0.345, P < 0.0001), IFN-γ (r = −0.633, 95% CI: −0.796 to −0.385, P < 0.0001), E-selectin (r = −0.516, 95% CI: −0.756 to −0.276, P = 0.0004), and sICAM-1 (r = −0.537, 95% CI: −0.735 to −0.253, P = 0.0005). Conclusions: Higher preoperative levels of IL-17A, MCP-1, IFN-γ, E-selectin, and sICAM-1 in the AqH were associated with lower ECD after DSAEK for bullous keratopathy.

AB - Purpose: To evaluate the association between endothelial cell density (ECD) after Descemet’s stripping automated endothelial keratoplasty (DSAEK) and preoperative cytokine levels in the aqueous humor (AqH). Methods: This prospective consecutive case series included 97 consecutive patients who underwent DSAEK (64 eyes) or cataract surgery (33 eyes). AqH samples were collected at the beginning of each surgery. The levels of cytokines (IL-1α, IL-1β, IL-4, IL-6, IL-8, IL-10, IL-12p70, IL-13, IL-17A, IFN-α, IFN-γ, monocyte chemotactic protein [MCP]-1, E-selectin, P-selectin, and soluble intercellular adhesion molecule [sICAM]-1) in the AqH were measured by multiplex beads immunoassay. The correlations between preoperative aqueous cytokine levels and the ECD at 12 months after DSAEK were analyzed. Results: The ECD decreased from 2747 ± 259 cells/mm2in the donor graft to 1235 ± 607 cells/mm2at 12 months after DSAEK. In all subjects undergoing DSAEK, the postoperative ECD at 12 months was significantly correlated with the preoperative levels of MCP-1 (r = −0.467, 95% confidence interval [CI]: −0.650 to −0.222, P = 0.0003). In an analysis excluding Fuchs endothelial corneal dystrophy (11 eyes), the ECD at 12 months after DSAEK was significantly correlated with preoperative levels of IL-17A (r = −0.635, 95% CI: −0.819 to −0.319, P = 0.0004), MCP-1 (r = −0.605, 95% CI: −0.779 to −0.345, P < 0.0001), IFN-γ (r = −0.633, 95% CI: −0.796 to −0.385, P < 0.0001), E-selectin (r = −0.516, 95% CI: −0.756 to −0.276, P = 0.0004), and sICAM-1 (r = −0.537, 95% CI: −0.735 to −0.253, P = 0.0005). Conclusions: Higher preoperative levels of IL-17A, MCP-1, IFN-γ, E-selectin, and sICAM-1 in the AqH were associated with lower ECD after DSAEK for bullous keratopathy.

KW - Aqueous humor

KW - Cytokine

KW - Descemet’s stripping automated endothelial keratoplasty

KW - Endothelial cell density

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