Preparation of dna-carrying affinity latex and purification of transcription factors with the latex

Y. Inomata, T. Wada, H. Handa, K. Fujimoto, H. Kawaguchi

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

We have developed DNA-carrying latex particles for the separation and purification of transcription factors. These particles consist of styrene (St), glycidyl methacrylate (GMA) and divinylbenzene (DVB). It was confirmed that the ethanolamine-treated surface of these particles suffered no nonspecific adsorption of proteins. To the latex particles sequence-specific DNA oligomers were immobilized via covalent coupling. A transcription factor, E4TF3, was efficiently purified to homogeneity using the latext particles. In contrast, the purification using DNA-carrying Sepharose gel yielded poor results. Compared to DNA-carrying Sepharose gel, the latex particles exhibited several times higher efficiency in the purification of E4TF3 from the crude nuclear extract.

Original languageEnglish
Pages (from-to)293-302
Number of pages10
JournalJournal of Biomaterials Science, Polymer Edition
Volume5
Issue number4
DOIs
Publication statusPublished - 1994 Jan 1

Keywords

  • DNA-carrying latex particles
  • E4TF3
  • Nonspecific adsorption
  • Purification
  • Sepharose gel
  • Sequence-specific DNA
  • Transcription factor

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Biomedical Engineering

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