Preparation of octyl β-D-xylobioside and xyloside by xylanase-catalyzed direct transglycosylation reaction of xylan and octanol

Shuichi Matsumura, Kazuya Sakiyama, Kazunobu Toshima

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

1-Octyl β-D-xylobioside and xyloside were prepared by direct transglycosylation reaction of xylan and 1-octanol using purified xylanase from Aureobasidium pullulans. 2-Ethylhexyl β-D-xylobioside and xyloside were also prepared in the same way. The maximum yields of 2-ethylhexyl β-D-xylobioside and 2-ethylhexyl β-D-xyloside were 110 and 54 mg/g xylan, respectively. The proposed mechanism for production of octyl xylobioside and xyloside involved the reaction of xylan and octanol by xylanase to produce octyl xylobioside and xylotrioside, the latter of which was simultaneously hydrolyzed by xylanase into octyl xyloside and xylobiose.

Original languageEnglish
Pages (from-to)17-22
Number of pages6
JournalBiotechnology Letters
Volume21
Issue number1
DOIs
Publication statusPublished - 1999 Jan 1

Keywords

  • Alkly
  • Alkyl xyloside
  • Enzymatic synthesis
  • Xylan
  • Xylanase
  • Xylobioside

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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