Presynaptic ionotropic glutamate receptors modulate in vivo release and metabolism of striatal dopamine, noradrenaline, and 5-hydroxytryptamine: involvement of both NMDA and AMPA/kainate subtypes

Kouichi Ohta, Nobuo Araki, Mamoru Shibata, Satoru Komatsumoto, Kunio Shimazu, Yasuo Fukuuchi

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

In order to explore further the presynaptic modulation of monoamine release by glutamatergic nerve fibers, we investigated the effects of selective agonists for ionotropic glutamate (GLU) receptors on striatal release of dopamine (DA), noradrenaline (NA) and 5-hydroxytryptamine (5-HT). In the striatum of anesthetized Sprague-Dawley rats, in vivo microdialysis was performed to measure the release of monoamines and metabolites, and also to administer GLU agonists locally in the tissue. l-GLU and its selective agonists (N-methyl-d-aspartate (NMDA), α-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid (AMPA) and kainate (KA)) evoked simultaneous release of striatal DA, NA and 5-HT in a dose-dependent manner. Pretreatment with MK-801 (5 mg/kg i.p.), a noncompetitive NMDA receptor antagonist, selectively suppressed NMDA-evoked monoamine release. The rank order of GLU agonist efficacy in releasing monoamines was different among DA, NA, and 5-HTergic terminals: AMPA = KA > NMDA for DA release, AMPA > NMDA = KA for NA release, and NMDA = AMPA = KA for 5-HT release. In conclusion, presynaptic ionotropic GLU receptors exist extensively on monoaminergic terminals including not only catecholaminergic (DA and NA) but also indoleaminergic (5-HT) terminals in the rat striatum. Their subtypes include both NMDA subtype and AMPA/KA subtype, and show a differential distribution among these three monoaminergic terminals and a differential contribution to facilitating monoamine release.

Original languageEnglish
Pages (from-to)83-89
Number of pages7
JournalNeuroscience Research
Volume21
Issue number1
DOIs
Publication statusPublished - 1994

Fingerprint

Ionotropic Glutamate Receptors
Corpus Striatum
alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
Kainic Acid
Aspartic Acid
Dopamine
Serotonin
Norepinephrine
Excitatory Amino Acid Agonists
Isoxazoles
Dizocilpine Maleate
Microdialysis
Nerve Fibers
Sprague Dawley Rats
Glutamic Acid

Keywords

  • Catecholamine
  • Excitatory amino acid
  • Glutamate receptor
  • Indoleamine
  • Kainate
  • Microdialysis
  • N-Methyl-d-aspartate
  • α-Amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Presynaptic ionotropic glutamate receptors modulate in vivo release and metabolism of striatal dopamine, noradrenaline, and 5-hydroxytryptamine : involvement of both NMDA and AMPA/kainate subtypes. / Ohta, Kouichi; Araki, Nobuo; Shibata, Mamoru; Komatsumoto, Satoru; Shimazu, Kunio; Fukuuchi, Yasuo.

In: Neuroscience Research, Vol. 21, No. 1, 1994, p. 83-89.

Research output: Contribution to journalArticle

@article{05d4f72b6139468f85ad667c7371e644,
title = "Presynaptic ionotropic glutamate receptors modulate in vivo release and metabolism of striatal dopamine, noradrenaline, and 5-hydroxytryptamine: involvement of both NMDA and AMPA/kainate subtypes",
abstract = "In order to explore further the presynaptic modulation of monoamine release by glutamatergic nerve fibers, we investigated the effects of selective agonists for ionotropic glutamate (GLU) receptors on striatal release of dopamine (DA), noradrenaline (NA) and 5-hydroxytryptamine (5-HT). In the striatum of anesthetized Sprague-Dawley rats, in vivo microdialysis was performed to measure the release of monoamines and metabolites, and also to administer GLU agonists locally in the tissue. l-GLU and its selective agonists (N-methyl-d-aspartate (NMDA), α-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid (AMPA) and kainate (KA)) evoked simultaneous release of striatal DA, NA and 5-HT in a dose-dependent manner. Pretreatment with MK-801 (5 mg/kg i.p.), a noncompetitive NMDA receptor antagonist, selectively suppressed NMDA-evoked monoamine release. The rank order of GLU agonist efficacy in releasing monoamines was different among DA, NA, and 5-HTergic terminals: AMPA = KA > NMDA for DA release, AMPA > NMDA = KA for NA release, and NMDA = AMPA = KA for 5-HT release. In conclusion, presynaptic ionotropic GLU receptors exist extensively on monoaminergic terminals including not only catecholaminergic (DA and NA) but also indoleaminergic (5-HT) terminals in the rat striatum. Their subtypes include both NMDA subtype and AMPA/KA subtype, and show a differential distribution among these three monoaminergic terminals and a differential contribution to facilitating monoamine release.",
keywords = "Catecholamine, Excitatory amino acid, Glutamate receptor, Indoleamine, Kainate, Microdialysis, N-Methyl-d-aspartate, α-Amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid",
author = "Kouichi Ohta and Nobuo Araki and Mamoru Shibata and Satoru Komatsumoto and Kunio Shimazu and Yasuo Fukuuchi",
year = "1994",
doi = "10.1016/0168-0102(94)90071-X",
language = "English",
volume = "21",
pages = "83--89",
journal = "Neuroscience Research",
issn = "0168-0102",
publisher = "Elsevier Ireland Ltd",
number = "1",

