Presence of acetylcholine in blood and its localization in circulating mononuclear leukocytes of humans

K. Kawashima; K. Kajiyama; K. Fujimoto; H. Oohata; T. Suzuki

Presence of acetylcholine in blood and its localization in circulating mononuclear leukocytes of humans

K. Kawashima, K. Kajiyama, K. Fujimoto, H. Oohata, T. Suzuki

School of Pharmacy

Research output: Contribution to journal › Article › peer-review

43 Citations (Scopus)

Abstract

To clarify the presence and origin of acetylcholine (ACh) in human blood, the ACh contents of whole blood, mononuclear (MNL) and polymorphonuclear leukocytes (PMN) were determined by a sensitive, specific radioimmunoassay. The mean (± S.E.M.) ACh contents of whole blood and MNL measured in 30 normal subjects were 1263.5 ± 149.0 and 731.0 ± 85.8 pg/ml blood, respectively. A significant correlation was observed between the ACh content of whole blood and that of MNL (r = 0.91; P < 0.001). No detectable ACh was present in PMN. These results demonstrate that ACh is present in human blood, and indicate that the major portion of plasma ACh originates from circulating MNL.

Original language	English
Pages (from-to)	251-258
Number of pages	8
Journal	Biogenic Amines
Volume	9
Issue number	4
Publication status	Published - 1993 Jan 1

Keywords

Acetylcholine
Human blood
Mononuclear leukocytes
Radioimmunoassay

ASJC Scopus subject areas

Neuroscience(all)
Pharmacology

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abstract = "To clarify the presence and origin of acetylcholine (ACh) in human blood, the ACh contents of whole blood, mononuclear (MNL) and polymorphonuclear leukocytes (PMN) were determined by a sensitive, specific radioimmunoassay. The mean (± S.E.M.) ACh contents of whole blood and MNL measured in 30 normal subjects were 1263.5 ± 149.0 and 731.0 ± 85.8 pg/ml blood, respectively. A significant correlation was observed between the ACh content of whole blood and that of MNL (r = 0.91; P < 0.001). No detectable ACh was present in PMN. These results demonstrate that ACh is present in human blood, and indicate that the major portion of plasma ACh originates from circulating MNL.",

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T1 - Presence of acetylcholine in blood and its localization in circulating mononuclear leukocytes of humans

AU - Kawashima, K.

AU - Kajiyama, K.

AU - Fujimoto, K.

AU - Oohata, H.

AU - Suzuki, T.

PY - 1993/1/1

Y1 - 1993/1/1

N2 - To clarify the presence and origin of acetylcholine (ACh) in human blood, the ACh contents of whole blood, mononuclear (MNL) and polymorphonuclear leukocytes (PMN) were determined by a sensitive, specific radioimmunoassay. The mean (± S.E.M.) ACh contents of whole blood and MNL measured in 30 normal subjects were 1263.5 ± 149.0 and 731.0 ± 85.8 pg/ml blood, respectively. A significant correlation was observed between the ACh content of whole blood and that of MNL (r = 0.91; P < 0.001). No detectable ACh was present in PMN. These results demonstrate that ACh is present in human blood, and indicate that the major portion of plasma ACh originates from circulating MNL.

AB - To clarify the presence and origin of acetylcholine (ACh) in human blood, the ACh contents of whole blood, mononuclear (MNL) and polymorphonuclear leukocytes (PMN) were determined by a sensitive, specific radioimmunoassay. The mean (± S.E.M.) ACh contents of whole blood and MNL measured in 30 normal subjects were 1263.5 ± 149.0 and 731.0 ± 85.8 pg/ml blood, respectively. A significant correlation was observed between the ACh content of whole blood and that of MNL (r = 0.91; P < 0.001). No detectable ACh was present in PMN. These results demonstrate that ACh is present in human blood, and indicate that the major portion of plasma ACh originates from circulating MNL.

KW - Acetylcholine

KW - Human blood

KW - Mononuclear leukocytes

KW - Radioimmunoassay

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