Preparation Of Peptide-Carrying Microspheres With Bioactivity On Platelets

Yuji Kasuya, Keiji Fujimoto, Haruma Kawaguchi, Masaki Miyamoto, Takeo Juji, Akira Otaka, Susumu Funakoshi, Nobutaka Fujii

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

A tetrapeptide RGDS (Arg-Gly-Asp-Ser), which is one of the active sites of cell adhesive proteins, was synthesized according to the solution method and covalently coupled onto monodisperse microspheres. The biospecific activity of the RGDS-carrying microspheres was investigated by measuring the stimulatory effect of the microspheres on platelets. Large and tightly packed platelet aggregation accompanied with drastic ATP release was induced by the RGDS-carrying microspheres. It was confirmed that the specific platelet aggregation was attributed to the activity of the immobilized RGDS by comparison with those induced by other microspheres.

Original languageEnglish
Pages (from-to)369-380
Number of pages12
JournalJournal of Biomaterials Science, Polymer Edition
Volume4
Issue number4
DOIs
Publication statusPublished - 1993 Jan 1

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Keywords

  • Microsphere
  • RGDS
  • biospecific activity
  • cell adhesion
  • platelet aggregation

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Biomedical Engineering

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