Preparation of unsymmetrical microspheres at the interfaces

Keiji Fujimoto, Kazumichi Nakahama, Miwako Shidara, Haruma Kawaguchi

Research output: Contribution to journalArticle

120 Citations (Scopus)

Abstract

Many synthetic microspheres are spherical and symmetrical because of the thermodynamical limitations of the reaction systems. We developed modification techniques to prepare spherical microspheres with intrinsic unsymmetry, for example, a microsphere with both an anionic and a cationic part. The modification was performed at the liquid-solid interface. Reactive microspheres with p-nitrophenyl moieties were settled onto the IgG-preadsorbed substrate, and the reaction between the activated ester moieties and IgG molecules proceeded only at the interface upon attachment. After the reaction, the modified microspheres were detached from the substrate with ultrasonication. In another modification at the air-water interface, the dispersion of reactive microspheres in ethanol was first spread on a water surface to produce the monolayer of microspheres, and then hydrolysis at the water side of the monolayer was performed by adding a NaOH aqueous solution to the subphase. After this unsymmetrical hydrolysis, we carried out IgG immobilization with the remaining reactive moieties. In both interface methods, the immobilized IgG molecules were labeled with colloidal gold particles conjugated with anti-IgG antibodies. Transmission electron microscopy indicated that gold particles could be observed on only the immobilized side. Small microspheres were coupled to the reactive microsphere, and its unsymmetry was confirmed by scanning electron microscopy. Such unsymmetrical microspheres will be applicable to the electrical rheology, diagnosis, display technology, and creation of functional devices through the assembling of microspheres.

Original languageEnglish
Pages (from-to)4630-4635
Number of pages6
JournalLangmuir
Volume15
Issue number13
DOIs
Publication statusPublished - 1999

Fingerprint

Microspheres
preparation
hydrolysis
gold
liquid-solid interfaces
assembling
surface water
antibodies
immobilization
rheology
water
attachment
esters
molecules
Immunoglobulin G
ethyl alcohol
aqueous solutions
transmission electron microscopy
scanning electron microscopy
air

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

Cite this

Fujimoto, K., Nakahama, K., Shidara, M., & Kawaguchi, H. (1999). Preparation of unsymmetrical microspheres at the interfaces. Langmuir, 15(13), 4630-4635. https://doi.org/10.1021/la990023v

Preparation of unsymmetrical microspheres at the interfaces. / Fujimoto, Keiji; Nakahama, Kazumichi; Shidara, Miwako; Kawaguchi, Haruma.

In: Langmuir, Vol. 15, No. 13, 1999, p. 4630-4635.

Research output: Contribution to journalArticle

Fujimoto, K, Nakahama, K, Shidara, M & Kawaguchi, H 1999, 'Preparation of unsymmetrical microspheres at the interfaces', Langmuir, vol. 15, no. 13, pp. 4630-4635. https://doi.org/10.1021/la990023v
Fujimoto, Keiji ; Nakahama, Kazumichi ; Shidara, Miwako ; Kawaguchi, Haruma. / Preparation of unsymmetrical microspheres at the interfaces. In: Langmuir. 1999 ; Vol. 15, No. 13. pp. 4630-4635.
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