TY - JOUR
T1 - Preparation of thermo-responsive polymer brushes on hydrophilic polymeric beads by surface-initiated atom transfer radical polymerization for a highly resolutive separation of peptides
AU - Mizutani, Aya
AU - Nagase, Kenichi
AU - Kikuchi, Akihiko
AU - Kanazawa, Hideko
AU - Akiyama, Yoshikatsu
AU - Kobayashi, Jun
AU - Annaka, Masahiko
AU - Okano, Teruo
N1 - Funding Information:
Present research was supported in part by the Development of New Environmental Technology Using Nanotechnology Project of the National Institute of Environmental Science (NIES), commissioned from the Ministry of Environment, Japan , and Grants-in-Aid for Scientific Research (B) No. 20300169 from the Japan Society for the Promotion of Science . The authors are grateful to Dr. Norio Ueno, Tokyo Women's Medical University for his English editing.
PY - 2010/9
Y1 - 2010/9
N2 - Poly(N-isopropylacrylamide-co-N-tert-butylacrylamide) [P(IPAAm-co-tBAAm)] brushes were prepared on poly(hydroxy methacrylate) (PHMA) [hydrolyzed poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate)] beads having large pores by surface-initiated atom transfer radical polymerization (ATRP) and applied to the stationary phases of thermo-responsive chromatography. Optimized amount of copolymer brushes grafted PHMA beads were able to separate peptides and proteins with narrow peaks and a high resolution. The beads were found to have a specific surface area of 43.0m2/g by nitrogen gas adsorption method. Copolymer brush of P(IPAAm-co-tBAAm) grafted PHMA beads improved the stationary phase of thermo-responsive chromatography for the all-aqueous separation of peptides and proteins.
AB - Poly(N-isopropylacrylamide-co-N-tert-butylacrylamide) [P(IPAAm-co-tBAAm)] brushes were prepared on poly(hydroxy methacrylate) (PHMA) [hydrolyzed poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate)] beads having large pores by surface-initiated atom transfer radical polymerization (ATRP) and applied to the stationary phases of thermo-responsive chromatography. Optimized amount of copolymer brushes grafted PHMA beads were able to separate peptides and proteins with narrow peaks and a high resolution. The beads were found to have a specific surface area of 43.0m2/g by nitrogen gas adsorption method. Copolymer brush of P(IPAAm-co-tBAAm) grafted PHMA beads improved the stationary phase of thermo-responsive chromatography for the all-aqueous separation of peptides and proteins.
KW - Atom transfer radical polymerization
KW - Poly(N-isopropylacrylamide)
KW - Polymer brush
KW - Thermo-responsive chromatography
KW - Thermo-responsive polymer
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U2 - 10.1016/j.chroma.2010.07.067
DO - 10.1016/j.chroma.2010.07.067
M3 - Article
C2 - 20723903
AN - SCOPUS:77956170124
VL - 1217
SP - 5978
EP - 5985
JO - Journal of Chromatography A
JF - Journal of Chromatography A
SN - 0021-9673
IS - 38
ER -