Preparation and biological application of fluorescent poly(methyl methacrylate) Beads Hybridized with Y3Al5O 12:Ce3+ nanophosphor

Ikumi Kusayama, Ryo Asakura, Daisuke Saito, Tetsuhiko Isobe, Kiyoshi Kurokawa, Hideki Aizawa, Tomohiro Takagi, Yosuke Hirayama, Michio Ohkubo

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Positively charged Y3Al5O12Ce3+ nanoparticles and negatively charged poly(sodium 4-styrenesulfonate) are sequentially adsorbed on the surface of negatively charged poly(methyl methacrylate) beads by the layer-by-layer adsorption method to obtain the composite beads, showing green emission under the blue light excitation. These fluorescent composite beads are detected as a dot group by flowcytometry (FCM). Bovine serum albumin (BSA) immomobilized on the composite beads with green emission are mixed with anti-BSA tagged with organic red dye. The antigen-antibody reaction is detected from both green and red emissions by FCM, showing that the fluorescent composite beads can be used for detecting biological reactions.

Original languageEnglish
Title of host publicationECS Transactions - Physics and Chemistry of Luminescent Materials, Including the 4th Symposium on Persistent Phosphors
Pages69-74
Number of pages6
Edition31
Publication statusPublished - 2009 Nov 25
EventPhysics and Chemistry of Luminescent Materials, Including the 4th Symposium on Persistent Phosphors - 214th ECS Meeting - Honolulu, HI, United States
Duration: 2008 Oct 122008 Oct 17

Publication series

NameECS Transactions
Number31
Volume16
ISSN (Print)1938-5862
ISSN (Electronic)1938-6737

Other

OtherPhysics and Chemistry of Luminescent Materials, Including the 4th Symposium on Persistent Phosphors - 214th ECS Meeting
CountryUnited States
CityHonolulu, HI
Period08/10/1208/10/17

ASJC Scopus subject areas

  • Engineering(all)

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