Preparation of thermoresponsive polymer brush surfaces and their interaction with cells

Aya Mizutani, Akihiko Kikuchi, Masayuki Yamato, Hideko Kanazawa, Teruo Okano

Research output: Contribution to journalArticle

226 Citations (Scopus)

Abstract

Poly(N-isopropylacrylamide) (PIPAAm) brush surfaces with different layer thickness on polystyrene substrates were prepared by surface-initiated atom transfer radical polymerization (ATRP). Surface characteristics of PIPAAm brushes and their influence on adhesion and detachment of bovine carotid artery endothelial cells (ECs) were controlled by PIPAAm layer thickness. Surface hydrophilicity increased with PIPAAm layer thickness at 37 °C because PIPAAm brush surfaces with higher thickness provide more extended chain conformations with relatively high chain mobility, and accompanying polymer chain hydration. These surface property alterations lead to negligible cell adhesion through minimal matrix protein adsorption and also modified surface modulus. By adjusting polymerization reaction conditions and time, polymer layers supporting confluent cultures of ECs were possible. Confluent EC monolayers spontaneously detached as contiguous cell sheets from PIPAAm brush surfaces at reduced temperatures. Thermoresponsive cell adhesion and detachment behavior were analyzed from the standpoint of surface physicochemical characteristics. Thermoresponsive surfaces prepared by surface-initiated ATRP techniques allow surface selection in preparing cell sheets from attachment-dependent cells having relatively strong adhesive property for tissue engineering applications.

Original languageEnglish
Pages (from-to)2073-2081
Number of pages9
JournalBiomaterials
Volume29
Issue number13
DOIs
Publication statusPublished - 2008 May

Fingerprint

Brushes
Cell Communication
Polymers
Polymerization
Endothelial Cells
Cell Adhesion
Endothelial cells
Atom transfer radical polymerization
Cell adhesion
Surface Properties
Polystyrenes
Tissue Engineering
Hydrophobic and Hydrophilic Interactions
Carotid Arteries
Adhesives
Adsorption
poly-N-isopropylacrylamide
Temperature
Hydrophilicity
Tissue engineering

Keywords

  • Atom transfer radical polymerization
  • Cell culture
  • Poly(N-isopropylacrylamide)
  • Polystyrene substrates
  • Thermoresponsive polymer

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

Cite this

Preparation of thermoresponsive polymer brush surfaces and their interaction with cells. / Mizutani, Aya; Kikuchi, Akihiko; Yamato, Masayuki; Kanazawa, Hideko; Okano, Teruo.

In: Biomaterials, Vol. 29, No. 13, 05.2008, p. 2073-2081.

Research output: Contribution to journalArticle

Mizutani, Aya ; Kikuchi, Akihiko ; Yamato, Masayuki ; Kanazawa, Hideko ; Okano, Teruo. / Preparation of thermoresponsive polymer brush surfaces and their interaction with cells. In: Biomaterials. 2008 ; Vol. 29, No. 13. pp. 2073-2081.
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