Preparation and separation of metallobacteriochlorophyll a by reversed-phase high-performance liquid chromatography

H. Inoue, Y. Nonomura, A. Ashizawa, I. Tajima, M. Nomura, N. Nakamura, Naoki Yoshioka

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Semi-preparative high-performance liquid chromatography has been used for the preparation of copper(II) bacteriochlorophyll a [Cu(II)-BCh]-a] and zinc(II) bacteriochlorophyll a [Zn(II)-BCh]-a]. Both compounds are separated on a reversed-phase Inertsil ODS-2 column using a mobile phase of acetone-methanol (25:75, v/v). The fractionated metallobacteriochlorophylls (M-Bchl-a) are identified by fast atom bombardment mass spectrometry. The spectroscopic parameters such as the wavelength of absorption maxima and the molar extinction coefficients are determined using pure M-Bchl-a obtained by preparative HPLC. The HPLC method proposed here has been demonstrated to be useful for the purification and determination of components of M-Bchl-a.

Original languageEnglish
Pages (from-to)361-364
Number of pages4
JournalChromatographia
Volume43
Issue number7-8
DOIs
Publication statusPublished - 1996

Fingerprint

Bacteriochlorophylls
High performance liquid chromatography
Reverse-Phase Chromatography
High Pressure Liquid Chromatography
Myeloma Proteins
Acetone
Fast Atom Bombardment Mass Spectrometry
Purification
Mass spectrometry
Methanol
Zinc
Copper
Wavelength
Atoms

Keywords

  • Bacteriochlorophyll a
  • Column liquid chromatography
  • Copper(II) bacteriochlorophyll a
  • Zinc(II) bacteriochlorophyll a

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry

Cite this

Preparation and separation of metallobacteriochlorophyll a by reversed-phase high-performance liquid chromatography. / Inoue, H.; Nonomura, Y.; Ashizawa, A.; Tajima, I.; Nomura, M.; Nakamura, N.; Yoshioka, Naoki.

In: Chromatographia, Vol. 43, No. 7-8, 1996, p. 361-364.

Research output: Contribution to journalArticle

Inoue, H. ; Nonomura, Y. ; Ashizawa, A. ; Tajima, I. ; Nomura, M. ; Nakamura, N. ; Yoshioka, Naoki. / Preparation and separation of metallobacteriochlorophyll a by reversed-phase high-performance liquid chromatography. In: Chromatographia. 1996 ; Vol. 43, No. 7-8. pp. 361-364.
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AU - Nakamura, N.

AU - Yoshioka, Naoki

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