Preparation and separation of metallobacteriochlorophyll a by reversed-phase high-performance liquid chromatography

H. Inoue, Y. Nonomura, A. Ashizawa, I. Tajima, M. Nomura, N. Nakamura, N. Yoshioka

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Semi-preparative high-performance liquid chromatography has been used for the preparation of copper(II) bacteriochlorophyll a [Cu(II)-BCh]-a] and zinc(II) bacteriochlorophyll a [Zn(II)-BCh]-a]. Both compounds are separated on a reversed-phase Inertsil ODS-2 column using a mobile phase of acetone-methanol (25:75, v/v). The fractionated metallobacteriochlorophylls (M-Bchl-a) are identified by fast atom bombardment mass spectrometry. The spectroscopic parameters such as the wavelength of absorption maxima and the molar extinction coefficients are determined using pure M-Bchl-a obtained by preparative HPLC. The HPLC method proposed here has been demonstrated to be useful for the purification and determination of components of M-Bchl-a.

Original languageEnglish
Pages (from-to)361-364
Number of pages4
JournalChromatographia
Volume43
Issue number7-8
DOIs
Publication statusPublished - 1996 Nov 11

Keywords

  • Bacteriochlorophyll a
  • Column liquid chromatography
  • Copper(II) bacteriochlorophyll a
  • Zinc(II) bacteriochlorophyll a

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Organic Chemistry

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