Preparation of thermo-responsive polymer brushes on hydrophilic polymeric beads by surface-initiated atom transfer radical polymerization for a highly resolutive separation of peptides

Aya Mizutani; Kenichi Nagase; Akihiko Kikuchi; Hideko Kanazawa; Yoshikatsu Akiyama; Jun Kobayashi; Masahiko Annaka; Teruo Okano

doi:10.1016/j.chroma.2010.07.067

Preparation of thermo-responsive polymer brushes on hydrophilic polymeric beads by surface-initiated atom transfer radical polymerization for a highly resolutive separation of peptides

Aya Mizutani, Kenichi Nagase, Akihiko Kikuchi, Hideko Kanazawa, Yoshikatsu Akiyama, Jun Kobayashi, Masahiko Annaka, Teruo Okano

School of Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

37 Citations (Scopus)

Abstract

Poly(N-isopropylacrylamide-co-N-tert-butylacrylamide) [P(IPAAm-co-tBAAm)] brushes were prepared on poly(hydroxy methacrylate) (PHMA) [hydrolyzed poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate)] beads having large pores by surface-initiated atom transfer radical polymerization (ATRP) and applied to the stationary phases of thermo-responsive chromatography. Optimized amount of copolymer brushes grafted PHMA beads were able to separate peptides and proteins with narrow peaks and a high resolution. The beads were found to have a specific surface area of 43.0m²/g by nitrogen gas adsorption method. Copolymer brush of P(IPAAm-co-tBAAm) grafted PHMA beads improved the stationary phase of thermo-responsive chromatography for the all-aqueous separation of peptides and proteins.

Original language	English
Pages (from-to)	5978-5985
Number of pages	8
Journal	Journal of Chromatography A
Volume	1217
Issue number	38
DOIs	https://doi.org/10.1016/j.chroma.2010.07.067
Publication status	Published - 2010 Sep

Keywords

Atom transfer radical polymerization
Poly(N-isopropylacrylamide)
Polymer brush
Thermo-responsive chromatography
Thermo-responsive polymer

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Organic Chemistry

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Mizutani, A., Nagase, K., Kikuchi, A., Kanazawa, H., Akiyama, Y., Kobayashi, J., Annaka, M., & Okano, T. (2010). Preparation of thermo-responsive polymer brushes on hydrophilic polymeric beads by surface-initiated atom transfer radical polymerization for a highly resolutive separation of peptides. Journal of Chromatography A, 1217(38), 5978-5985. https://doi.org/10.1016/j.chroma.2010.07.067

Preparation of thermo-responsive polymer brushes on hydrophilic polymeric beads by surface-initiated atom transfer radical polymerization for a highly resolutive separation of peptides. / Mizutani, Aya; Nagase, Kenichi; Kikuchi, Akihiko; Kanazawa, Hideko; Akiyama, Yoshikatsu; Kobayashi, Jun; Annaka, Masahiko; Okano, Teruo.

In: Journal of Chromatography A, Vol. 1217, No. 38, 09.2010, p. 5978-5985.

Research output: Contribution to journal › Article › peer-review

Mizutani, A, Nagase, K, Kikuchi, A, Kanazawa, H, Akiyama, Y, Kobayashi, J, Annaka, M & Okano, T 2010, 'Preparation of thermo-responsive polymer brushes on hydrophilic polymeric beads by surface-initiated atom transfer radical polymerization for a highly resolutive separation of peptides', Journal of Chromatography A, vol. 1217, no. 38, pp. 5978-5985. https://doi.org/10.1016/j.chroma.2010.07.067

Mizutani, Aya ; Nagase, Kenichi ; Kikuchi, Akihiko ; Kanazawa, Hideko ; Akiyama, Yoshikatsu ; Kobayashi, Jun ; Annaka, Masahiko ; Okano, Teruo. / Preparation of thermo-responsive polymer brushes on hydrophilic polymeric beads by surface-initiated atom transfer radical polymerization for a highly resolutive separation of peptides. In: Journal of Chromatography A. 2010 ; Vol. 1217, No. 38. pp. 5978-5985.

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abstract = "Poly(N-isopropylacrylamide-co-N-tert-butylacrylamide) [P(IPAAm-co-tBAAm)] brushes were prepared on poly(hydroxy methacrylate) (PHMA) [hydrolyzed poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate)] beads having large pores by surface-initiated atom transfer radical polymerization (ATRP) and applied to the stationary phases of thermo-responsive chromatography. Optimized amount of copolymer brushes grafted PHMA beads were able to separate peptides and proteins with narrow peaks and a high resolution. The beads were found to have a specific surface area of 43.0m2/g by nitrogen gas adsorption method. Copolymer brush of P(IPAAm-co-tBAAm) grafted PHMA beads improved the stationary phase of thermo-responsive chromatography for the all-aqueous separation of peptides and proteins.",

keywords = "Atom transfer radical polymerization, Poly(N-isopropylacrylamide), Polymer brush, Thermo-responsive chromatography, Thermo-responsive polymer",

author = "Aya Mizutani and Kenichi Nagase and Akihiko Kikuchi and Hideko Kanazawa and Yoshikatsu Akiyama and Jun Kobayashi and Masahiko Annaka and Teruo Okano",

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AU - Nagase, Kenichi

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AU - Kanazawa, Hideko

AU - Akiyama, Yoshikatsu

AU - Kobayashi, Jun

AU - Annaka, Masahiko

AU - Okano, Teruo

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AB - Poly(N-isopropylacrylamide-co-N-tert-butylacrylamide) [P(IPAAm-co-tBAAm)] brushes were prepared on poly(hydroxy methacrylate) (PHMA) [hydrolyzed poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate)] beads having large pores by surface-initiated atom transfer radical polymerization (ATRP) and applied to the stationary phases of thermo-responsive chromatography. Optimized amount of copolymer brushes grafted PHMA beads were able to separate peptides and proteins with narrow peaks and a high resolution. The beads were found to have a specific surface area of 43.0m2/g by nitrogen gas adsorption method. Copolymer brush of P(IPAAm-co-tBAAm) grafted PHMA beads improved the stationary phase of thermo-responsive chromatography for the all-aqueous separation of peptides and proteins.

KW - Atom transfer radical polymerization

KW - Poly(N-isopropylacrylamide)

KW - Polymer brush

KW - Thermo-responsive chromatography

KW - Thermo-responsive polymer

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