Abstract
Poly(N-isopropylacrylamide-co-N-tert-butylacrylamide) [P(IPAAm-co-tBAAm)] brushes were prepared on poly(hydroxy methacrylate) (PHMA) [hydrolyzed poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate)] beads having large pores by surface-initiated atom transfer radical polymerization (ATRP) and applied to the stationary phases of thermo-responsive chromatography. Optimized amount of copolymer brushes grafted PHMA beads were able to separate peptides and proteins with narrow peaks and a high resolution. The beads were found to have a specific surface area of 43.0m2/g by nitrogen gas adsorption method. Copolymer brush of P(IPAAm-co-tBAAm) grafted PHMA beads improved the stationary phase of thermo-responsive chromatography for the all-aqueous separation of peptides and proteins.
| Original language | English |
|---|---|
| Pages (from-to) | 5978-5985 |
| Number of pages | 8 |
| Journal | Journal of Chromatography A |
| Volume | 1217 |
| Issue number | 38 |
| DOIs | |
| Publication status | Published - 2010 Sep |
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Keywords
- Atom transfer radical polymerization
- Poly(N-isopropylacrylamide)
- Polymer brush
- Thermo-responsive chromatography
- Thermo-responsive polymer
ASJC Scopus subject areas
- Analytical Chemistry
- Organic Chemistry
- Biochemistry
Cite this
Preparation of thermo-responsive polymer brushes on hydrophilic polymeric beads by surface-initiated atom transfer radical polymerization for a highly resolutive separation of peptides. / Mizutani, Aya; Nagase, Kenichi; Kikuchi, Akihiko; Kanazawa, Hideko; Akiyama, Yoshikatsu; Kobayashi, Jun; Annaka, Masahiko; Okano, Teruo.
In: Journal of Chromatography A, Vol. 1217, No. 38, 09.2010, p. 5978-5985.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Preparation of thermo-responsive polymer brushes on hydrophilic polymeric beads by surface-initiated atom transfer radical polymerization for a highly resolutive separation of peptides
AU - Mizutani, Aya
AU - Nagase, Kenichi
AU - Kikuchi, Akihiko
AU - Kanazawa, Hideko
AU - Akiyama, Yoshikatsu
AU - Kobayashi, Jun
AU - Annaka, Masahiko
AU - Okano, Teruo
PY - 2010/9
Y1 - 2010/9
N2 - Poly(N-isopropylacrylamide-co-N-tert-butylacrylamide) [P(IPAAm-co-tBAAm)] brushes were prepared on poly(hydroxy methacrylate) (PHMA) [hydrolyzed poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate)] beads having large pores by surface-initiated atom transfer radical polymerization (ATRP) and applied to the stationary phases of thermo-responsive chromatography. Optimized amount of copolymer brushes grafted PHMA beads were able to separate peptides and proteins with narrow peaks and a high resolution. The beads were found to have a specific surface area of 43.0m2/g by nitrogen gas adsorption method. Copolymer brush of P(IPAAm-co-tBAAm) grafted PHMA beads improved the stationary phase of thermo-responsive chromatography for the all-aqueous separation of peptides and proteins.
AB - Poly(N-isopropylacrylamide-co-N-tert-butylacrylamide) [P(IPAAm-co-tBAAm)] brushes were prepared on poly(hydroxy methacrylate) (PHMA) [hydrolyzed poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate)] beads having large pores by surface-initiated atom transfer radical polymerization (ATRP) and applied to the stationary phases of thermo-responsive chromatography. Optimized amount of copolymer brushes grafted PHMA beads were able to separate peptides and proteins with narrow peaks and a high resolution. The beads were found to have a specific surface area of 43.0m2/g by nitrogen gas adsorption method. Copolymer brush of P(IPAAm-co-tBAAm) grafted PHMA beads improved the stationary phase of thermo-responsive chromatography for the all-aqueous separation of peptides and proteins.
KW - Atom transfer radical polymerization
KW - Poly(N-isopropylacrylamide)
KW - Polymer brush
KW - Thermo-responsive chromatography
KW - Thermo-responsive polymer
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UR - http://www.scopus.com/inward/citedby.url?scp=77956170124&partnerID=8YFLogxK
U2 - 10.1016/j.chroma.2010.07.067
DO - 10.1016/j.chroma.2010.07.067
M3 - Article
VL - 1217
SP - 5978
EP - 5985
JO - Journal of Chromatography
JF - Journal of Chromatography
SN - 0021-9673
IS - 38
ER -