TY - JOUR
T1 - Preparation of thermo-responsive polymer brushes on hydrophilic polymeric beads by surface-initiated atom transfer radical polymerization for a highly resolutive separation of peptides
AU - Mizutani, Aya
AU - Nagase, Kenichi
AU - Kikuchi, Akihiko
AU - Kanazawa, Hideko
AU - Akiyama, Yoshikatsu
AU - Kobayashi, Jun
AU - Annaka, Masahiko
AU - Okano, Teruo
PY - 2010/9/1
Y1 - 2010/9/1
N2 - Poly(N-isopropylacrylamide-co-N-tert-butylacrylamide) [P(IPAAm-co-tBAAm)] brushes were prepared on poly(hydroxy methacrylate) (PHMA) [hydrolyzed poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate)] beads having large pores by surface-initiated atom transfer radical polymerization (ATRP) and applied to the stationary phases of thermo-responsive chromatography. Optimized amount of copolymer brushes grafted PHMA beads were able to separate peptides and proteins with narrow peaks and a high resolution. The beads were found to have a specific surface area of 43.0m2/g by nitrogen gas adsorption method. Copolymer brush of P(IPAAm-co-tBAAm) grafted PHMA beads improved the stationary phase of thermo-responsive chromatography for the all-aqueous separation of peptides and proteins.
AB - Poly(N-isopropylacrylamide-co-N-tert-butylacrylamide) [P(IPAAm-co-tBAAm)] brushes were prepared on poly(hydroxy methacrylate) (PHMA) [hydrolyzed poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate)] beads having large pores by surface-initiated atom transfer radical polymerization (ATRP) and applied to the stationary phases of thermo-responsive chromatography. Optimized amount of copolymer brushes grafted PHMA beads were able to separate peptides and proteins with narrow peaks and a high resolution. The beads were found to have a specific surface area of 43.0m2/g by nitrogen gas adsorption method. Copolymer brush of P(IPAAm-co-tBAAm) grafted PHMA beads improved the stationary phase of thermo-responsive chromatography for the all-aqueous separation of peptides and proteins.
KW - Atom transfer radical polymerization
KW - Poly(N-isopropylacrylamide)
KW - Polymer brush
KW - Thermo-responsive chromatography
KW - Thermo-responsive polymer
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U2 - 10.1016/j.chroma.2010.07.067
DO - 10.1016/j.chroma.2010.07.067
M3 - Article
C2 - 20723903
AN - SCOPUS:77956170124
VL - 1217
SP - 5978
EP - 5985
JO - Journal of Chromatography A
JF - Journal of Chromatography A
SN - 0021-9673
IS - 38
ER -