Preparation of fluorescent poly(methyl methacrylate) beads hybridized with y3Al5O12:Ce3+ nanophosphor for biological application

Ryo Asakura, Ikumi Kusayama, Daisuke Saito, Tetsuhiko Isobe, Kiyoshi Kurokawa, Yosuke Hirayama, Hideki Aizawa, Tomohiro Takagi, Michio Ohkubo

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Poly(methyl methacrylate) beads were hybridized with Y3Al 5O12:Ce3+ nanoparticles through electrostatic interactions using the layer-by-layer adsorption technique to prepare fluorescent beads for biological application. The subsequent sequential adsorption of poly(sodium 4-styrenesulfonate) and poly(allylamine hydrochloride) onto the composite beads prevented the detachment of the Y3Al 5O12:Ce3+ nanoparticles. The fluorescent beads were observed as tight dot plots by flowcytometry. Bovine serum albumin can be immobilized on the surface of the composite beads and tagged with a red dye through an antigen-antibody reaction. This result indicates that the composite beads are useful for bead assays such as flowcytometry.

Original languageEnglish
Pages (from-to)5193-5195
Number of pages3
JournalJapanese Journal of Applied Physics, Part 1: Regular Papers and Short Notes and Review Papers
Volume46
Issue number8 A
DOIs
Publication statusPublished - 2007 Aug 6

Keywords

  • Antigen-antibody reaction
  • Flowcytometry
  • Fluorescent bead
  • Glycothermal method
  • Layer-by-layer adsorption technique
  • Nanoparticle
  • Nanophosphor
  • Photoluminescence
  • Yttrium aluminum garnet

ASJC Scopus subject areas

  • Engineering(all)
  • Physics and Astronomy(all)

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