Preparation of thermo-responsive polymer brushes on hydrophilic polymeric beads by surface-initiated atom transfer radical polymerization for a highly resolutive separation of peptides

Aya Mizutani, Kenichi Nagase, Akihiko Kikuchi, Hideko Kanazawa, Yoshikatsu Akiyama, Jun Kobayashi, Masahiko Annaka, Teruo Okano

Research output: Contribution to journalArticle

35 Citations (Scopus)


Poly(N-isopropylacrylamide-co-N-tert-butylacrylamide) [P(IPAAm-co-tBAAm)] brushes were prepared on poly(hydroxy methacrylate) (PHMA) [hydrolyzed poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate)] beads having large pores by surface-initiated atom transfer radical polymerization (ATRP) and applied to the stationary phases of thermo-responsive chromatography. Optimized amount of copolymer brushes grafted PHMA beads were able to separate peptides and proteins with narrow peaks and a high resolution. The beads were found to have a specific surface area of 43.0m2/g by nitrogen gas adsorption method. Copolymer brush of P(IPAAm-co-tBAAm) grafted PHMA beads improved the stationary phase of thermo-responsive chromatography for the all-aqueous separation of peptides and proteins.

Original languageEnglish
Pages (from-to)5978-5985
Number of pages8
JournalJournal of Chromatography A
Issue number38
Publication statusPublished - 2010 Sep 1



  • Atom transfer radical polymerization
  • Poly(N-isopropylacrylamide)
  • Polymer brush
  • Thermo-responsive chromatography
  • Thermo-responsive polymer

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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