Preparation of nanoparticles of PEG-grafted poly-L-lysine for drug delivery

Emi Kakizawa, Takuya Ishii, Keiji Fujimoto

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Drug carriers are expected to provide a controlled release and a selective targeting of drugs. At the same time, the long circulation time of carriers in blood and their degradable property are required. The secondary structure of poly-L-lysine (PLL) changed responding to pHs and temperatures among random, α -helix, and β -sheet forms. Poly(ethylene glycol) (PEG) was coupled with the backbone of PLL (mPEG-PLL graft polymers). PEG-rich graft polymers were associated to form nanoparticles spontaneously by the formation of β -sheet structures when both pHs and temperatures were raised. Nanoparticles were cross-linked with divinyl sulfone (DVS) to prevent them from dissolving. By circular dichroism (CD) spectrometry, it was found that the cross-linked nanoparticles changed their secondary structure from β -sheet to ramdom coil and thereby swelled and became soluble partially as the pH was lowered to 6.0. This suggests that cross-linked nanoparticles have an ability to release drugs in response to pH when they are taken up by cells and delivered to endosomes.

Original languageEnglish
Title of host publicationPolymer Preprints, Japan
Pages2351
Number of pages1
Volume54
Edition1
Publication statusPublished - 2005
Event54th SPSJ Annual Meeting 2005 - Yokohama, Japan
Duration: 2005 May 252005 May 27

Other

Other54th SPSJ Annual Meeting 2005
CountryJapan
CityYokohama
Period05/5/2505/5/27

Fingerprint

Drug delivery
Polyethylene glycols
Nanoparticles
Graft copolymers
Dichroism
Spectrometry
Blood
Temperature

Keywords

  • Beta-sheet
  • Drug delivery
  • Graft polymer
  • Nanoparticle
  • Poly(ethylene glycol) (PEG)
  • Poly-L-lysine (PLL)

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Kakizawa, E., Ishii, T., & Fujimoto, K. (2005). Preparation of nanoparticles of PEG-grafted poly-L-lysine for drug delivery. In Polymer Preprints, Japan (1 ed., Vol. 54, pp. 2351)

Preparation of nanoparticles of PEG-grafted poly-L-lysine for drug delivery. / Kakizawa, Emi; Ishii, Takuya; Fujimoto, Keiji.

Polymer Preprints, Japan. Vol. 54 1. ed. 2005. p. 2351.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Kakizawa, E, Ishii, T & Fujimoto, K 2005, Preparation of nanoparticles of PEG-grafted poly-L-lysine for drug delivery. in Polymer Preprints, Japan. 1 edn, vol. 54, pp. 2351, 54th SPSJ Annual Meeting 2005, Yokohama, Japan, 05/5/25.
Kakizawa E, Ishii T, Fujimoto K. Preparation of nanoparticles of PEG-grafted poly-L-lysine for drug delivery. In Polymer Preprints, Japan. 1 ed. Vol. 54. 2005. p. 2351
Kakizawa, Emi ; Ishii, Takuya ; Fujimoto, Keiji. / Preparation of nanoparticles of PEG-grafted poly-L-lysine for drug delivery. Polymer Preprints, Japan. Vol. 54 1. ed. 2005. pp. 2351
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