We newly prepared DNA-lipoglutamate polyion complexes by mixing aqueous solutions of DNA and cationic lipoglutamates such as α,γ-dibutyl glutamate (2C4N+), α,γ-dihexyl glutamate (2C6N+), and α,γ-dioctyl glutamate (2C8N+). Formation of the DNA-lipoglutamate complex was confirmed by gel chromatography, elemental analysis, CD spectra, and light scattering measurement. Compaction of DNA by binding with the cationic lipoglutamate was revealed by multi-angle light scattering. The DNA-lipoglutamate complexes showed the high stability for enzymatic hydrolysis by DNase I, which was confirmed by a quartz-crystal microbalance (QCM) technique. The DNA-lipoglutamate complexes showed the strong interactions with the lipid membranes and tumor cells compared with native DNA.
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