PREPARATION OF A MONOCLONAL ANTIBODY AGAINST MOUSE BRAIN NATRIURETIC PEPTIDE (BNP) AND TISSUE DISTRIBUTION OF BNP IN MICE

Masayo Nakagawa, Issei Tanaka, Shin‐ichi ‐i Suga, Yoshihiro Ogawa, Naohisa Tamura, Masahisa Goto, Akira Sugawara, Takaaki Yoshimasa, Hiroshi Itoh, Masashi Mukoyama, Kazuwa Nakao

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

1. In order to explore the significance of brain natriuretic peptide (BNP), a cardiac hormone secreted from the ventricle, in mice, we prepared a monoclonal antibody against mouse BNP (mBNP) and established a specific radioimmunoassay (RIA) for mBNP. 2. A monoclonal antibody, KY‐mBNP‐I, was prepared by the fusion of mouse myeloma cells X63‐Ag8.653 with spleen cells of the BALB/c mouse immunized with synthetic mBNP[108–121] conjugated to bovine thyroglobulin. KY‐mBNP‐I belonged to an IgG2a subclass and showed a high affinity for mBNP(Ka = 1.8 × 1011 mol/L−l). 3. The RIA established that using KY‐mBNP‐I was highly sensitive and specific for mBNP, with an IC50 value of 3 fmol/ tube and cross‐reactivities of less than 0.003% with related natriuretic peptides. mBNP‐like immunoreactivity (mBNP‐LI) was detected in the mouse atrium (0.35 ± 0.02 nmol/g), ventricle (20.5 ± 0.5 pmol/g) and kidney (0.50 ± 0.05 pmol/g), but not in other tissues including brain. 4. Gel filtration analysis revealed that the major component of tissue mBNP‐LI was co‐eluted with synthetic mBNP[77–1211, a 45‐amino acid mature peptide. 5. The monoclonal antibody and RIA for mBNP established here will provide useful tools to investigate the functional significance of BNP in mice, coupled with the genetic engineering approach.

Original languageEnglish
Pages (from-to)S186-S187
JournalClinical and Experimental Pharmacology and Physiology
Volume22
DOIs
Publication statusPublished - 1995 Nov
Externally publishedYes

Keywords

  • atrial natriuretic peptide
  • brain natriuretic peptide
  • monoclonal antibody
  • natriuretic peptide family
  • radioimmunoassay
  • tissue distribution
  • transgenic mouse

ASJC Scopus subject areas

  • Physiology
  • Pharmacology
  • Physiology (medical)

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