}

TY - JOUR

T1 - Presynaptic ionotropic glutamate receptors modulate in vivo release and metabolism of striatal dopamine, noradrenaline, and 5-hydroxytryptamine

T2 - involvement of both NMDA and AMPA/kainate subtypes

AU - Ohta, Kouichi

AU - Araki, Nobuo

AU - Shibata, Mamoru

AU - Komatsumoto, Satoru

AU - Shimazu, Kunio

AU - Fukuuchi, Yasuo

PY - 1994

Y1 - 1994

N2 - In order to explore further the presynaptic modulation of monoamine release by glutamatergic nerve fibers, we investigated the effects of selective agonists for ionotropic glutamate (GLU) receptors on striatal release of dopamine (DA), noradrenaline (NA) and 5-hydroxytryptamine (5-HT). In the striatum of anesthetized Sprague-Dawley rats, in vivo microdialysis was performed to measure the release of monoamines and metabolites, and also to administer GLU agonists locally in the tissue. l-GLU and its selective agonists (N-methyl-d-aspartate (NMDA), α-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid (AMPA) and kainate (KA)) evoked simultaneous release of striatal DA, NA and 5-HT in a dose-dependent manner. Pretreatment with MK-801 (5 mg/kg i.p.), a noncompetitive NMDA receptor antagonist, selectively suppressed NMDA-evoked monoamine release. The rank order of GLU agonist efficacy in releasing monoamines was different among DA, NA, and 5-HTergic terminals: AMPA = KA > NMDA for DA release, AMPA > NMDA = KA for NA release, and NMDA = AMPA = KA for 5-HT release. In conclusion, presynaptic ionotropic GLU receptors exist extensively on monoaminergic terminals including not only catecholaminergic (DA and NA) but also indoleaminergic (5-HT) terminals in the rat striatum. Their subtypes include both NMDA subtype and AMPA/KA subtype, and show a differential distribution among these three monoaminergic terminals and a differential contribution to facilitating monoamine release.

AB - In order to explore further the presynaptic modulation of monoamine release by glutamatergic nerve fibers, we investigated the effects of selective agonists for ionotropic glutamate (GLU) receptors on striatal release of dopamine (DA), noradrenaline (NA) and 5-hydroxytryptamine (5-HT). In the striatum of anesthetized Sprague-Dawley rats, in vivo microdialysis was performed to measure the release of monoamines and metabolites, and also to administer GLU agonists locally in the tissue. l-GLU and its selective agonists (N-methyl-d-aspartate (NMDA), α-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid (AMPA) and kainate (KA)) evoked simultaneous release of striatal DA, NA and 5-HT in a dose-dependent manner. Pretreatment with MK-801 (5 mg/kg i.p.), a noncompetitive NMDA receptor antagonist, selectively suppressed NMDA-evoked monoamine release. The rank order of GLU agonist efficacy in releasing monoamines was different among DA, NA, and 5-HTergic terminals: AMPA = KA > NMDA for DA release, AMPA > NMDA = KA for NA release, and NMDA = AMPA = KA for 5-HT release. In conclusion, presynaptic ionotropic GLU receptors exist extensively on monoaminergic terminals including not only catecholaminergic (DA and NA) but also indoleaminergic (5-HT) terminals in the rat striatum. Their subtypes include both NMDA subtype and AMPA/KA subtype, and show a differential distribution among these three monoaminergic terminals and a differential contribution to facilitating monoamine release.

KW - Catecholamine

KW - Excitatory amino acid

KW - Glutamate receptor

KW - Indoleamine

KW - Kainate

KW - Microdialysis

KW - N-Methyl-d-aspartate

KW - α-Amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid

UR - http://www.scopus.com/inward/record.url?scp=0028597548&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028597548&partnerID=8YFLogxK

U2 - 10.1016/0168-0102(94)90071-X

DO - 10.1016/0168-0102(94)90071-X

M3 - Article

C2 - 7535906

AN - SCOPUS:0028597548

VL - 21

SP - 83

EP - 89

JO - Neuroscience Research

JF - Neuroscience Research

SN - 0168-0102

IS - 1

ER -