Preparation of nanoparticles of PEG-grafted poly-L-lysine for drug delivery

Emi Kakizawa, Takuya Ishii, Keiji Fujimoto

Research output: Contribution to conferencePaper

Abstract

Drug carriers are expected to provide a controlled release and a selective targeting of drugs. At the same time, the long circulation time of carriers in blood and their degradable property are required. The secondary structure of poly-L-lysine (PLL) changed responding to pHs and temperatures among random, α -helix, and β -sheet forms. Poly(ethylene glycol) (PEG) was coupled with the backbone of PLL (mPEG-PLL graft polymers). PEG-rich graft polymers were associated to form nanoparticles spontaneously by the formation of β -sheet structures when both pHs and temperatures were raised. Nanoparticles were cross-linked with divinyl sulfone (DVS) to prevent them from dissolving. By circular dichroism (CD) spectrometry, it was found that the cross-linked nanoparticles changed their secondary structure from β -sheet to ramdom coil and thereby swelled and became soluble partially as the pH was lowered to 6.0. This suggests that cross-linked nanoparticles have an ability to release drugs in response to pH when they are taken up by cells and delivered to endosomes.

Original languageEnglish
Number of pages1
Publication statusPublished - 2005 Dec 1
Event54th SPSJ Annual Meeting 2005 - Yokohama, Japan
Duration: 2005 May 252005 May 27

Other

Other54th SPSJ Annual Meeting 2005
CountryJapan
CityYokohama
Period05/5/2505/5/27

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Keywords

  • Beta-sheet
  • Drug delivery
  • Graft polymer
  • Nanoparticle
  • Poly(ethylene glycol) (PEG)
  • Poly-L-lysine (PLL)

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Kakizawa, E., Ishii, T., & Fujimoto, K. (2005). Preparation of nanoparticles of PEG-grafted poly-L-lysine for drug delivery. Paper presented at 54th SPSJ Annual Meeting 2005, Yokohama, Japan.