Preparation of a DNA Complex with Lipoglutamide Having Tetraethylene Glycol Tails, and Its Application to DNA Delivery into Tumor Cells1

Toshinori Sato, Hiroyuki Akino, Yoshio Okahata

Research output: Contribution to journalArticle

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Abstract

We newly prepared a polyion complex of DNA with lipoglutamide, N,N′-bis(18-hydroxy-7,10,13,16-tetraoxaoctadecyl)-L-glutamide hydrochloride (abbreviated to 2EO4C6N+). Formation and characterization of the DNA/2EO4C6N+ complex were investigated by elemental analysis, melting temperature, and light-scattering measurements. Compaction of DNA by binding with the cationic 2EO4C6N+ was seen by multi-angle light scattering. Aggregation of the DNA/2EO4C6N+ complex was significantly depressed compared with the DNA complex without tetraethylene glycol. The DNA/2EO4C6N+ complex had low cytotoxicity and efficient internalization into tumor cells compared with native DNA.

Original languageEnglish
Pages (from-to)2335-2340
Number of pages6
JournalBulletin of the Chemical Society of Japan
Volume69
Issue number8
Publication statusPublished - 1996 Aug
Externally publishedYes

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Glycols
Tumors
DNA
Light scattering
Cytotoxicity
Melting point
Compaction
Agglomeration
Cells
Chemical analysis

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Preparation of a DNA Complex with Lipoglutamide Having Tetraethylene Glycol Tails, and Its Application to DNA Delivery into Tumor Cells1 . / Sato, Toshinori; Akino, Hiroyuki; Okahata, Yoshio.

In: Bulletin of the Chemical Society of Japan, Vol. 69, No. 8, 08.1996, p. 2335-2340.

Research output: Contribution to journalArticle

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