Preservation of hematopoietic properties in transplanted bone marrow cells in the brain

Kenji Ono, Ken Yoshihara, Hiromi Suzuki, Kenji Tanaka, Takemasa Takii, Kikuo Onozaki, Makoto Sawada

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Recent studies have described the possible transdifferentiation of bone marrow cells (BMC) into neurons and glia when they migrate to the brain. However, we have reported that some immature BMC migrating into the brain parenchyma after bone marrow transplantation express early hematopoietic markers but not neural or glial markers. The present study further characterizes transplanted BMC that migrate to the brain. Double immunolabeling confirmed that BMC migrating to the brain expressed hematopoietic but not neural markers, such as nestin, microtubule-associated protein-2 and glial fibrillary acidic protein, even 4 and 18 weeks after bone marrow transplantation. BMC that expressed green fluorescent protein also expressed hematopoietic but not neural markers when cultured with mixed brain cells according to double immunolabeling and single-cell dissection using a laser. Analysis of the DNA content indicated that most of the migrated BMC were arrested at the G0/G1 phase, and aneuploidy or tetraploidy was undetectable. Thus, BMC that migrate to the brain probably have preserved hematopoietic properties under physiological conditions.

Original languageEnglish
Pages (from-to)503-507
Number of pages5
JournalJournal of Neuroscience Research
Volume72
Issue number4
DOIs
Publication statusPublished - 2003 May 15
Externally publishedYes

Fingerprint

Bone Marrow Cells
Brain
Bone Marrow Transplantation
Neuroglia
Nestin
Cell Cycle Resting Phase
Microtubule-Associated Proteins
Tetraploidy
Glial Fibrillary Acidic Protein
G1 Phase
Aneuploidy
Green Fluorescent Proteins
Dissection
Lasers
Neurons
DNA

Keywords

  • Bone marrow transplantation
  • ER-MP12
  • Green fluorescent protein
  • Microglia
  • Transdifferentiation

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Preservation of hematopoietic properties in transplanted bone marrow cells in the brain. / Ono, Kenji; Yoshihara, Ken; Suzuki, Hiromi; Tanaka, Kenji; Takii, Takemasa; Onozaki, Kikuo; Sawada, Makoto.

In: Journal of Neuroscience Research, Vol. 72, No. 4, 15.05.2003, p. 503-507.

Research output: Contribution to journalArticle

Ono, Kenji ; Yoshihara, Ken ; Suzuki, Hiromi ; Tanaka, Kenji ; Takii, Takemasa ; Onozaki, Kikuo ; Sawada, Makoto. / Preservation of hematopoietic properties in transplanted bone marrow cells in the brain. In: Journal of Neuroscience Research. 2003 ; Vol. 72, No. 4. pp. 503-507.
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