Preparation of conophylline affinity nano-beads and identification of a target protein

Eriko Suzuki, Hideki Ogura, Kuniki Kato, Izumi Takei, Yasuaki Kabe, Hiroshi Handa, Kazuo Umezawa

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Conophylline, a vinca alkaloid extracted from the tropical plant Ervatamia microphylla, has been shown to induce the differentiation of insulin-producing β-cells in cultured cells and in animals. However, its mechanism of action and the molecular target have remained unclear. Therefore, we prepared a fishing probe with conophylline to identify the target protein by using latex nano-beads, which are newly innovated tools for affinity-purification. With these conophylline-linked nano-beads, we found that conophylline directly interacted with ARL6IP. ARL6IP may thus be involved in the mechanism of cellular differentiation of β-cells, and this probe should be useful to find other target proteins.

Original languageEnglish
Pages (from-to)6188-6195
Number of pages8
JournalBioorganic and Medicinal Chemistry
Volume17
Issue number17
DOIs
Publication statusPublished - 2009 Sep 1
Externally publishedYes

Keywords

  • ARL6IP
  • Affinity-purification
  • Amination
  • Conophylline
  • Nano-beads

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmaceutical Science
  • Drug Discovery
  • Clinical Biochemistry
  • Organic Chemistry

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