Abstract
To clarify the presence and origin of acetylcholine (ACh) in human blood, the ACh contents of whole blood, mononuclear (MNL) and polymorphonuclear leukocytes (PMN) were determined by a sensitive, specific radioimmunoassay. The mean (± S.E.M.) ACh contents of whole blood and MNL measured in 30 normal subjects were 1263.5 ± 149.0 and 731.0 ± 85.8 pg/ml blood, respectively. A significant correlation was observed between the ACh content of whole blood and that of MNL (r = 0.91; P < 0.001). No detectable ACh was present in PMN. These results demonstrate that ACh is present in human blood, and indicate that the major portion of plasma ACh originates from circulating MNL.
| Original language | English |
|---|---|
| Pages (from-to) | 251-258 |
| Number of pages | 8 |
| Journal | Biogenic Amines |
| Volume | 9 |
| Issue number | 4 |
| Publication status | Published - 1993 |
Fingerprint
Keywords
- Acetylcholine
- Human blood
- Mononuclear leukocytes
- Radioimmunoassay
ASJC Scopus subject areas
- Neuroscience(all)
- Pharmacology
Cite this
Presence of acetylcholine in blood and its localization in circulating mononuclear leukocytes of humans. / Kawashima, K.; Kajiyama, K.; Fujimoto, Kazuko; Oohata, H.; Suzuki, Takeshi.
In: Biogenic Amines, Vol. 9, No. 4, 1993, p. 251-258.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Presence of acetylcholine in blood and its localization in circulating mononuclear leukocytes of humans
AU - Kawashima, K.
AU - Kajiyama, K.
AU - Fujimoto, Kazuko
AU - Oohata, H.
AU - Suzuki, Takeshi
PY - 1993
Y1 - 1993
N2 - To clarify the presence and origin of acetylcholine (ACh) in human blood, the ACh contents of whole blood, mononuclear (MNL) and polymorphonuclear leukocytes (PMN) were determined by a sensitive, specific radioimmunoassay. The mean (± S.E.M.) ACh contents of whole blood and MNL measured in 30 normal subjects were 1263.5 ± 149.0 and 731.0 ± 85.8 pg/ml blood, respectively. A significant correlation was observed between the ACh content of whole blood and that of MNL (r = 0.91; P < 0.001). No detectable ACh was present in PMN. These results demonstrate that ACh is present in human blood, and indicate that the major portion of plasma ACh originates from circulating MNL.
AB - To clarify the presence and origin of acetylcholine (ACh) in human blood, the ACh contents of whole blood, mononuclear (MNL) and polymorphonuclear leukocytes (PMN) were determined by a sensitive, specific radioimmunoassay. The mean (± S.E.M.) ACh contents of whole blood and MNL measured in 30 normal subjects were 1263.5 ± 149.0 and 731.0 ± 85.8 pg/ml blood, respectively. A significant correlation was observed between the ACh content of whole blood and that of MNL (r = 0.91; P < 0.001). No detectable ACh was present in PMN. These results demonstrate that ACh is present in human blood, and indicate that the major portion of plasma ACh originates from circulating MNL.
KW - Acetylcholine
KW - Human blood
KW - Mononuclear leukocytes
KW - Radioimmunoassay
UR - http://www.scopus.com/inward/record.url?scp=0027272191&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027272191&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0027272191
VL - 9
SP - 251
EP - 258
JO - Biogenic Amines
JF - Biogenic Amines
SN - 0168-8561
IS - 4
ER -