Abstract
We have developed DNA-carrying latex particles for the separation and purification of transcription factors. These particles consist of styrene (St), glycidyl methacrylate (GMA) and divinylbenzene (DVB). It was confirmed that the ethanolamine-treated surface of these particles suffered no nonspecific adsorption of proteins. To the latex particles sequence-specific DNA oligomers were immobilized via covalent coupling. A transcription factor, E4TF3, was efficiently purified to homogeneity using the latext particles. In contrast, the purification using DNA-carrying Sepharose gel yielded poor results. Compared to DNA-carrying Sepharose gel, the latex particles exhibited several times higher efficiency in the purification of E4TF3 from the crude nuclear extract.
| Original language | English |
|---|---|
| Pages (from-to) | 293-302 |
| Number of pages | 10 |
| Journal | Journal of Biomaterials Science, Polymer Edition |
| Volume | 5 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 1994 Jan 1 |
Keywords
- DNA-carrying latex particles
- E4TF3
- Nonspecific adsorption
- Purification
- Sepharose gel
- Sequence-specific DNA
- Transcription factor
ASJC Scopus subject areas
- Biophysics
- Bioengineering
- Biomaterials
- Biomedical Engineering