Presence and physiologic function of the renin-angiotensin system in mouse lacrimal gland

Saori Yaguchi, Yoko Ogawa, Shigeto Shimmura, Shin Hatou, Shigeru Nakamura, Takaaki Inaba, Toshihiro Imada, Yoko Ozawa, Yutaka Kawakami, Susumu Ishida, Kazuo Tsubota

Research output: Contribution to journalArticle

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Abstract

PURPOSE. To investigate the expression, localization, and physiologic function of renin-angiotensin system (RAS) components in the mouse lacrimal gland. METHODS. Lacrimal glands and cultured lacrimal gland fibroblasts from wild-type (WT) BALB/c (H-2d) mice were used. Reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry were used to determine the expression and localization of the RAS components, prorenin/renin, angiotensin-converting enzyme (ACE), angiotensin II, angiotensin II type 1 receptor (AT1R), and angiotensin II type 2 receptor (AT2R) in the normal mouse lacrimal gland. To examine the change in tear secretion, mice received ARB (AT1R blocker) or AT2R antagonist. Tear secretion was assessed by cotton thread test before and after drug administration. RESULTS. The mRNAs coding for angiotensinogen, prorenin, ACE, and both AT1R and AT2R were found in normal lacrimal gland tissue and cultured lacrimal gland fibroblasts. Prorenin/renin and ACE were identified in myoepithelial cells around ducts and acini and in blood vessels. Angiotensin II, AT1R, and AT2R were observed in the ducts and interstitial fibroblasts. AT1R and AT2R were also localized in blood vessels. All the cultured lacrimal gland fibroblasts expressed angiotensin II, AT1R, and AT2R. Tear secretion increased in mice that received ARB. CONCLUSIONS. The results are consistent with the hypothesis that a tissue-specific RAS is present in the lacrimal gland, and suggest that fibroblasts are one of the cell types playing a role in the tissue RAS. Tissue RAS might be involved in tissue function of regulating tear secretion in the lacrimal gland.

Original languageEnglish
Pages (from-to)5416-5425
Number of pages10
JournalInvestigative Ophthalmology and Visual Science
Volume53
Issue number9
DOIs
Publication statusPublished - 2012 Aug

Fingerprint

Lacrimal Apparatus
Renin-Angiotensin System
Angiotensin Type 2 Receptor
Angiotensin Type 1 Receptor
Tears
Renin
Fibroblasts
Peptidyl-Dipeptidase A
Angiotensin II
Blood Vessels
Angiotensin II Type 2 Receptor Blockers
Angiotensin II Type 1 Receptor Blockers
Angiotensinogen
Reverse Transcription
Immunohistochemistry
Polymerase Chain Reaction
Messenger RNA

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience
  • Medicine(all)

Cite this

Presence and physiologic function of the renin-angiotensin system in mouse lacrimal gland. / Yaguchi, Saori; Ogawa, Yoko; Shimmura, Shigeto; Hatou, Shin; Nakamura, Shigeru; Inaba, Takaaki; Imada, Toshihiro; Ozawa, Yoko; Kawakami, Yutaka; Ishida, Susumu; Tsubota, Kazuo.

In: Investigative Ophthalmology and Visual Science, Vol. 53, No. 9, 08.2012, p. 5416-5425.

Research output: Contribution to journalArticle

Yaguchi, Saori ; Ogawa, Yoko ; Shimmura, Shigeto ; Hatou, Shin ; Nakamura, Shigeru ; Inaba, Takaaki ; Imada, Toshihiro ; Ozawa, Yoko ; Kawakami, Yutaka ; Ishida, Susumu ; Tsubota, Kazuo. / Presence and physiologic function of the renin-angiotensin system in mouse lacrimal gland. In: Investigative Ophthalmology and Visual Science. 2012 ; Vol. 53, No. 9. pp. 5416-5425.
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T1 - Presence and physiologic function of the renin-angiotensin system in mouse lacrimal gland

AU - Yaguchi, Saori

AU - Ogawa, Yoko

AU - Shimmura, Shigeto

AU - Hatou, Shin

AU - Nakamura, Shigeru

AU - Inaba, Takaaki

AU - Imada, Toshihiro

AU - Ozawa, Yoko

AU - Kawakami, Yutaka

AU - Ishida, Susumu

AU - Tsubota, Kazuo

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N2 - PURPOSE. To investigate the expression, localization, and physiologic function of renin-angiotensin system (RAS) components in the mouse lacrimal gland. METHODS. Lacrimal glands and cultured lacrimal gland fibroblasts from wild-type (WT) BALB/c (H-2d) mice were used. Reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry were used to determine the expression and localization of the RAS components, prorenin/renin, angiotensin-converting enzyme (ACE), angiotensin II, angiotensin II type 1 receptor (AT1R), and angiotensin II type 2 receptor (AT2R) in the normal mouse lacrimal gland. To examine the change in tear secretion, mice received ARB (AT1R blocker) or AT2R antagonist. Tear secretion was assessed by cotton thread test before and after drug administration. RESULTS. The mRNAs coding for angiotensinogen, prorenin, ACE, and both AT1R and AT2R were found in normal lacrimal gland tissue and cultured lacrimal gland fibroblasts. Prorenin/renin and ACE were identified in myoepithelial cells around ducts and acini and in blood vessels. Angiotensin II, AT1R, and AT2R were observed in the ducts and interstitial fibroblasts. AT1R and AT2R were also localized in blood vessels. All the cultured lacrimal gland fibroblasts expressed angiotensin II, AT1R, and AT2R. Tear secretion increased in mice that received ARB. CONCLUSIONS. The results are consistent with the hypothesis that a tissue-specific RAS is present in the lacrimal gland, and suggest that fibroblasts are one of the cell types playing a role in the tissue RAS. Tissue RAS might be involved in tissue function of regulating tear secretion in the lacrimal gland.

AB - PURPOSE. To investigate the expression, localization, and physiologic function of renin-angiotensin system (RAS) components in the mouse lacrimal gland. METHODS. Lacrimal glands and cultured lacrimal gland fibroblasts from wild-type (WT) BALB/c (H-2d) mice were used. Reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry were used to determine the expression and localization of the RAS components, prorenin/renin, angiotensin-converting enzyme (ACE), angiotensin II, angiotensin II type 1 receptor (AT1R), and angiotensin II type 2 receptor (AT2R) in the normal mouse lacrimal gland. To examine the change in tear secretion, mice received ARB (AT1R blocker) or AT2R antagonist. Tear secretion was assessed by cotton thread test before and after drug administration. RESULTS. The mRNAs coding for angiotensinogen, prorenin, ACE, and both AT1R and AT2R were found in normal lacrimal gland tissue and cultured lacrimal gland fibroblasts. Prorenin/renin and ACE were identified in myoepithelial cells around ducts and acini and in blood vessels. Angiotensin II, AT1R, and AT2R were observed in the ducts and interstitial fibroblasts. AT1R and AT2R were also localized in blood vessels. All the cultured lacrimal gland fibroblasts expressed angiotensin II, AT1R, and AT2R. Tear secretion increased in mice that received ARB. CONCLUSIONS. The results are consistent with the hypothesis that a tissue-specific RAS is present in the lacrimal gland, and suggest that fibroblasts are one of the cell types playing a role in the tissue RAS. Tissue RAS might be involved in tissue function of regulating tear secretion in the lacrimal gland.

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