Abstract
Orotate phosphoribosyl transferase (OPRT, EC 2.4.2.10) is a key enzyme in the anabolism of 5-fluorouracil (5 FU), and its expression in tumor is thought to increase the efficacy of 5-FU against the tumor. To detect the OPRT protein by immunoblotting and/or immunohistochemical methods, we tried to prepare highly specific antibody against the peptide including human OPRT amino acid sequence. The anti-OPRT polyclonal antibody obtained by immunization of OPRT peptides to rabbits had high specificity for the OPRT protein in human tumor xenografts in immunoblot and immunohistochemical analysis. Furthermore, there was a positive correlation between the activity and proteins of OPRT (R2=0.632) in 12 human tumors. These results suggest that immunohistochemical detection of tumoral OPRT protein expression using our anti-OPRT antibody may provide useful methods to predict the clinical response to 5-FU-based chemotherapy.
| Original language | English |
|---|---|
| Pages (from-to) | 653-658 |
| Number of pages | 6 |
| Journal | Gan to kagaku ryoho. Cancer & chemotherapy |
| Volume | 32 |
| Issue number | 5 |
| Publication status | Published - 2005 May |
| Externally published | Yes |
ASJC Scopus subject areas
- Oncology
- Cancer Research
