Abstract
We have developed polypeptide-carrying microspheres for bioaffinity separation. The carrier microspheres were composed of a polystyrene core and a poly(glycidyl methacrylate) surface layer on which ethyleneglycol diglycidyl ether was introduced as a spacer. The epoxy group of the spacer's end was hydrolyzed. The hydroxy end group was then activated by tosyl(p-toluenesulfonyl) chloride. To tosyl-group-carrying microspheres, we immobilized a polypeptide, HT-Lys5-N236, which contains the carboxyl terminal region of transcription factor hGABP β, analyzed the structure of the polypeptide immobilized on the surface of microspheres and used these hybrid microspheres for the purification of N236-binding protein from HeLa cell nuclear extracts directly and from the fractions separated with a phosphocellulose column.
| Original language | English |
|---|---|
| Pages (from-to) | 41-49 |
| Number of pages | 9 |
| Journal | Colloids and Surfaces B: Biointerfaces |
| Volume | 10 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1997 Oct |
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Keywords
- Affinity purification
- Dimerization
- Microsphere
- Polypeptide
ASJC Scopus subject areas
- Biotechnology
- Colloid and Surface Chemistry
- Physical and Theoretical Chemistry
- Surfaces and Interfaces
Cite this
Preparation of dimerized polypeptide-carrying microspheres and purification of specific proteins bound to these microspheres. / Hatakeyama, M.; Ogura, Y.; Sawada, J.; Fujimoto, Keiji; Handa, H.; Kawaguchi, H.
In: Colloids and Surfaces B: Biointerfaces, Vol. 10, No. 1, 10.1997, p. 41-49.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Preparation of dimerized polypeptide-carrying microspheres and purification of specific proteins bound to these microspheres
AU - Hatakeyama, M.
AU - Ogura, Y.
AU - Sawada, J.
AU - Fujimoto, Keiji
AU - Handa, H.
AU - Kawaguchi, H.
PY - 1997/10
Y1 - 1997/10
N2 - We have developed polypeptide-carrying microspheres for bioaffinity separation. The carrier microspheres were composed of a polystyrene core and a poly(glycidyl methacrylate) surface layer on which ethyleneglycol diglycidyl ether was introduced as a spacer. The epoxy group of the spacer's end was hydrolyzed. The hydroxy end group was then activated by tosyl(p-toluenesulfonyl) chloride. To tosyl-group-carrying microspheres, we immobilized a polypeptide, HT-Lys5-N236, which contains the carboxyl terminal region of transcription factor hGABP β, analyzed the structure of the polypeptide immobilized on the surface of microspheres and used these hybrid microspheres for the purification of N236-binding protein from HeLa cell nuclear extracts directly and from the fractions separated with a phosphocellulose column.
AB - We have developed polypeptide-carrying microspheres for bioaffinity separation. The carrier microspheres were composed of a polystyrene core and a poly(glycidyl methacrylate) surface layer on which ethyleneglycol diglycidyl ether was introduced as a spacer. The epoxy group of the spacer's end was hydrolyzed. The hydroxy end group was then activated by tosyl(p-toluenesulfonyl) chloride. To tosyl-group-carrying microspheres, we immobilized a polypeptide, HT-Lys5-N236, which contains the carboxyl terminal region of transcription factor hGABP β, analyzed the structure of the polypeptide immobilized on the surface of microspheres and used these hybrid microspheres for the purification of N236-binding protein from HeLa cell nuclear extracts directly and from the fractions separated with a phosphocellulose column.
KW - Affinity purification
KW - Dimerization
KW - Microsphere
KW - Polypeptide
UR - http://www.scopus.com/inward/record.url?scp=0031592859&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031592859&partnerID=8YFLogxK
U2 - 10.1016/S0927-7765(97)00042-8
DO - 10.1016/S0927-7765(97)00042-8
M3 - Article
AN - SCOPUS:0031592859
VL - 10
SP - 41
EP - 49
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
SN - 0927-7765
IS - 1
ER -