Presence of acetylcholine in blood and its localization in circulating mononuclear leukocytes of humans

K. Kawashima, K. Kajiyama, K. Fujimoto, H. Oohata, T. Suzuki

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To clarify the presence and origin of acetylcholine (ACh) in human blood, the ACh contents of whole blood, mononuclear (MNL) and polymorphonuclear leukocytes (PMN) were determined by a sensitive, specific radioimmunoassay. The mean (± S.E.M.) ACh contents of whole blood and MNL measured in 30 normal subjects were 1263.5 ± 149.0 and 731.0 ± 85.8 pg/ml blood, respectively. A significant correlation was observed between the ACh content of whole blood and that of MNL (r = 0.91; P < 0.001). No detectable ACh was present in PMN. These results demonstrate that ACh is present in human blood, and indicate that the major portion of plasma ACh originates from circulating MNL.

Original languageEnglish
Pages (from-to)251-258
Number of pages8
JournalBiogenic Amines
Issue number4
Publication statusPublished - 1993 Jan 1



  • Acetylcholine
  • Human blood
  • Mononuclear leukocytes
  • Radioimmunoassay

ASJC Scopus subject areas

  • Neuroscience(all)
  • Pharmacology

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