TY - JOUR
T1 - Preparation and characterization of DNA-lipoglutamate complexes
AU - Sato, T.
AU - Kawakami, T.
AU - Shirakawa, N.
AU - Okahata, Y.
PY - 1995/9/1
Y1 - 1995/9/1
N2 - We newly prepared DNA-lipoglutamate polyion complexes by mixing aqueous solutions of DNA and cationic lipoglutamates such as α,γ-dibutyl glutamate (2C4N+), α,γ-dihexyl glutamate (2C6N+), and α,γ-dioctyl glutamate (2C8N+). Formation of the DNA-lipoglutamate complex was confirmed by gel chromatography, elemental analysis, CD spectra, and light scattering measurement. Compaction of DNA by binding with the cationic lipoglutamate was revealed by multi-angle light scattering. The DNA-lipoglutamate complexes showed the high stability for enzymatic hydrolysis by DNase I, which was confirmed by a quartz-crystal microbalance (QCM) technique. The DNA-lipoglutamate complexes showed the strong interactions with the lipid membranes and tumor cells compared with native DNA.
AB - We newly prepared DNA-lipoglutamate polyion complexes by mixing aqueous solutions of DNA and cationic lipoglutamates such as α,γ-dibutyl glutamate (2C4N+), α,γ-dihexyl glutamate (2C6N+), and α,γ-dioctyl glutamate (2C8N+). Formation of the DNA-lipoglutamate complex was confirmed by gel chromatography, elemental analysis, CD spectra, and light scattering measurement. Compaction of DNA by binding with the cationic lipoglutamate was revealed by multi-angle light scattering. The DNA-lipoglutamate complexes showed the high stability for enzymatic hydrolysis by DNase I, which was confirmed by a quartz-crystal microbalance (QCM) technique. The DNA-lipoglutamate complexes showed the strong interactions with the lipid membranes and tumor cells compared with native DNA.
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U2 - 10.1246/bcsj.68.2709
DO - 10.1246/bcsj.68.2709
M3 - Article
AN - SCOPUS:0028853753
SN - 0009-2673
VL - 68
SP - 2709
EP - 2715
JO - Bulletin of the Chemical Society of Japan
JF - Bulletin of the Chemical Society of Japan
IS - 9
ER -