Preparation of anti-OPRT antibody for immunochemical detection

Kazuki Sakamoto, Yoshikazu Sugimoto, Kazutaka Miyadera, Toshinori Oka, Masakazu Fukushima

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Orotate phosphoribosyl transferase (OPRT, EC 2.4.2.10) is a key enzyme in the anabolism of 5-fluorouracil (5 FU), and its expression in tumor is thought to increase the efficacy of 5-FU against the tumor. To detect the OPRT protein by immunoblotting and/or immunohistochemical methods, we tried to prepare highly specific antibody against the peptide including human OPRT amino acid sequence. The anti-OPRT polyclonal antibody obtained by immunization of OPRT peptides to rabbits had high specificity for the OPRT protein in human tumor xenografts in immunoblot and immunohistochemical analysis. Furthermore, there was a positive correlation between the activity and proteins of OPRT (R2=0.632) in 12 human tumors. These results suggest that immunohistochemical detection of tumoral OPRT protein expression using our anti-OPRT antibody may provide useful methods to predict the clinical response to 5-FU-based chemotherapy.

Original languageEnglish
Pages (from-to)653-658
Number of pages6
JournalGan to kagaku ryoho. Cancer & chemotherapy
Volume32
Issue number5
Publication statusPublished - 2005 May
Externally publishedYes

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Anti-Idiotypic Antibodies
Fluorouracil
Neoplasms
Orotate Phosphoribosyltransferase
Proteins
Peptides
Antibodies
Transferases
Immunoblotting
Heterografts
Amino Acid Sequence
Immunization
Rabbits
Drug Therapy
Enzymes

ASJC Scopus subject areas

  • Cancer Research
  • Pharmacology

Cite this

Sakamoto, K., Sugimoto, Y., Miyadera, K., Oka, T., & Fukushima, M. (2005). Preparation of anti-OPRT antibody for immunochemical detection. Gan to kagaku ryoho. Cancer & chemotherapy, 32(5), 653-658.

Preparation of anti-OPRT antibody for immunochemical detection. / Sakamoto, Kazuki; Sugimoto, Yoshikazu; Miyadera, Kazutaka; Oka, Toshinori; Fukushima, Masakazu.

In: Gan to kagaku ryoho. Cancer & chemotherapy, Vol. 32, No. 5, 05.2005, p. 653-658.

Research output: Contribution to journalArticle

Sakamoto, K, Sugimoto, Y, Miyadera, K, Oka, T & Fukushima, M 2005, 'Preparation of anti-OPRT antibody for immunochemical detection', Gan to kagaku ryoho. Cancer & chemotherapy, vol. 32, no. 5, pp. 653-658.
Sakamoto, Kazuki ; Sugimoto, Yoshikazu ; Miyadera, Kazutaka ; Oka, Toshinori ; Fukushima, Masakazu. / Preparation of anti-OPRT antibody for immunochemical detection. In: Gan to kagaku ryoho. Cancer & chemotherapy. 2005 ; Vol. 32, No. 5. pp. 653-658.
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