Preparation of bionanocapsules by the layer-by-layer deposition of polypeptides onto a liposome

Keiji Fujimoto, Tomonori Toyoda, Yuuka Fukui

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Hollow nanoparticles (bionanocapsules) were prepared using liposomes as a template for the layerby-layer deposition of biopolymers. We carried out the alternative deposition of poly-L-lysine (PLys) and poly-L-aspartic acid (PAsP) onto a negatively charged liposome, which was prepared from dilauroyl phosphatidic acid (DLPA) and dimyristoylphosphatidylcholine (DMPC). Efficient separation of nanocapsules from unbound polymers was achieved by ultrafiltration. ζ-Potentials of nanocapsules changed between positive and negative charges at each deposition. FE-TEM revealed that the liposome was covered with a thin polymeric layer. A fluorescent probe, l-hydroxypyrene-3,6,8- trisulfonic acid (HPTS), was encapsulated into nanocapsules. The release of HPTS was suppressed by the polymer deposition, and the release rate was tunable by coverage of the first PLys layer and the ratio of DLPA.

Original languageEnglish
Pages (from-to)5122-5128
Number of pages7
JournalMacromolecules
Volume40
Issue number14
DOIs
Publication statusPublished - 2007 Jul 10

Fingerprint

Liposomes
Polypeptides
Nanocapsules
Peptides
Phosphatidic Acids
Acids
Lysine
Polymers
Dimyristoylphosphatidylcholine
Biopolymers
Ultrafiltration
Fluorescent Dyes
Aspartic Acid
Nanoparticles
Transmission electron microscopy

ASJC Scopus subject areas

  • Materials Chemistry

Cite this

Preparation of bionanocapsules by the layer-by-layer deposition of polypeptides onto a liposome. / Fujimoto, Keiji; Toyoda, Tomonori; Fukui, Yuuka.

In: Macromolecules, Vol. 40, No. 14, 10.07.2007, p. 5122-5128.

Research output: Contribution to journalArticle

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