Preparation and characterization of DNA-lipoglutamate complexes

Toshinori Sato, T. Kawakami, N. Shirakawa, Y. Okahata

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

We newly prepared DNA-lipoglutamate polyion complexes by mixing aqueous solutions of DNA and cationic lipoglutamates such as α,γ-dibutyl glutamate (2C4N+), α,γ-dihexyl glutamate (2C6N+), and α,γ-dioctyl glutamate (2C8N+). Formation of the DNA-lipoglutamate complex was confirmed by gel chromatography, elemental analysis, CD spectra, and light scattering measurement. Compaction of DNA by binding with the cationic lipoglutamate was revealed by multi-angle light scattering. The DNA-lipoglutamate complexes showed the high stability for enzymatic hydrolysis by DNase I, which was confirmed by a quartz-crystal microbalance (QCM) technique. The DNA-lipoglutamate complexes showed the strong interactions with the lipid membranes and tumor cells compared with native DNA.

Original languageEnglish
Pages (from-to)2709-2715
Number of pages7
JournalBulletin of the Chemical Society of Japan
Volume68
Issue number9
DOIs
Publication statusPublished - 1995 Sep
Externally publishedYes

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DNA
Glutamic Acid
Light scattering
Enzymatic hydrolysis
Quartz crystal microbalances
Deoxyribonuclease I
Membrane Lipids
Chromatography
Tumors
Compaction
Gels
Cells
Chemical analysis

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Preparation and characterization of DNA-lipoglutamate complexes. / Sato, Toshinori; Kawakami, T.; Shirakawa, N.; Okahata, Y.

In: Bulletin of the Chemical Society of Japan, Vol. 68, No. 9, 09.1995, p. 2709-2715.

Research output: Contribution to journalArticle

Sato, Toshinori ; Kawakami, T. ; Shirakawa, N. ; Okahata, Y. / Preparation and characterization of DNA-lipoglutamate complexes. In: Bulletin of the Chemical Society of Japan. 1995 ; Vol. 68, No. 9. pp. 2709-2715.
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