Preparation of bionanocapsules by the layer-by-layer deposition of polypeptides onto a liposome

Keiji Fujimoto, Tomonori Toyoda, Yuuka Fukui

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51 Citations (Scopus)


Hollow nanoparticles (bionanocapsules) were prepared using liposomes as a template for the layerby-layer deposition of biopolymers. We carried out the alternative deposition of poly-L-lysine (PLys) and poly-L-aspartic acid (PAsP) onto a negatively charged liposome, which was prepared from dilauroyl phosphatidic acid (DLPA) and dimyristoylphosphatidylcholine (DMPC). Efficient separation of nanocapsules from unbound polymers was achieved by ultrafiltration. ζ-Potentials of nanocapsules changed between positive and negative charges at each deposition. FE-TEM revealed that the liposome was covered with a thin polymeric layer. A fluorescent probe, l-hydroxypyrene-3,6,8- trisulfonic acid (HPTS), was encapsulated into nanocapsules. The release of HPTS was suppressed by the polymer deposition, and the release rate was tunable by coverage of the first PLys layer and the ratio of DLPA.

Original languageEnglish
Pages (from-to)5122-5128
Number of pages7
Issue number14
Publication statusPublished - 2007 Jul 10


ASJC Scopus subject areas

  • Organic Chemistry
  • Polymers and Plastics
  • Inorganic Chemistry
  • Materials Chemistry

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