Preparation of monoclonal antibodies against brain natriuretic peptide and their application to radioimmunoassay and passive immunization

Hiroshi Itoh, Kazuwa Nakao, Masashi Mukoyama, Yoshihiko Saito, Takayuki Yamada, Gotaro Shirakami, Hiroshi Arai, Kiminori Hosoda, Shin Ichi Suga, Ikuyo Yoshida, Hiroo Imura

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3 Citations (Scopus)

Abstract

Two monoclonal antibodies (mAbs) directed toward brain natriuretic peptide (BNP), KY-BNP-I and KY-BNP-II, have been produced. Both mAbs against BNP possessed high affinities for BNP, with association constants (Ka) of 4.0 × 109 M-1 (KY-BNP-I) and 2.0 × 1010 M-1 (KY-BNP-II). With these mAbs, specific RIAs for BNP have been established. The least detectable quantities of BNP were 5 pg/tube (KY-BNP-I) and 1 pg/tube (KY-BNP-II). Cross-reactivities of α-human and rat atrial natriuretic polypeptides were less than 0.001%. These RIAs detected BNP-like immunoreactivity (BNP-LI) not only in the porcine brain but also in the canine brain, with the highest concentration in the medulla oblongata. These RIAs also detected BNP-LI in both the porcine and canine hearts and in the porcine plasma. The iv pretreatment of purified mAb[KY-BNP-II] almost completely blocked the hypotensive action of iv administered BNP in rats, with the concomitant suppression of BNP-induced increase of the plasma cyclic GMP level. These results indicate that our mAbs against BNP will serve as a useful tool for the elucidation of the physiological and pathophysiological significance of BNP as a neuropeptide and as a hormone.

Original languageEnglish
Pages (from-to)1292-1300
Number of pages9
JournalEndocrinology
Volume127
Issue number3
Publication statusPublished - 1990 Sep
Externally publishedYes

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ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Itoh, H., Nakao, K., Mukoyama, M., Saito, Y., Yamada, T., Shirakami, G., Arai, H., Hosoda, K., Suga, S. I., Yoshida, I., & Imura, H. (1990). Preparation of monoclonal antibodies against brain natriuretic peptide and their application to radioimmunoassay and passive immunization. Endocrinology, 127(3), 1292-1300.