Preparation of peptide-carrying microspheres with bioactivity on platelets

Yuji Kasuya, Keiji Fujimoto, Haruma Kawaguchi, Masaki Miyamoto, Takeo Juji, Akira Otaka, Susumu Funakoshi, Nobutaka Fujii

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

A tetrapeptide RGDS (Arg-Gly-Asp-Ser), which is one of the active sites of cell adhesive proteins, was synthesized according to the solution method and covalently coupled onto monodisperse microspheres. The biospecific activity of the RGDS-carrying microspheres was investigated by measuring the stimulatory effect of the microspheres on platelets. Large and tightly packed platelet aggregation accompanied with drastic ATP release was induced by the RGDS-carrying microspheres. It was confirmed that the specific platelet aggregation was attributed to the activity of the immobilized RGDS by comparison with those induced by other microspheres.

Original languageEnglish
Pages (from-to)369-380
Number of pages12
JournalJournal of Biomaterials Science, Polymer Edition
Volume4
Issue number3
DOIs
Publication statusPublished - 1993

Fingerprint

Platelets
Bioactivity
Microspheres
Peptides
Blood Platelets
Platelet Aggregation
arginyl-glycyl-aspartyl-serine
Agglomeration
Adenosinetriphosphate
Adhesives
Catalytic Domain
Adenosine Triphosphate
Proteins

Keywords

  • biospecific activity
  • cell adhesion
  • Microsphere
  • platelet aggregation
  • RGDS

ASJC Scopus subject areas

  • Biophysics
  • Biomaterials
  • Biomedical Engineering
  • Bioengineering

Cite this

Preparation of peptide-carrying microspheres with bioactivity on platelets. / Kasuya, Yuji; Fujimoto, Keiji; Kawaguchi, Haruma; Miyamoto, Masaki; Juji, Takeo; Otaka, Akira; Funakoshi, Susumu; Fujii, Nobutaka.

In: Journal of Biomaterials Science, Polymer Edition, Vol. 4, No. 3, 1993, p. 369-380.

Research output: Contribution to journalArticle

Kasuya, Y, Fujimoto, K, Kawaguchi, H, Miyamoto, M, Juji, T, Otaka, A, Funakoshi, S & Fujii, N 1993, 'Preparation of peptide-carrying microspheres with bioactivity on platelets', Journal of Biomaterials Science, Polymer Edition, vol. 4, no. 3, pp. 369-380. https://doi.org/10.1163/156856293X00636
Kasuya, Yuji ; Fujimoto, Keiji ; Kawaguchi, Haruma ; Miyamoto, Masaki ; Juji, Takeo ; Otaka, Akira ; Funakoshi, Susumu ; Fujii, Nobutaka. / Preparation of peptide-carrying microspheres with bioactivity on platelets. In: Journal of Biomaterials Science, Polymer Edition. 1993 ; Vol. 4, No. 3. pp. 369-380.
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