Preparation of a DNA Complex with Lipoglutamide Having Tetraethylene Glycol Tails, and Its Application to DNA Delivery into Tumor Cells1

Toshinori Sato, Hiroyuki Akino, Yoshio Okahata

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We newly prepared a polyion complex of DNA with lipoglutamide, N,N′-bis(18-hydroxy-7,10,13,16-tetraoxaoctadecyl)-L-glutamide hydrochloride (abbreviated to 2EO4C6N+). Formation and characterization of the DNA/2EO4C6N+ complex were investigated by elemental analysis, melting temperature, and light-scattering measurements. Compaction of DNA by binding with the cationic 2EO4C6N+ was seen by multi-angle light scattering. Aggregation of the DNA/2EO4C6N+ complex was significantly depressed compared with the DNA complex without tetraethylene glycol. The DNA/2EO4C6N+ complex had low cytotoxicity and efficient internalization into tumor cells compared with native DNA.

Original languageEnglish
Pages (from-to)2335-2340
Number of pages6
JournalBulletin of the Chemical Society of Japan
Volume69
Issue number8
DOIs
Publication statusPublished - 1996 Aug
Externally publishedYes

    Fingerprint

ASJC Scopus subject areas

  • Chemistry(all)

Cite this